FastQCFastQC Report
Tue 19 Sept 2023
SRR16913147_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16913147_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37731206
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAAAAGGGGTTGTAGGACATCAGATCGGAAGAGCACACGTCTGAACTCC4093451.0848977369024464Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGACGAAGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG3917891.0383686119123783TruSeq Adapter, Index 5 (100% over 37bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCT3031110.8033429941253402Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGACCGACTGACCCATGTTCAGATCGGAAGAGCACACGTCTGAACTCCA2438100.6461760061419717Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAAAAAGGGGTTGTAGGACATAGATCGGAAGAGCACACGTCTGAACTCCA1941010.5144309460980389Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCGGAGTGGACGTACCGCTGGTGTTCCAGTTGAGATCGGAAGAGCACAC1899770.5035010012666968No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGT1781380.4721237905833172No Hit
AGGACGAAGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG1740540.4612998587959261TruSeq Adapter, Index 5 (100% over 37bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGAGATCGGAAGAGCAC1529210.4052905173505453No Hit
ATAGGAAGATAGGAAGTTGCTTTAGATCGGAAGAGCACACGTCTGAACTC1428990.3787289491886371Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT1329640.3523979593973222No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG1307090.34642147404458795Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1203310.3189163897915163No Hit
ACGGTCTGTATTCGTACTGAAAATCAGATCGGAAGAGCACACGTCTGAAC1144490.30332717167853046Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAGGAAAAGACTCATATCAACATTGAGATCGGAAGAGCACACGTCTGAA1140940.3023863059134659Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC1063150.2817694191911067No Hit
TTAATTTTTTATCAACCTGTACCGAGAACGAGATCGGAAGAGCACACGTC935260.24787439871389216No Hit
TAAAAAGGGGTTGTAGGACATTAGATCGGAAGAGCACACGTCTGAACTCC899720.23845513975885108Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT864840.22921080232632904TruSeq Adapter, Index 5 (100% over 43bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT847750.2246813950235251Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATGGTTGTCGTCAGCTCGTGTCGTGAGATGAGATCGGAAGAGCACACGT758520.20103253524416898No Hit
ACGGTGCTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA666300.1765912279612796TruSeq Adapter, Index 5 (100% over 36bp)
CTTAGTTGAACAGGGCCCTGAAGCGAGATCGGAAGAGCACACGTCTGAAC605390.1604480916936501Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACACATTGATCATCGACACTTCGAACGAGATCGGAAGAGCACACGTCTGA601450.15940386321073333Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGAGAACTCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC592330.15698676580865187TruSeq Adapter, Index 5 (100% over 35bp)
ACGGGCTGGGCCTCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC590770.1565733149372432Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTTAAAACTCAAAGGACCTGGCGGTGAGATCGGAAGAGCACACGTCTGAA587090.15559799493289453Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACTCTAGATCGGAAGAGCACACGTCTG575770.15259782578908293Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCA572990.1518610351336239No Hit
AAAGGGAGCAGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC571640.15150324111028945Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC559760.14835465370494652No Hit
TTCGTGCTGTAAGACCTAAAGTTCTTATGAGATCGGAAGAGCACACGTCT558060.147904098268155Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGACCGACTGACCCATGTTTAGATCGGAAGAGCACACGTCTGAACTCCA554850.14705334359044872Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CATAATTTGTGGTAGTGGGGGACTGAGATCGGAAGAGCACACGTCTGAAC551860.14626089608691542Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGGCTAGTTGATTCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC532940.14124647910803595Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AACCAATGTTAAGGAACTCTGCAAAATAACCCCGAGATCGGAAGAGCACA530600.14062630280092292No Hit
AACGCTCGCTGTGTGCCTAATACATGAGATCGGAAGAGCACACGTCTGAA522850.13857230007437343Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATAGGAAGATAGGAAGTTGCTTTTAGATCGGAAGAGCACACGTCTGAACT507710.13455970636083034Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGGAAACTCGACTGCATAATTTGAGATCGGAAGAGCACACGTCTGAACT504180.1336241412479633Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCACAAGAACTGCTAACTCATGAGATCGGAAGAGCACACGTCTGAACTC495580.13134486080301808Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC486690.12898872090120841No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC476990.1264179045853981No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTGA469780.12450701946818239Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTGTTTAATGGGGGTAGAGCACTGAATGAGATCGGAAGAGCACACGTCT466890.12374107522563683Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCATGGTTGTCGTCAGCTCGTGAGATCGGAAGAGCACACGTCTGAACTC452890.12003061868735392Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAAGACTGACGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC443100.11743594943665464TruSeq Adapter, Index 5 (100% over 35bp)
CTTAAAACTCAAAGGACCTGGCGAGATCGGAAGAGCACACGTCTGAACTC434750.11522292714417876Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGGAATCGAGAAAGAGCTATCAGATCGGAAGAGCACACGTCTGAACTCC429780.1139057150730883Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGACGAAGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG412230.10925439276974079TruSeq Adapter, Index 5 (100% over 37bp)
CATGGGCCAGAAGGACTCCTACGTGAGATCGGAAGAGCACACGTCTGAAC402860.10677103721518999Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAAGGACGTGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG400900.10625157329983037TruSeq Adapter, Index 5 (100% over 37bp)
CTTAAAACTCAAAGGACCTGGCGGAGATCGGAAGAGCACACGTCTGAACT391090.10365160339693358Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGTGATCGTATAGTGGTTAGTACTCTAAGATCGGAAGAGCACACGTCT387570.10271868861016528Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)