FastQCFastQC Report
Tue 19 Sept 2023
SRR16913146_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16913146_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61863562
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAAAAGGGGTTGTAGGACATCAGATCGGAAGAGCACACGTCTGAACTCC6089540.9843500443766883Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGT6029040.9745704587783032No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCT5156640.8335504509100203Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGACGAAGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT4304350.6957811449654322TruSeq Adapter, Index 2 (100% over 37bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT4086130.6605067454732076No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT3310560.5351389239436294No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGAGATCGGAAGAGCAC3188640.5154310383873466No Hit
AGGACCGACTGACCCATGTTCAGATCGGAAGAGCACACGTCTGAACTCCA2998680.48472475606884713Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTAATTTTTTATCAACCTGTACCGAGAACGAGATCGGAAGAGCACACGTC2978090.48139646404453723No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC2625880.42446311125764147No Hit
ACCGGAGTGGACGTACCGCTGGTGTTCCAGTTGAGATCGGAAGAGCACAC2346820.37935416651242937No Hit
ATAGGAAGATAGGAAGTTGCTTTAGATCGGAAGAGCACACGTCTGAACTC1904460.30784842295372516Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGAATTTTTGAGCGGGTAAAGGTCGCCCTCAAGAGATCGGAAGAGCACA1825820.2951365781362541No Hit
ACGGTCTGTATTCGTACTGAAAATCAGATCGGAAGAGCACACGTCTGAAC1793330.28988469820085694Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT1773790.286726134521643Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG1750110.28289835622462217Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC1451220.2345839704477411No Hit
CTTAGTTGAACAGGGCCCTGAAGCGAGATCGGAAGAGCACACGTCTGAAC1351130.21840481800902445Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAGGAAAAGACTCATATCAACATTGAGATCGGAAGAGCACACGTCTGAA1324090.21403390900769664Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTC1319540.2132984195122809No Hit
CATAATTTGTGGTAGTGGGGGACTGAGATCGGAAGAGCACACGTCTGAAC1311510.21200040178740434Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTC1300050.21014793813521437TruSeq Adapter, Index 2 (100% over 50bp)
ACGGGCTGGGCCTCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1237880.20009840364510534Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT1225310.19806651288524252TruSeq Adapter, Index 2 (100% over 43bp)
AGGACGAAGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT1194820.19313792503574237TruSeq Adapter, Index 2 (100% over 37bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC1101230.17800947187619104No Hit
TAAAAAGGGGTTGTAGGACATTAGATCGGAAGAGCACACGTCTGAACTCC1002000.16196933503441008Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGCTAGTTGATTCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC987040.15955111023190033Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AACGCTCGCTGTGTGCCTAATACATGAGATCGGAAGAGCACACGTCTGAA922750.1491588861307404Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCA863060.13951023382714367No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC850100.13741530111053096No Hit
TAAAAAGGGGTTGTAGGACATAGATCGGAAGAGCACACGTCTGAACTCCA835330.13502778905618143Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCAGGATGAACGCTCGCTGTGTGAGATCGGAAGAGCACACGTCTGAACT798700.12910669450297738Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTAAAACTCAAAGGACCTGGCGGTGAGATCGGAAGAGCACACGTCTGAA798510.12907598175481716Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACTCTAGATCGGAAGAGCACACGTCTG787550.12730434112410144Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCGTGCTGTAAGACCTAAAGTTCTTATGAGATCGGAAGAGCACACGTCT766310.12387097917187503Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGTGCTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA737090.1191476817969195TruSeq Adapter, Index 2 (100% over 36bp)
AAAGGGAGCAGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC737000.11913313365305411Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG705250.11400087178943884Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTCTCTCGGAAGTAGAACGCAACCTCCAGAGATCGGAAGAGCACACGTC645560.10435221948584208No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTGA638150.10315442230759361Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGAGAACTCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG630220.1018725691870119TruSeq Adapter, Index 2 (100% over 35bp)
TGCATGGTTGTCGTCAGCTCGTGAGATCGGAAGAGCACACGTCTGAACTC629340.10173032066921721Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATAGGAAGATAGGAAGTTGCTTTTAGATCGGAAGAGCACACGTCTGAACT626220.10122598501521785Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)