FastQCFastQC Report
Tue 19 Sept 2023
SRR16796970_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16796970_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75106201
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT26958713.589412011399698Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC10522951.401076057621394No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA10303221.3718201510418562Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG9723821.2946760547774212Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG6056660.8064127754244952TruSeq Adapter, Index 10 (100% over 49bp)
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC5337210.7106217501268637Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT5294130.7048858722064774Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG5213380.694134429725716Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA5188540.6908271129303957Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT3967570.5282613082773285Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA3904430.5198545456985636Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG3108340.41385930304210167Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA3041980.4050238142120915Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC2943180.3918691081179835Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAG2834190.37735765652692244Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCCCGAGGTGGGATCCCGAGGCCAGATCGGAAGAGCACACGTCTGAACT2533480.33731968416296276Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC2152640.28661281909332625Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGA1926520.256506117251224Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC1798910.23951550951165804Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT1798730.23949154344792384Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGT1794740.23896029570181562TruSeq Adapter, Index 10 (100% over 50bp)
GCTGGTCCGATGGTAGTGAGTTATCTAGATCGGAAGAGCACACGTCTGAA1780690.23708961128256242Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGCCCGAGGTGGGATCCCGAGGCCTAGATCGGAAGAGCACACGTCTGAA1521660.20260111412105639Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA1498110.19946555411583128Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGTTAGTGGGGAGATCGGAAGAGCACACGTCTGA1467800.1954299352725882Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1404080.18694594871067968Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGCCCGAGGTGGGATCCCGAGGCCTAGATCGGAAGAGCACACGTCTGA1233860.1642820411060333Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1224520.16303846868782512Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCCCGAGGTGGGATCCCGAGGCCAGATCGGAAGAGCACACGTCTGAAC1185790.15788177064101538Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACGTCTGAA1171940.15603771518146684Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGGGAAGGTGGCTCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA1136450.15131240628187279Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGTCGGTTTCTATCTATTTACGATTTCTCAGATCGGAAGAGCACACGTC1135440.1511779300353642No Hit
GGGCCCGAGGTGGGATCCCGAGGCAGATCGGAAGAGCACACGTCTGAACT1111800.14803038699827195Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGATAACTGTGGTAATTCTAGAGCTAATACAAGATCGGAAGAGCACACG1098360.14624092090611798No Hit
TTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1082200.1440893009619805Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA1056610.1406821255677677Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAAC1043350.13891662553934794Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA942400.1254756581284147Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC935730.12458758232226391Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAA916090.12197261847926512Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACCTGGTATTGCAGTACTTCCAGGAGATCGGAAGAGCACACGTCTGAAC893270.11893425417696205Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCC880190.11719272021227649Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC836090.11132103459739628Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCA826130.10999491240410361Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAAACCGAAGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCT800060.10652382750659962Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAACCGAAGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTC790400.10523764875286397No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)