FastQCFastQC Report
Tue 19 Sept 2023
SRR16913148_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16913148_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41577634
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTC6090411.4648284219347354TruSeq Adapter, Index 1 (100% over 50bp)
ATAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT5378001.2934838956925736TruSeq Adapter, Index 1 (100% over 43bp)
AGGACGAAGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA4981231.198055185150747TruSeq Adapter, Index 1 (100% over 37bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT3174070.7634080380812435No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG3093030.7439167894931203TruSeq Adapter, Index 1 (100% over 49bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCT2488640.5985525775709123Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCAGGATGAACGCTCGCTGTGTGCCTAATACATGAGATCGGAAGAGCAC2318920.5577325539976613No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1963750.4723092227903108No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC1945110.4678260432039014No Hit
ATAGGAAGATAGGAAGTTGCTTTAGATCGGAAGAGCACACGTCTGAACTC1796930.43218668960335743Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGACGAAGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA1744740.4196342677892638TruSeq Adapter, Index 1 (100% over 37bp)
AAAGGAAAAGACTCATATCAACATTGAGATCGGAAGAGCACACGTCTGAA1521560.36595636971550616Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAAAAAGGGGTTGTAGGACATCAGATCGGAAGAGCACACGTCTGAACTCC1389670.3342349879745442Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGACCGACTGACCCATGTTCAGATCGGAAGAGCACACGTCTGAACTCCA1350590.3248357037343684Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATAGGTGGGAGACTTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1339010.32205055246770414Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGAATTTTTGAGCGGGTAAAGGTCGCCCTCAAGAGATCGGAAGAGCACA1307160.3143901839147461No Hit
ACCGGAGTGGACGTACCGCTGGTGTTCCAGTTGAGATCGGAAGAGCACAC1068890.2570829306929779No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG1007010.24219992893294504Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAAGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA943030.22681184792766226TruSeq Adapter, Index 1 (100% over 40bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT871100.2095116812082188Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAGGGAGCAGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC851380.20476874658139518Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGTGCTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC781240.18789909979004576TruSeq Adapter, Index 1 (100% over 36bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC726030.17462032591849744No Hit
ACGGTCTGTATTCGTACTGAAAATCAGATCGGAAGAGCACACGTCTGAAC721760.1735933314531558Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGAGAACTCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT715390.1720612577425642TruSeq Adapter, Index 1 (100% over 35bp)
TTAATTTTTTATCAACCTGTACCGAGAACGAGATCGGAAGAGCACACGTC709220.17057728681723447No Hit
ACGGGCTGGGCCTCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC692990.16667374579323105Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CATGGTTGTCGTCAGCTCGTGTCGTGAGATGAGATCGGAAGAGCACACGT686460.16510318985442993No Hit
GAAGGACGTGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA684800.16470393673675612TruSeq Adapter, Index 1 (100% over 37bp)
AGGGCTAGTTGATTCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC683580.1644105097466585Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC661010.15898211042985275No Hit
ATAGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT601970.14478216822053896TruSeq Adapter, Index 1 (100% over 43bp)
ATAGGAAGATAGGAAGTTGCTTTTAGATCGGAAGAGCACACGTCTGAACT563830.13560896707109404Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGTGATCGTATAGTGGTTAGTACTCTAGATCGGAAGAGCACACGTCTG563090.13543098676562498Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAAGACTGACGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT555550.13361751176125125TruSeq Adapter, Index 1 (100% over 35bp)
AACCAATGTTAAGGAACTCTGCAAAATAACCCCGAGATCGGAAGAGCACA548980.13203733526539774No Hit
AACGCTCGCTGTGTGCCTAATACATGAGATCGGAAGAGCACACGTCTGAA545800.13127250097973348Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCGTGCTGTAAGACCTAAAGTTCTTATGAGATCGGAAGAGCACACGTCT538610.12954320584956805Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTAGTTGAACAGGGCCCTGAAGCGAGATCGGAAGAGCACACGTCTGAAC534120.12846329832043835Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCAGGATGAACGCTCGCTGTGTGAGATCGGAAGAGCACACGTCTGAACT532150.1279894858856086Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAT515490.12398252387329207TruSeq Adapter, Index 1 (100% over 41bp)
CATAATTTGTGGTAGTGGGGGACTGAGATCGGAAGAGCACACGTCTGAAC510010.12266450755711593Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC494860.11902072157352678No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAAGATCGGAAGAGCACACGT459450.11050412344290683No Hit
AAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCA436060.10487850270652725No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)