FastQCFastQC Report
Tue 19 Sept 2023
SRR16796961_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16796961_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73935242
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT13909211.8812692869795435Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAG13072941.7681608454057676Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT9688471.3103994438809032Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT7800111.0549921511043407Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC7564051.023064210704822Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG6420700.8684221254053649Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG5469630.7397865824257396TruSeq Adapter, Index 1 (100% over 49bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA5043970.6822145790771876Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCAGGGGTTAGAGCACTGGAGATCGGAAGAGCACACGTCTGAACTCCAG5038690.6815004406153158Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC4944950.6688217778471599Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4848280.6557468223340637Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4513870.6105167005472167Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT4125300.5579612493863211Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCC4070430.55053989002971Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT3659490.4949588181506189No Hit
GAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCA3558940.4813590790708442Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG3504700.47402292941707014Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTC3100470.4193494084999411Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA2980460.4031176363769797Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGT2490620.33686506361878144TruSeq Adapter, Index 1 (100% over 50bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA2273690.30752452260858226Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1960050.2651036159454242Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA1951560.26395531375957354Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGACCCGGTGAGGCGGGAAGATCGGAAGAGCACACGTCTGAACTCCAG1873720.25342718158682703Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1618750.21894159756723322Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC1594950.21572256434894743Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTGGTCCGATGGTAGTGAGTTATCTAGATCGGAAGAGCACACGTCTGAA1557130.21060727710879742Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAA1508550.20403666224559056Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGCTCTCGCTTCTGGCGCCAGATCGGAAGAGCACACGTCTGAACTCCA1502030.2031548094479761Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG1452590.1964678765777219Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTGA1413160.19113483120809965Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC1404790.19000275944183695Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGGTTGGCCCCGGATAGCCGGTTCCCAGATCGGAAGAGCACACGTCTG1396930.18893966695882322Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTCTGAACTCCAG1376500.18617643802396697Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC1358740.1837743359249436No Hit
GGGCTCTCGCTTCTGGCGCCAGATCGGAAGAGCACACGTCTGAACTCCAG1343600.1817265979869248Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT1338650.18105709317892002Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC1255450.16980400226457634No Hit
AATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1212660.16401650514649022Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAAC1211810.1639015396744086Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGATACCCTTTATGAAATTAAAGGACAGATCGGAAGAGCACACGTCTGA1200700.16239887332755332Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTCCA1175670.15901347830849055Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGGTTGGCCCCGGATAGCCGGTTCCCAGATCGGAAGAGCACACGTCTGA1167850.1579557959653395Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACTCCAG966050.13066164035819347Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGTCGGTTTCTATCTATTTACGATTTCTCAGATCGGAAGAGCACACGTC915330.12380158301233395No Hit
ATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCA900060.1217362621197615Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGGAAGGTGGCTCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA875140.11836574498532107Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGTC853070.11538070031609554No Hit
CGGGGGAAGGTGGCTCGGGGGGGGAGATCGGAAGAGCACACGTCTGAACT846770.11452860328772577Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGGGGAAGGTGGCTCGGGGGGGGAGATCGGAAGAGCACACGTCTGAAC840520.11368326893418433Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT831150.11241594367135499Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG801590.1084178503128454Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTG782310.10581016289904076Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGTTAGTGGGGAGATCGGAAGAGCACACGTCTGA750740.10154021001243224Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGTCGGTTTCTATCTATTTACGATTTCTCCAGATCGGAAGAGCACACGT745770.10086800013449607No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)