FastQCFastQC Report
Tue 19 Sept 2023
SRR16913149_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16913149_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63946926
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACGAAGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG19122392.990353281407147TruSeq Adapter, Index 3 (100% over 37bp)
TAAAAAGGGGTTGTAGGACATCAGATCGGAAGAGCACACGTCTGAACTCC9148201.4305926136308726Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGACCGACTGACCCATGTTCAGATCGGAAGAGCACACGTCTGAACTCCA7269821.13685214516801Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGGTCTGTATTCGTACTGAAAATCAGATCGGAAGAGCACACGTCTGAAC4497170.7032660178223422Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGACGAAGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG4451020.6960490954639478TruSeq Adapter, Index 3 (100% over 37bp)
ATAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT4156080.649926471836973TruSeq Adapter, Index 3 (100% over 43bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCT3729540.5832242819615754Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT3090510.48329297330101534No Hit
ATAGGAAGATAGGAAGTTGCTTTAGATCGGAAGAGCACACGTCTGAACTC2839760.44408076785426714Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC2721330.4255607220275139No Hit
AAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCA2509290.3924019740995838No Hit
AGGGCTAGTTGATTCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC2436890.38108008506929636Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACCGGAGTGGACGTACCGCTGGTGTTCCAGTTGAGATCGGAAGAGCACAC2411350.377086147972148No Hit
ACGGGCTGGGCCTCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC2381780.3724620007535624Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT2301840.35996100891542465No Hit
AAAGGAAAAGACTCATATCAACATTGAGATCGGAAGAGCACACGTCTGAA2186970.34199767475922144Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTC2079430.3251806036774934TruSeq Adapter, Index 3 (100% over 50bp)
TAAAAAGGGGTTGTAGGACATAGATCGGAAGAGCACACGTCTGAACTCCA1986210.3106028896525847Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTAATTTTTTATCAACCTGTACCGAGAACGAGATCGGAAGAGCACACGTC1683990.2633418219352718No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCA1652480.2584142981321729No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAGATCGGAAGAGCACACGT1625830.25424677958718456No Hit
ACGGTGCTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA1581780.24735825456254143TruSeq Adapter, Index 3 (100% over 36bp)
CATAATTTGTGGTAGTGGGGGACTGAGATCGGAAGAGCACACGTCTGAAC1559100.243811563358026Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT1548840.24220710781312615Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG1474930.2306490854619032Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC1425800.22296615164894715No Hit
AAAGGGAGCAGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1343090.21003198808962295Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGGAATCGAGAAAGAGCTATCAGATCGGAAGAGCACACGTCTGAACTCC1321700.2066870266758405Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAAAAAGGGGTTGTAGGACATTAGATCGGAAGAGCACACGTCTGAACTCC1270250.19864129199893046Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGGC1265660.1979235092551595No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC1235250.1931680031030733No Hit
GAAGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA1206870.18872994770694687TruSeq Adapter, Index 3 (100% over 40bp)
AGGGACTTAATCAACGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1185150.18533338099786062Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAGAGAACTCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT1182200.18487206093378125TruSeq Adapter, Index 3 (100% over 35bp)
AGGACCGACTGACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT1154120.1804809194424764Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAAGGACGTGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG1128900.17653702384380449TruSeq Adapter, Index 3 (100% over 37bp)
ATAGGAAGATAGGAAGTTGCTTTTAGATCGGAAGAGCACACGTCTGAACT1099270.17190349384425455Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGACCGACTGACCCATGTTTAGATCGGAAGAGCACACGTCTGAACTCCA1079380.16879310195458025Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACGCTCGCTGTGTGCCTAATACATGAGATCGGAAGAGCACACGTCTGAA1076910.1684068441382155Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGACGAAGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG1043310.1631524867981926TruSeq Adapter, Index 3 (100% over 37bp)
CAGGACGAAGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA1038500.1624003005242191TruSeq Adapter, Index 3 (100% over 36bp)
GAAGACTGACGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT1005410.157225696822393TruSeq Adapter, Index 3 (100% over 35bp)
CTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTC1001740.15665178338674168No Hit
CTTAGTTGAACAGGGCCCTGAAGCGAGATCGGAAGAGCACACGTCTGAAC930700.14554257072497903Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGGCGATGAAGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC909680.14225546979380993Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATAGGAAGATAGGAAGTTGCTTAGATCGGAAGAGCACACGTCTGAACTCC840670.13146370788800701Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAGGGCAGGGACTTAATCAACGCAGATCGGAAGAGCACACGTCTGAACT829350.12969348987940404Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTCGTACTGAAAATCAAGATCAGATCGGAAGAGCACACGTCTGAACTCC819850.12820788289338567Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGGTCTGTATTCGTACTGAAAATCAAGATCGGAAGAGCACACGTCTGAA811880.12696153682195763Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CATGGTTGTCGTCAGCTCGTGTCGTGAGATGAGATCGGAAGAGCACACGT791830.12382612418304517No Hit
AGGACGAAGGGCACTCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGT781830.1222623273556574Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG736790.11521898644510292TruSeq Adapter, Index 3 (100% over 49bp)
CTCAGGATGAACGCTCGCTGTGTGAGATCGGAAGAGCACACGTCTGAACT732820.11459815910462999Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTAAAACTCAAAGGACCTGGCGGTGAGATCGGAAGAGCACACGTCTGAA683270.10684954582492363Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACTCTAGATCGGAAGAGCACACGTCTG681300.10654147784992823Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGAGGGAGGAAGACGAACAGATCGGAAGAGCACACGTCTGAACTCCAG676890.10585184344905024Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG647690.10128555671307796Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTAAGGACTCTAGAGATACTGCCTGGAGATCGGAAGAGCACACGTCTGAA644940.10085551258554634Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CATAATCTTGAGTTGTGATGCATAGTGAGATCGGAAGAGCACACGTCTGA644510.10078826932196866Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)