FastQCFastQC Report
Tue 19 Sept 2023
SRR16796967_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16796967_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64629854
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC19035262.9452735573253808Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT15009092.322315318861776No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT13494692.087996361557617Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC10806831.6721111577940435Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTCCCTCCCGTGGTTTCTCTCTCTCCCGGAGATCGGAAGAGCACACGTC10475961.6209165504226575No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT8650001.3383907690709003Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC8165291.2633929205534025Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT7738501.1973568747347008Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCAGGGGTTAGAGCACTGGAGATCGGAAGAGCACACGTCTGAACTCCAG6663411.0310111485011244Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG6332170.9797592920448187Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCCCTCCCGTGGTTTCTCTCTCTCCCGGAGATCGGAAGAGCACACGTCT4580330.7087018949478054Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTATG4579930.7086400040451895TruSeq Adapter, Index 9 (97% over 36bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA4043490.6256381145468779Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTCCA3882310.6006991753377626Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC3502590.5419461414843982No Hit
GTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC3418320.5289072755757734Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT3313560.5126980481806441No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC3247660.5025015219746589No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGTCTG3196110.49452533190002257Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC3181090.49220132850679194No Hit
GTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTCTGAACTCCA3065230.4742746285640689Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC2789250.4315730003041629No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGT2759030.42689714261152434No Hit
TCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTCCAG2448870.37890693672308157Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT2409980.3728895937162414No Hit
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT2276530.35224124133097995Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC2269330.35112720508389206No Hit
GGCTCCATAGCTCAGGGGTTAGAGCACTGGAGATCGGAAGAGCACACGTC2254290.3488001071455306No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG2198470.3401632316854684Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG2155210.33346973056754853Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC2124330.3286917528855937No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA2107630.3261078077013759Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT2038340.3153867560957201Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA1860520.28787315533777935Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAAC1830370.2832081285530987Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT1771590.2741132604136782Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAG1734110.2683140828385594Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1692870.2619331307788503Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTGAACTCCA1617570.2502821683613891Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG1605900.24847650127756749Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTCCCGTGGTTTCTCTCTCTCCCGGAGATCGGAAGAGCACACGTCTG1586120.2454159961432065Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT1546710.23931819496296555Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA1537320.2378653060240551Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTATGCCGT1484330.2296663087000011RNA PCR Primer, Index 44 (97% over 34bp)
TGTCCCTCCCGTGGTTTCTCTCTCTCCCGGTAGATCGGAAGAGCACACGT1399400.21652532280205988No Hit
GTCTAGGGGTATGATTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCA1360340.2104816761616079Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGTCTGAACTCC1330330.20583831119284288Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA1294750.2003331154051501Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAGGGGTATGATTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAG1290110.19961518093480454Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGTCTG1266460.1959558813176338Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGA1264970.1957253377053892Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATCGGAAGAGCACACGTCTG1258620.1947428196263603Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC1209910.18720605496029746Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGTCTGAACTCCA1168610.18081581926519594Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCT1136060.1757794470648193Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGTCCCTCCCGTGGTTTCTCTCTCTCCCGGAGATCGGAAGAGCACACGT1131730.17510947804400115No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC1105490.17104943483239185No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC1082300.16746130975322954Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGTCGGTTTCTATCTATTTACGATTTCTCCAGATCGGAAGAGCACACGT1081860.16739322976035192No Hit
ACGGGGGAAGGTGGCTCGGGGGGGGAGATCGGAAGAGCACACGTCTGAAC1050470.1625363411775617Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAA1015790.1571703999207549Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGAGATCGGAAGAGCACACGTCT973590.15064090969476737Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATACCCTTTATGAAATTAAAGGACAGATCGGAAGAGCACACGTCTGA958990.14838189174928354Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC942520.14583353383406994Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT938610.14522855026099857No Hit
GCGCAGCGGAAGCGTGCTGGGAGATCGGAAGAGCACACGTCTGAACTCCA914060.14142999611294185Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCGTATAGTGGTTAGTACTAGATCGGAAGAGCACACGTCTGAACTCCAG909330.14069813618950772Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTCTAGGGGTATGATTCTCGGAGATCGGAAGAGCACACGTCTGAACTCCA895940.13862633822443726Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACTCCAG892170.1380430164672815Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA866960.1341423423299084Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC852090.13184154802515877No Hit
GTCCCTCCCGTGGTTTCTCTCTCTCCCGGTAGATCGGAAGAGCACACGTC836570.12944018100365814No Hit
CGGGGGAAGGTGGCTCGGGGGGGGAGATCGGAAGAGCACACGTCTGAACT833920.12903015377382718Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGT817370.12646941767809036No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGA804870.12453532697134052Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC800890.12391951249031137Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTCTGAACTCCAG786030.12162026545812714Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT772510.1195283529497065No Hit
GTCTAGCGGTTAGGATTCCTGAGATCGGAAGAGCACACGTCTGAACTCCA768080.11884291120323434Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGT753870.1166442368878011No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA753800.11663340597984331Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC741860.11478596253675585Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAAGATCGGAAGAGCACACGTCT721880.11169451195108687Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGTCTGAACTC711850.1101425975679908Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGCGTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTGAA710730.10996930304066602Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCTGAA670290.10371213278618886Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTCTGAACTCCAG654220.10122566577359127Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)