FastQCFastQC Report
Tue 19 Sept 2023
SRR16796964_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16796964_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52337425
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT30479525.823656780974608Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC24119824.6085224865380745Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC17753083.3920430743392513Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT10595712.024499676856475Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC9627291.8394657360387905Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA9488031.8128576253034994Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT7380081.4100961214656624Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG6990081.3355796545206418Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC6823021.3036598571672184No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC6094151.164396223161533No Hit
ACGGGGGAAGGTGGCTCGGGGGGGGAGATCGGAAGAGCACACGTCTGAAC5809811.1100679867226941Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG5570171.0642804838029385Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT4667490.8918073443620889Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTCCATAGCTCAGGGGTTAGAGCACTGGAGATCGGAAGAGCACACGTC4477270.855462415279315No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT4343920.8299835156200367Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT4261710.8142758265237542No Hit
CTCGGTTGGCCCCGGATAGCCGGTTCCCCGAGATCGGAAGAGCACACGTC3831210.7320211110882892No Hit
GTCCCTCCCGTGGTTTCTCTCTCTCCCGGAGATCGGAAGAGCACACGTCT3644060.6962627603478773Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT3240960.6192433043849598No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT3050220.5827990200129257No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC2488850.4755392532208071No Hit
CCTCGGTTGGCCCCGGATAGCCGGTTCCCCGAGATCGGAAGAGCACACGT2440380.46627819385458874No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGT2434040.46506682359707224No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACG2310720.44150433461332883No Hit
GTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC2245390.429021871060718Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC2236990.4274169010034407No Hit
GTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTCTGAACTCCA2180080.4165432288653865Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTCCCTCCCGTGGTTTCTCTCTCTCCCGGAGATCGGAAGAGCACACGTC2097700.4008030582322306No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA2072830.3960512004555058Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGGAAGGTGGCTCGGGGGGGGAGATCGGAAGAGCACACGTCTGAACT2000130.3821605667454981Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1976620.377668561263761No Hit
CTCAGGGGTTAGAGCACTGGAGATCGGAAGAGCACACGTCTGAACTCCAG1896290.3623200797517264Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTCTAGCGGTTAGGATTCCTGAGATCGGAAGAGCACACGTCTGAACTCCA1824360.34857656829696915Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGTCTG1760670.3364074560412554Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG1598060.30533791068246097Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGTGCATTTATCAGATCAAAACCAACCCGGAGATCGGAAGAGCACACG1431250.2734658802950279No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGT1427490.2727474651265323No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGT1323140.25280953352214025No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT1320120.2522325085729762Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG1271960.243030680244586TruSeq Adapter, Index 4 (100% over 49bp)
AGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAG1266520.24199127106463492Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTG1237060.23636241179232645Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGCATTTATCAGATCAAAACCAACCCGGAGATCGGAAGAGCACACGTCT1207350.23068578555402755Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCATTTATCAGATCAAAACCAACCCGGTAGATCGGAAGAGCACACGTC1203590.22996737038553194No Hit
TGCGTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCT1159120.22147058247516Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1098380.2098651204181329No Hit
CCTCGGTTGGCCCCGGATAGCCGGTTCCCAGATCGGAAGAGCACACGTCT1058130.20217463889367887Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAA1056120.20179059248711606Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG1041020.19890546774129605Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTGAACTCCA1038400.19840486993771664Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC1001980.19144617833223548No Hit
GTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGA992200.1895775346226911Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATAGCTCAGGGGTTAGAGCACTGGAGATCGGAAGAGCACACGTCTGAACT967480.18485433702556056Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG955670.1825978255521742No Hit
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGTC902270.1723948016166252No Hit
AGGTCGGTTTCTATCTATTTACGATTTCTCCAGATCGGAAGAGCACACGT902140.1723699627943102No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTAGATCGGAAGAGCACAC884930.1690816848555312No Hit
GTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT877670.16769453216317004Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC836280.1597862332737998No Hit
ATGGCCCCTGCGCAAGGATGACAAGATCGGAAGAGCACACGTCTGAACTC805070.1538230052395585Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC798690.15260399226748356No Hit
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA796950.15227153418419037Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA793430.15159897530304559Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTG696830.1331418196443558Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC681960.13030064050724696No Hit
CTCGGTTGGCCCCGGATAGCCGGTTCCCCAGATCGGAAGAGCACACGTCT659880.12608186207097502Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC653080.1247826005960362Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT652250.12462401426894809Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA643020.1228604578845826Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC629970.12036702225988383Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGCGTGCATTTATCAGATCAAAACCAACCCGGAGATCGGAAGAGCACAC629260.12023136407647109No Hit
ATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCTGAACTC614640.11743795190535262Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGATTAGCATGGCCCCTGCGCAAGGATGACAAGATCGGAAGAGCACACG605380.1156686634850683No Hit
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC595510.11378282366776737Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGCGTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTC588740.11248929422874741No Hit
GTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACTCCAG580570.11092826978018121Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATCCGTACTATTAACCCCAAGATAAGGGTTTAAGATCGGAAGAGCACAC571890.10926980072099457No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGT571230.10914369593077994No Hit
TCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA568380.1085991525184894Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGTCCCTCCCGTGGTTTCTCTCTCTCCCGGAGATCGGAAGAGCACACGT564400.10783870241992226No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCT559880.10697507567481587Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATCGGAAGAGCACACGTCTG555410.10612100232290755Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC553680.105790454918254No Hit
CTCCATAGCTCAGGGGTTAGAGCACTGGAGATCGGAAGAGCACACGTCTG536390.10248689155035809Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)