FastQCFastQC Report
Tue 19 Sept 2023
SRR16796968_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16796968_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63411853
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT25611554.038921556195495Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC20355203.2099992409936355Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC16925962.669210754651816Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCAGGGGTTAGAGCACTGGAGATCGGAAGAGCACACGTCTGAACTCCAG13280262.094286694318805Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC12102531.9085595874323371Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT11784311.8583765404237598Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG8095331.2766272576831967TruSeq Adapter, Index 7 (100% over 49bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG7349741.1590482933845192Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCCGAGTCACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA6324060.9972993534820691TruSeq Adapter, Index 7 (100% over 35bp)
TACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC6075980.9581773300332352Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT5337580.8417322231539268Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCGGCTCGAAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA4976670.7848169962798595TruSeq Adapter, Index 7 (100% over 35bp)
GTCCCTCCCGTGGTTTCTCTCTCTCCCGGAGATCGGAAGAGCACACGTCT4902290.7730873280110582Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4798700.7567512654140544Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT4720180.7443687223585786Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA4708100.7424637157346593TruSeq Adapter, Index 7 (100% over 35bp)
TTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC4513710.7118085636134935Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGTCCCTCCCGTGGTTTCTCTCTCTCCCGGAGATCGGAAGAGCACACGTC4456380.7027676671110683No Hit
TTCCCGGGCGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG4245620.6695309786957337TruSeq Adapter, Index 7 (100% over 36bp)
TCCAGCGATCCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4083020.6438890849002631Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA4068310.6415693293176592Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAG3817020.6019410913603171Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCCCGGTCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG3719870.5866206117648068TruSeq Adapter, Index 7 (100% over 36bp)
GTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG3500470.5520214020555432TruSeq Adapter, Index 7 (100% over 36bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT3491460.5506005320487953No Hit
GTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT3167000.4994334418834914Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3104190.48952835363445374Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA3051030.481145062895418TruSeq Adapter, Index 7 (100% over 37bp)
CTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAACTCCA2973890.46898014476883365Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC2952410.4655927654408711Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2886810.45524769635733564Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT2837020.44739585200262166Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA2769290.4367148835723189TruSeq Adapter, Index 7 (100% over 35bp)
TCAGAAGGTTGCGTGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC2726330.42994012491639383Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC2404800.3792350934769246Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGCGTGCATTTATCAGATCAAAACCAACCCGGAGATCGGAAGAGCACACG2220380.35015220261738766No Hit
GTCTAGCGGTTAGGATTCCTGAGATCGGAAGAGCACACGTCTGAACTCCA2143860.33808505800958066Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG2019940.3185429701920239TruSeq Adapter, Index 7 (100% over 36bp)
GTGCATTTATCAGATCAAAACCAACCCGGAGATCGGAAGAGCACACGTCT1942880.3063906680033463Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA1880790.29659912319546944TruSeq Adapter, Index 7 (100% over 37bp)
TGCGTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCT1851570.2919911518750288Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTGAACTCCA1837640.28979440168701587Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA1834350.2892755712406007TruSeq Adapter, Index 7 (100% over 35bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT1743870.2750069454680657Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCGGCTCGAAGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1722380.27161798914786484Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1695650.2674026888947718Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGT1648280.259932476661737TruSeq Adapter, Index 7 (100% over 50bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC1627490.2566539097982202No Hit
GTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTCTGAACTCCA1586760.2502308204114458Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1565810.24692702167211544No Hit
GTGCATTTATCAGATCAAAACCAACCCGGTAGATCGGAAGAGCACACGTC1471100.23199132818276105No Hit
AATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1463930.2308606247478685Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC1453770.22925840063371117No Hit
CAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG1412060.22268076600757905TruSeq Adapter, Index 7 (100% over 36bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG1370700.21615832610978897Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACTCCAG1332480.21013106177483884Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA1296450.204449158740086TruSeq Adapter, Index 7 (100% over 37bp)
ATCCCAGCGGTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA1295390.2042819975628216TruSeq Adapter, Index 7 (100% over 35bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC1292060.2037568591474531No Hit
GCGCAATGGATAGCGCATTGGAGATCGGAAGAGCACACGTCTGAACTCCA1282520.20225240855207305Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC1246250.1965326577036No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGGAGATCGGAAGAGCACAC1242490.1959397086219827No Hit
CTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTCTGAACTCCAG1204120.1898887894034574Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGCGTGCATTTATCAGATCAAAACCAACCCGGTAGATCGGAAGAGCACAC1194070.18830391220392187No Hit
ATGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT1192890.1881178271197973Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGCGTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTC1191220.18785446941599387No Hit
GTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACTCCA1187160.18721421056722629Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGGGTTAGAGCACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1164190.1835918593957505Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAAC1155260.18218360532690947Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1151490.18158907925305384No Hit
GGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC1080370.17037351045395252Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACCATCCTCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA1066910.1682508789011417TruSeq Adapter, Index 7 (100% over 37bp)
AGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCC1052950.16604939773641372Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTCCATAGCTCAGGGGTTAGAGCACTGGAGATCGGAAGAGCACACGTC1031910.1627314060669383No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCG1027710.1620690693268339TruSeq Adapter, Index 7 (100% over 50bp)
CTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGTCTGAACTCCA932420.14704191028765554Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCA902760.14236455130872772Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCCGGCTCGAAGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA896020.14130165854008397Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGT872830.13764461353936464Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG847320.13362170633934953Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC846470.13348766199909032Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCTGAACTC834070.13153219162354396Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTAGTATAGTGGTTAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACT831100.13106382492875582Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCT829560.13082096812405086Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT828520.1306569609312631TruSeq Adapter, Index 7 (100% over 38bp)
TCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTCC817470.1289143845078932Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCCAGCGATCCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC817100.12885603579507446Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT798840.12597644796785865No Hit
GTCTAGCGGTTAGGATTCCTAGATCGGAAGAGCACACGTCTGAACTCCAG798270.12588655941027302Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATAGCTCAGGGGTTAGAGCACTGGAGATCGGAAGAGCACACGTCTGAACT753840.11887998289531138Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT750860.11841003920828493Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCGGCTCGAAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC749480.11819241427939346Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT744850.11746226687304029Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG735100.11592469944065505Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA733720.11570707451176361Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTC733630.1156928815816185Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAACTCCAG732190.11546579469929698Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTAGTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACT731330.11533017336679942Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCA726230.11452590732524406Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGTCGGTTTCTATCTATTTACGATTTCTCCAGATCGGAAGAGCACACGT726050.11449752146495387No Hit
GTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGTCTGAACTCC716040.11291895223437169Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACT696790.10988324217555982Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATCCGTACTATTAACCCCAAGATAAGGGTTTAAGATCGGAAGAGCACAC677980.10691691977523507No Hit
TATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAG672910.10611738471039477Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA670720.10577202341019747Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC652930.10296655421818378No Hit
GGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA652870.10295709226475372TruSeq Adapter, Index 7 (100% over 35bp)
TTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC648640.10229002454793427Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)