FastQCFastQC Report
Tue 19 Sept 2023
SRR16796966_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16796966_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76161364
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT32912994.32148116464931Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG21376252.806705247558329TruSeq Adapter, Index 12 (100% over 49bp)
AGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAG18676282.4521987290038556Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC17255442.26564219621907Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT16717682.1950342170867634Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC13476651.7694864288407441No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGT9684521.271579117201735TruSeq Adapter, Index 12 (100% over 50bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA9232781.212265578646937Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG8857921.1630463971207239Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA8050211.056993937240935Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC6446890.8464777495319018Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG5742560.7539991011715599Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCC5374010.7056084237146802Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCA5373030.7054797495486032Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT4892520.642388705118254Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT4669850.6131520963831478Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA4195990.5509341980797507Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTC4114670.5402568682987348TruSeq Adapter, Index 12 (100% over 50bp)
CAGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTC3827770.5025868496787951Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC3129790.4109419573945655Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA2990500.3926531567895764Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC2902790.3811368189256694Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG2812500.3692817266245389Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCAGGGGTTAGAGCACTGGAGATCGGAAGAGCACACGTCTGAACTCCAG2544730.3341234802464935Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA2480240.3256559323175987Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC2230750.29289785303740096Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTGGTCCGATGGTAGTGAGTTATCTAGATCGGAAGAGCACACGTCTGAA2119720.27831959522153515Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGA2117530.27803204785040353Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC1996520.262143414343262Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1981880.2602211798622724No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT1888930.248016829110361No Hit
GAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCT1698280.22298445180157228TruSeq Adapter, Index 12 (100% over 50bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1666120.21876183835152951No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA1654180.21719411432809949Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGTTAGTGGGGAGATCGGAAGAGCACACGTCTGA1621780.21293998883738477Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCTCCATAGCTCAGGGGTTAGAGCACTGGAGATCGGAAGAGCACACGTC1536780.20177947443273206No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCG1402900.18420100774455667TruSeq Adapter, Index 12 (100% over 50bp)
GCTGGTCCGATGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCT1322030.1735827630398006Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1102960.14481883491477385Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGT1073180.14090871586806142No Hit
AGATCGGAAGAGCACACGTCAGATCGGAAGAGCACACGTCTGAACTCCAG1066470.14002769173094115Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA1049350.13777983282967463Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAA1017290.13357034939657855Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGGGAAGGTGGCTCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA1001060.1314393476461372Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCCCGGGGCTACGCCTGTCTGAGAGATCGGAAGAGCACACGTCTGAACT985100.12934379694145184Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACG932580.12244791204107112No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC908310.1192612569281191No Hit
TATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAG899090.11805066936563793Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACTCCAG874940.11487977027302189Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATTCGAACGTCTGCCCTATCAGATCGGAAGAGCACACGTCTGAACTCCAG863850.11342365139363837Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTTCTACAGTCCGACGATCATCGGAAGAGCACACGTCTGAACTCCAGTCA859910.11290632872594036Illumina Multiplexing Index Sequencing Primer (96% over 32bp)
GCTGGTCCGATGGTAGTGAGTTATCTCAGATCGGAAGAGCACACGTCTGA858740.11275270752766454Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTGAACTCCA838950.11015427717392247Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACTGACCCGGTGAGGCGGGAAGATCGGAAGAGCACACGTCTGAACTCCAG821840.10790773127435059Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC820230.10769633800150953Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCCGGCTCGAAGGACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC813520.10681531386438929Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGATAACTGTGGTAATTCTAGAGCTAATACAAGATCGGAAGAGCACACG805710.1057898595408559No Hit
ACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAAC796510.1045818979817641Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT791420.10391358011917959No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGTC784520.1030076089498607No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)