FastQCFastQC Report
Tue 19 Sept 2023
SRR16796965_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16796965_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47837298
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAG31741936.635393579294549Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC29198656.103741478040837Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT28064225.866598067474463Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG27964975.8458506582039815TruSeq Adapter, Index 8 (100% over 49bp)
CTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC22428384.688471326286029Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA20757634.33921456015346Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA13550142.8325471058168876Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGT10393672.1727125976053245TruSeq Adapter, Index 8 (100% over 50bp)
AGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCC9475851.9808497545158172Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCA9136041.9098152240956419Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTC6785161.4183827857501485Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC5883481.22989387903974TruSeq Adapter, Index 5 (100% over 35bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCG5831021.2189275405981332TruSeq Adapter, Index 8 (100% over 50bp)
CAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT2574530.5381846608476926TruSeq Adapter, Index 8 (100% over 36bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTC2393850.5004149690896003TruSeq Adapter, Index 8 (100% over 50bp)
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT2284060.47746425811926085Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGTTCTACAGTCCGACGATCCGGAAGAGCACACGTCTGAACTCCAGTCAC2047050.4279192357394433Illumina Multiplexing Index Sequencing Primer (96% over 31bp)
AGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT1847150.38613175852866943TruSeq Adapter, Index 8 (100% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCT1753550.36656543603277925TruSeq Adapter, Index 8 (100% over 50bp)
GTTCTACAGTCCGACGATCATCGGAAGAGCACACGTCTGAACTCCAGTCA1741610.3640694756631112Illumina Multiplexing Index Sequencing Primer (96% over 32bp)
ACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1646240.344133148991818Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1619410.3385245546268102Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATCGGAAGAGCACACGTCAGATCGGAAGAGCACACGTCTGAACTCCAG1514120.3165145322380039Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTTCTACAGTCCGACGATCCGGAAGAGCACACGTCTGAACTCCAGTCACA1293450.2703852546186869TruSeq Adapter, Index 1 (96% over 32bp)
AGATCGGAAGAGCACACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1229270.25696894502695367Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTCTACAGTCCGACGATCGATCGGAAGAGCACACGTCTGAACTCCAGTCA1152950.24101486668415095Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
ATTCGAACGTCTGCCCTATCAGATCGGAAGAGCACACGTCTGAACTCCAG1140920.23850009254285223Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTTCTACAGTCCGACGATCGATCGGAAGAGCACACGTCTGAACTCCAGTC1133570.23696363452634803Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGTTCTACAGTCCGACGATCTCGGAAGAGCACACGTCTGAACTCCAGTCA1018560.21292172480143007Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGTTCTACAGTCCGACGATCATCGGAAGAGCACACGTCTGAACTCCAGTC1004080.20989479798796332Illumina Multiplexing Index Sequencing Primer (96% over 31bp)
AGAGTTCTACAGTCCGACGATCCGGAAGAGCACACGTCTGAACTCCAGTC995460.2080928567495597Illumina Multiplexing Index Sequencing Primer (96% over 29bp)
ACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC931590.1947413501489988Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGAGTTCTACAGTCCGACGATCATCGGAAGAGCACACGTCTGAACTCCAG903840.1889404372295442Illumina Multiplexing Index Sequencing Primer (96% over 29bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT886120.18523621463737353Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT874720.1828531368974895Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGTTCTACAGTCCGACGATCGGAAGAGCACACGTCTGAACTCCAGTCACA846100.17687035751893845TruSeq Adapter, Index 1 (100% over 34bp)
GTTCTACAGTCCGACGATCTCGGAAGAGCACACGTCTGAACTCCAGTCAC744870.15570904527258206Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC723900.15132543648263747TruSeq Adapter, Index 8 (100% over 50bp)
AGAGTTCTACAGTCCGACGATCGATCGGAAGAGCACACGTCTGAACTCCA701920.1467306953666154Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC701360.14661363189869125TruSeq Adapter, Index 8 (100% over 50bp)
TTCTACAGTCCGACGATCATCGGAAGAGCACACGTCTGAACTCCAGTCAC695730.14543672596223975Illumina Multiplexing Index Sequencing Primer (96% over 33bp)
TCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTCCAG690650.1443747930746423Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCTACAGTCCGACGATCCGGAAGAGCACACGTCTGAACTCCAGTCACAC677800.14168860456959756TruSeq Adapter, Index 5 (96% over 33bp)
TCTACAGTCCGACGATCCGGAAGAGCACACGTCTGAACTCCAGTCACACT673030.1406914746731724TruSeq Adapter, Index 8 (97% over 34bp)
ATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGT636370.13302799836228207Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGTTCTACAGTCCGACGATCGATCGGAAGAGCACACGTCTGAACTCCAGT622860.13020384219861247Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
CAGAGTTCTACAGTCCGACGATCCGGAAGAGCACACGTCTGAACTCCAGT582310.12172719286946349Illumina Multiplexing Index Sequencing Primer (96% over 28bp)
AGAGTTCTACAGTCCGACGATCTCGGAAGAGCACACGTCTGAACTCCAGT541750.11324845312124443Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCTAGGAATAATGGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC535150.11186877653499576Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GAGTTCTACAGTCCGACGATCCGGAAGAGCACACGTCTGAACTCCAGTCA534680.1117705268387023Illumina Multiplexing Index Sequencing Primer (96% over 30bp)
AATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC528040.11038248857617335Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCGGAAGAGCACACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA519600.10861817488103112Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT502070.10495367025119187Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCA499940.10450841098926615Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)