FastQCFastQC Report
Tue 19 Sept 2023
SRR16913151_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16913151_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47250342
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACGAAGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT34811487.367455668363204TruSeq Adapter, Index 2 (100% over 37bp)
AGGACCGACTGACCCATGTTCAGATCGGAAGAGCACACGTCTGAACTCCA11836412.505042185726402Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTC9178431.9425108076466409TruSeq Adapter, Index 2 (100% over 50bp)
ACGGTCTGTATTCGTACTGAAAATCAGATCGGAAGAGCACACGTCTGAAC7298841.5447168615202826Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT6899511.4602031875240185TruSeq Adapter, Index 2 (100% over 43bp)
AGGACGAAGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT6672381.4121336941857479TruSeq Adapter, Index 2 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG5600291.1852379819811676TruSeq Adapter, Index 2 (100% over 49bp)
AGGGCTAGTTGATTCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC5129001.0854947885879853Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGGGCTGGGCCTCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC4168090.8821290648012663Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGGACCGACTGACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC2937910.6217753937103778Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCA2799660.5925163462308908No Hit
ACGGAATCGAGAAAGAGCTATCAGATCGGAAGAGCACACGTCTGAACTCC2693960.5701461377782197Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGACTTAATCAACGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2594540.5491050202345625Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGGC2178980.46115645046548026No Hit
CAGGACGAAGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA2175240.46036492180310573TruSeq Adapter, Index 2 (100% over 36bp)
AAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCA2015410.42653871161398155No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT1714550.36286509841558395No Hit
AGGACGAAGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT1688920.3574407990528407TruSeq Adapter, Index 2 (100% over 37bp)
AGAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATC1581000.3346007527310596No Hit
AGGACGAAGGGCACTCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGT1559170.32998068035147765Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAAAAAGGGGTTGTAGGACATCAGATCGGAAGAGCACACGTCTGAACTCC1556370.32938809204809566Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGACGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA1520430.3217817978968279TruSeq Adapter, Index 2 (100% over 40bp)
ATTCAGAGCACTGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1518160.32130137809372894Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGGGCCAGCGAGAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1422310.3010158106368838Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGACCTGTTATTGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1360290.2878899797169722Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCAGGACGAAGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG1302450.275648798478538TruSeq Adapter, Index 2 (100% over 35bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC1294260.2739154776911456No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAGATCGGAAGAGCACACGT1282770.2714837492604815No Hit
ATTCGTACTGAAAATCAAGATCAGATCGGAAGAGCACACGTCTGAACTCC1244460.2633758714381369Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACAGACCTGTTATTGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1198280.2536023972059292Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AATCGGTAGTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA1178960.24951353791259331TruSeq Adapter, Index 2 (100% over 36bp)
AAAGGGCAGGGACTTAATCAACGCAGATCGGAAGAGCACACGTCTGAACT1133810.23995805152055832Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGGACTTAATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT1097010.23216974810468036TruSeq Adapter, Index 2 (100% over 37bp)
CGGGAGGGAGGAAGACGAACAGATCGGAAGAGCACACGTCTGAACTCCAG1093280.23138033582910364Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGAGGGC1035910.21923862477016567No Hit
AGGACCGACTGACCCATGTTCAACTGCTGTTCAGATCGGAAGAGCACACG993190.21019742036999436No Hit
AGGACCGACTGACCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC940060.1989530573133206Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCT935420.19797105383914468Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCACA906010.19174676026683574No Hit
ATAGGAAGATAGGAAGTTGCTTTAGATCGGAAGAGCACACGTCTGAACTC886280.18757112911479032Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACGCTTGGTGAATTCTGCTTCACAAGATCGGAAGAGCACACGTCTGAAC874350.18504627966502338Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGGGGGCGGCCGCCTTTCCGGCCGCGCCCCGTTTCCCAGGACGAAGGGC859140.18182725534558036No Hit
AGGACCGACTGACCCATGTTTAGATCGGAAGAGCACACGTCTGAACTCCA769530.16286231325055806Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGGCTAGTTGATTCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC766350.1621893022488599Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACTTACTGGGAATTCCTCGTTCAGATCGGAAGAGCACACGTCTGAACTCC753410.15945069773251588Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGACCCA742230.1570845772925834No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT737760.15613855239396998No Hit
ACGGTCTGTATTCGTACTGAAAATCAAGATCGGAAGAGCACACGTCTGAA713870.15108250433404272Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGCTTGGGCTGCAGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC692280.1465132252376078Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGGCGGCTCGGGCCTGCTTTGAACAGATCGGAAGAGCACACGTCTGAACT684200.14480318470499112Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGCTTGGGCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA672270.14227833525522418TruSeq Adapter, Index 2 (100% over 36bp)
AAAGGAAAAGACTCATATCAACATTGAGATCGGAAGAGCACACGTCTGAA657740.13920322523803108Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAGATCGGAA655260.13867836131217845No Hit
ACTGGGCAGAAATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG653220.1382466184054287TruSeq Adapter, Index 2 (100% over 35bp)
AAGGGCCAGCGAGAGCTCACCGGACGCAGATCGGAAGAGCACACGTCTGA647340.13700218296832645Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGACGAAGGGCACTCCGCACCAGATCGGAAGAGCACACGTCTGAACTCC644340.13636726692898857Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACA633430.13405828893259653No Hit
CGGGAGGGAGGAAGACGAACGGAAGGACAGATCGGAAGAGCACACGTCTG615970.13036307758365007Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTCAGAGCACTGGGCAGAAATCAGATCGGAAGAGCACACGTCTGAACTC605990.12825092355945278Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGACCTCTAATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA601570.12731548059482828TruSeq Adapter, Index 2 (100% over 36bp)
AGTGGGAATCTCGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC592900.12548057324114184Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG588720.1245959235596644Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAGATCGGAAG587090.12425095251162414No Hit
TAAAAAGGGGTTGTAGGACATAGATCGGAAGAGCACACGTCTGAACTCCA583770.12354831209475689Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATCTCGGGTGGCTGAACGCCAGATCGGAAGAGCACACGTCTGAACTCCA569260.12047743485115937Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCTCTCAGGACCGACTGACCCATGTTCAGATCGGAAGAGCACACGTCTG559520.1184160741101091Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAACGGAAGGACGGACGGCGCCGGACGCGCAGATCGGAAGAGCACACGTC543490.11502350607324703No Hit
AGGGACTTAATCAACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA526210.11136638968666089Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACAGATCG493990.10454739142417213No Hit
ACGTGTTAGACTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA487420.10315692529802219TruSeq Adapter, Index 2 (100% over 36bp)
AGGACCGACTGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG484960.10263629414576513TruSeq Adapter, Index 2 (100% over 35bp)
ATCTCTCAGGACCGACTGACCCAGATCGGAAGAGCACACGTCTGAACTCC478350.101237362472424Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGTCGGACGACCGATTTGCACGTCAGATCGGAAGAGCACACGTCTGAAC475400.1006130283670751Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGTGTTAGACTCCTTGGTCAGATCGGAAGAGCACACGTCTGAACTCCAG474190.10035694556454215Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCCGCGCCCCGTTTCCCAGG473550.1002214968094834No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGTAGATCGGAAGAGCA472950.10009451360161585No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)