FastQCFastQC Report
Tue 19 Sept 2023
SRR16913150_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16913150_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46207923
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAAAAGGGGTTGTAGGACATCAGATCGGAAGAGCACACGTCTGAACTCC9717932.1030873861177444Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGACGAAGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT5206091.1266660914406388TruSeq Adapter, Index 8 (100% over 37bp)
TAAAAAGGGGTTGTAGGACATAGATCGGAAGAGCACACGTCTGAACTCCA5058531.0947321739607296Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCT4410160.9544164103632186TruSeq Adapter, Index 8 (100% over 43bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCT4339600.9391463018149507Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGGAGTGGACGTACCGCTGGTGTTCCAGTTGAGATCGGAAGAGCACAC3707760.8024078468101672No Hit
ACGGTGCTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT2445970.529339957565286TruSeq Adapter, Index 8 (100% over 36bp)
ATAGGAAGATAGGAAGTTGCTTTAGATCGGAAGAGCACACGTCTGAACTC2414810.5225965252755463Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGACCGACTGACCCATGTTCAGATCGGAAGAGCACACGTCTGAACTCCA2265380.49025791529301155Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT2000620.432960382140526No Hit
AAAGGAAAAGACTCATATCAACATTGAGATCGGAAGAGCACACGTCTGAA1961760.4245505689576223Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1750450.378820316160932No Hit
TAAAAAGGGGTTGTAGGACATTAGATCGGAAGAGCACACGTCTGAACTCC1642830.3555299380151754Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC1600550.34637999201998326No Hit
AGGACGAAGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT1579440.3418115114154774TruSeq Adapter, Index 8 (100% over 37bp)
CATGGTTGTCGTCAGCTCGTGTCGTGAGATGAGATCGGAAGAGCACACGT1534540.33209456309040336No Hit
AAAGGGAGCAGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1429680.3094014851089498Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAAGGACGTGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT1413040.3058003710748912TruSeq Adapter, Index 8 (100% over 37bp)
ACACATTGATCATCGACACTTCGAACGAGATCGGAAGAGCACACGTCTGA1251380.27081502884256453Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGAGAACTCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC1150370.24895514130769308TruSeq Adapter, Index 5 (100% over 35bp)
ACGGTCTGTATTCGTACTGAAAATCAGATCGGAAGAGCACACGTCTGAAC1139280.24655511999533067Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT1076310.23292758689889612Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGTTTAATGGGGGTAGAGCACTGAATGAGATCGGAAGAGCACACGTCT1063190.2300882469874268Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAGACTGACGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC1018290.22037129866235278TruSeq Adapter, Index 5 (100% over 35bp)
ATAGGAAGATAGGAAGTTGCTTTTAGATCGGAAGAGCACACGTCTGAACT978180.21169096910068863Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CATAATTTGTGGTAGTGGGGGACTGAGATCGGAAGAGCACACGTCTGAAC970360.2099986186351635Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACCAATGTTAAGGAACTCTGCAAAATAACCCCGAGATCGGAAGAGCACA967440.20936669237437916No Hit
GAAGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAA937050.202789898173956TruSeq Adapter, Index 8 (100% over 40bp)
AACGCTCGCTGTGTGCCTAATACATGAGATCGGAAGAGCACACGTCTGAA925220.20022973116536746Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATAGGAAGATAGGAAGTTGCTTAGATCGGAAGAGCACACGTCTGAACTCC866700.18756523637731998Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCACAAGAACTGCTAACTCATGAGATCGGAAGAGCACACGTCTGAACTC865510.18730770478474007Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTAAAACTCAAAGGACCTGGCGGTGAGATCGGAAGAGCACACGTCTGAA861470.1864333958485864Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG855730.1851911846373186Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGAAACTCGACTGCATAATTTGAGATCGGAAGAGCACACGTCTGAACT806960.17463671760360233Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGTGATCGTATAGTGGTTAGTACTCTAGATCGGAAGAGCACACGTCTG803820.17395718046015615Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC781090.1690381106287768No Hit
TTAATTTTTTATCAACCTGTACCGAGAACGAGATCGGAAGAGCACACGTC739230.1599790581368481No Hit
CATAATCTTGAGTTGTGATGCATAGTGAGATCGGAAGAGCACACGTCTGA726430.15720897041834145Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC716160.1549864078504459No Hit
CTTAGTTGAACAGGGCCCTGAAGCGAGATCGGAAGAGCACACGTCTGAAC713220.1543501533276014Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGGTGCTGAAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT688490.1489982572902054TruSeq Adapter, Index 8 (100% over 36bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTC684840.148208349464225TruSeq Adapter, Index 8 (100% over 50bp)
TAAAACTAAGTGGAGGACCGAACCGTAGTACGAGATCGGAAGAGCACACG660650.14297331650245348No Hit
ATAGGAAGATAGGAAGTTGCTTTATAGGAAGATCGGAAGAGCACACGTCT656420.14205788907672826Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTAAGGACTCTAGAGATACTGCCTGGAGATCGGAAGAGCACACGTCTGAA602910.13047762393475248Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCTG586960.1270258349417696Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGAGGACCGAACCGTAGTACGAGATCGGAAGAGCACACGTCTGAACTCC575020.12444186249185014Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTAAAACTCAAAGGACCTGGCGAGATCGGAAGAGCACACGTCTGAACTC532160.1151663968969131Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAAAAAGGGGTTGTAGGACACAGATCGGAAGAGCACACGTCTGAACTCCA523560.11330524421104148Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC502270.10869780924799412No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG500330.10827796782815796TruSeq Adapter, Index 8 (100% over 49bp)
TGCATGGTTGTCGTCAGCTCGTGAGATCGGAAGAGCACACGTCTGAACTC499060.10800312318733737Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAT477230.10327882514866553TruSeq Adapter, Index 8 (100% over 41bp)
CTTAAAACTCAAAGGACCTGGCGGAGATCGGAAGAGCACACGTCTGAACT474700.10273129999805444Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGGCTAGTTGATTCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC470380.10179639539305847Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGAGGCGATGAAGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC466030.10085499839497221Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)