FastQCFastQC Report
Tue 19 Sept 2023
SRR16796962_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16796962_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49302676
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC10304702.090089389874091No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT9530201.9329985252727457Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG7139911.4481789994522813Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA6970361.4137893853875194Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG4491260.910956638540269Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA4067260.8249572497849812Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT3782340.7671672831714044Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT3071460.6229803834582934Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG2721620.5520227745852984TruSeq Adapter, Index 9 (100% over 49bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA2688280.5452604641581726No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG2608000.528977372343846Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA2520690.5112683944376569Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA2195860.44538353252874147Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC1874430.3801882883598448Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGA1765650.35812457725418395Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA1607600.32606749378066213Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC1419020.28781804865926547Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA1308100.26532028403488683Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1253350.2542154101331133No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC1243340.25218509437499903Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1154490.2341637601983308No Hit
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG1141780.23158580682314284Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC1139930.23121057364107375No Hit
TCGACTGCATAATTTGTGTTAGTGGGGAGATCGGAAGAGCACACGTCTGA1064770.2159659650117166Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG992520.2013115880363167No Hit
AGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAG982890.19935834720208695Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCTGGTCCGATGGTAGTGAGTTATCTAGATCGGAAGAGCACACGTCTGAA973440.19744161554232875Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT933130.18926558874816451No Hit
GGGGCCCGAGGTGGGATCCCGAGGCCTAGATCGGAAGAGCACACGTCTGA932830.1892047401240452Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTC929980.18862667819491177Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA924970.18761050617211933Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGTC836230.1696114831576282No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC829950.16833771862606403No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGT781100.15842953433197013TruSeq Adapter, Index 9 (100% over 50bp)
AGGTCGGTTTCTATCTATTTACGATTTCTCAGATCGGAAGAGCACACGTC773000.15678662148074884No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACAC707220.14344454649885535No Hit
AGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCC693770.14071649985083973Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGTGCCTAGTGGGCCACTTTTGGTAAGCAGATCGGAAGAGCACACGTCT682320.13839411069695284Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAACCGAAGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTC678420.13760307858340184No Hit
TAAACTCTAAGGAGAGGGTATCAAGCAGATCGGAAGAGCACACGTCTGAA678020.1375219470845761Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGCCCGAGGTGGGATCCCGAGGCCTAGATCGGAAGAGCACACGTCTGAA673980.1367025189464361Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA651590.1321611832996651Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACGTCTGAA650490.13193807167789431Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAAACCGAAGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCT642560.13032963971367395Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT641910.13019780102808212No Hit
GCTGGTCCGATGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCT640490.12990978420725074Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGATAACTGTGGTAATTCTAGAGCTAATACAAGATCGGAAGAGCACACG634910.1287779997986316No Hit
AACTTTCGATGGTAGTCGATGTGCCTACCAGATCGGAAGAGCACACGTCT629770.1277354600387208Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAAGGGCAGGGCGCCCTGGAATGGGTTAGATCGGAAGAGCACACGTCTG589060.1194783017457308Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT578910.11741958996302757Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTCCATAGCTCAGGGGTTAGAGCACTGGAGATCGGAAGAGCACACGTC536440.10880545307520428No Hit
ACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAA515170.10449128562514537Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC505470.1025238467786211No Hit
TGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG499390.10129064799646981Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)