FastQCFastQC Report
Tue 19 Sept 2023
SRR16796969_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16796969_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88349149
Total Bases4.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT37546034.249733067604307Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA17821612.017179588226707No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC10798881.222295870671035Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC10049471.1374721900264144No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT8564080.9693449339279996Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA8223680.9308159832982659Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG8062430.9125645341530115Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC7168630.8113977419295799Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT6105070.6910162767951505No Hit
TTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC5875840.6650703562521015Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAG5818070.6585315269986358Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG5731420.6487238490548449Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT5509100.6235600526271057Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC4798640.5431450165977264Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT4521190.5117412053397368Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC4444900.5031061476325029No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG4332990.49043935895749263TruSeq Adapter, Index 2 (100% over 49bp)
CTCAGGGGTTAGAGCACTGGAGATCGGAAGAGCACACGTCTGAACTCCAG3825950.4330488797351064Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCCGGCTCGAAGGACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC3693240.41802779560446024Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACTCCAG3634640.4113950209073321Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC3491210.3951605691187812No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA3259110.36888980107776703Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG3225190.3650504884885762Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3064380.34684884174719105Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3053440.34561057288735175Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA2981850.3375074954032664Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT2780210.3146844119573806No Hit
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC2562370.29002769455085525Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACAC2472040.2798034874110672No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC2466020.27912209997631104No Hit
ATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGT2439020.27606604337524515Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACG2319180.26250167955777365No Hit
GTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGA2194720.2484143904996753Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT2174030.24607254564500675No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC2149590.2433062484846345No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC2127880.24084895260281453No Hit
GTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC2059110.2330650632526183Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCCGGCTCGAAGGACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC2005820.2270333130203665Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1934290.21893702677317242No Hit
GTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTGAACTCCA1932240.21870499284605444Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA1785600.20210720988382128Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCC1662580.18818291051111313Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCA1652110.1869978396735887Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA1610680.18230849060017545Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG1601960.1813214975053127Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGT1587880.1797278205814976TruSeq Adapter, Index 2 (100% over 50bp)
CGGGGGAAGGTGGCTCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA1554830.17598698092722997Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTCCA1508400.17073169544621195Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTCC1501010.16989524143577206Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1432210.1621079564671302No Hit
TACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC1419190.16063425806172735Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCCA1400830.15855613957300257Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT1372850.1553891594360462Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1349900.1527915113251402Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGGTTGGCCCCGGATAGCCGGTTCCCAGATCGGAAGAGCACACGTCTG1320300.14944116779211988Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATAGCTAAAGACACCTTGCCTAGCCACGCAGATCGGAAGAGCACACGTC1305870.14780787531977246No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT1289170.14591764771837248Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCTCCATAGCTCAGGGGTTAGAGCACTGGAGATCGGAAGAGCACACGTC1229620.13917734510379948No Hit
CAGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTC1175570.13305957253759174Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC1145790.12968885529389762No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT1142490.12931533726487845No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC1129660.12786314444296457No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT1111500.125807663410544Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT1110930.12574314666007705Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA1109050.12553035457081765Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGT1100790.12459542762545456No Hit
AAGATTAGCATGGCCCCTGCGCAAGGATGACAAGATCGGAAGAGCACACG1084050.12270067253279371No Hit
GTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTCTGAACTCCA1072440.12138656819433542Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC1057370.11968083586181458No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACA1057040.11964348405891266No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT1019990.1154498952785612Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTAGGAATAATGGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1015950.11499261866121653Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGGTCGGTTTCTATCTATTTACGATTTCTCAGATCGGAAGAGCACACGTC1005360.11379396534990958No Hit
GCGCAGCGGAAGCGTGCTGGGAGATCGGAAGAGCACACGTCTGAACTCCA989390.11198636446401991Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC958030.10843681131552267Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGAATGAGTCCACTTTAAATCCTTTAACGAGGAGATCGGAAGAGCACAC944770.1069359479625548No Hit
ACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAA919300.10405306790221601Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGACTGCATAATTTGTGTTAGTGGGGAGATCGGAAGAGCACACGTCTGA913150.10335696612086213Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTGGTCCGATGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCT906950.1026552049754322Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC896860.10151314530488574No Hit
TTTCCGGCTCGAAGGACCAAGATCGGAAGAGCACACGTCTGAACTCCAGT890040.10074120804491281Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTCTAGCGGTTAGGATTCCTGAGATCGGAAGAGCACACGTCTGAACTCCA889120.10063707574591353Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC885230.10019677722079699Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)