FastQCFastQC Report
Tue 19 Sept 2023
SRR16796963_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16796963_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37208000
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT12269593.297567727370458Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC5362991.4413540098903461Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT5356621.4396420124704365Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC5112641.3740700924532359Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG4655481.251204042141475Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATG4544321.2213287465061278TruSeq Adapter, Index 21 (97% over 49bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC4360321.1718770156955494No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC4163861.1190765426789937Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA3233630.8690684798967964Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG3072010.8256315846054612Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAG2988290.8031310470866481Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT2559310.6878386368522899Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT2535260.6813749731240594No Hit
CTCAGGGGTTAGAGCACTGGAGATCGGAAGAGCACACGTCTGAACTCCAG2445360.6572135024725865Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC2273710.6110809503332617Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT2217260.5959094818318641Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT2144430.5763357342506987No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT1981910.5326569554934423Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTCCATAGCTCAGGGGTTAGAGCACTGGAGATCGGAAGAGCACACGTC1876360.5042894001290046No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1654470.4446543754031391No Hit
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA1621690.4358444420554719Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT1518570.4081299720490217Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG1501080.40342937002795104Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTCTGAACTCCA1478500.39736078262739194Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1463310.3932783272414534Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC1431780.38480434315201034No Hit
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC1379450.3707401634057192Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1344080.36123414319501185Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC1303280.3502687594065792No Hit
CTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1142680.30710599870995486Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC1015870.27302461836164266Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA981140.263690604171146Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG953570.2562809073317566Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA916160.24622661793162762Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA907450.24388572350032253Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCC901360.242248978714255Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCA883890.23755375188131583Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACTCCAG828890.22277198451945818Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT778360.20919157170500965No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGT750190.20162061922167276No Hit
AGGTCGGTTTCTATCTATTTACGATTTCTCCAGATCGGAAGAGCACACGT737770.1982826273919587No Hit
GTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTGAACTCCA733720.19719415179531283Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTCCCTCCCGTGGTTTCTCTCTCTCCCGGAGATCGGAAGAGCACACGTC710840.19104493657278004No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGCCGT664510.1785933132659643TruSeq Adapter, Index 21 (98% over 50bp)
CAGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTC654420.17588153085357988Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC649710.17461567404859168Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCCCTCCCGTGGTTTCTCTCTCTCCCGGAGATCGGAAGAGCACACGTCT618130.16612825198881961Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGT614140.16505590195656847No Hit
GTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGA600460.1613792732745646Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGTCTG586900.15773489572135024Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCAGCGGAAGCGTGCTGGGAGATCGGAAGAGCACACGTCTGAACTCCA585530.1573666953343367Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT564450.15170124704364651No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA546670.14692270479466782No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC530100.14246936142765No Hit
TACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG510230.13712911201892067Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGGGGAAGGTGGCTCGGGGGGGGAGATCGGAAGAGCACACGTCTGAAC476540.12807460761126638Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC447540.12028058482046872No Hit
ACGATACCCTTTATGAAATTAAAGGACAGATCGGAAGAGCACACGTCTGA439780.11819501182541389Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC423500.11381960868630402No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC422480.11354547409159321No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGT415680.11171791012685443No Hit
TCGACTGCATAATTTGTGTTAGTGGGGAGATCGGAAGAGCACACGTCTGA413950.11125295635347238Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGGAAGGTGGCTCGGGGGGGGAGATCGGAAGAGCACACGTCTGAACT413220.11105676198666953Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATCGGAAGAGCACACGTCTG407960.10964308750806277Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG406330.10920500967533864Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAA403190.10836110513867986Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG392660.10553106858740056No Hit
GAAACCGAAGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCT389980.10481079337776823Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGTCGGTTTCTATCTATTTACGATTTCTCAGATCGGAAGAGCACACGTC384710.10339443130509568No Hit
ATAGCTCAGGGGTTAGAGCACTGGAGATCGGAAGAGCACACGTCTGAACT373720.10044076542678994Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)