FastQCFastQC Report
Tue 19 Sept 2023
SRR16913152_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16913152_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41463042
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACGAAGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC9773162.357077418487529TruSeq Adapter, Index 4 (100% over 37bp)
TAAAAAGGGGTTGTAGGACATCAGATCGGAAGAGCACACGTCTGAACTCC6025721.4532749430203409Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCT5429511.3094818272137387TruSeq Adapter, Index 4 (100% over 43bp)
AGGACCGACTGACCCATGTTCAGATCGGAAGAGCACACGTCTGAACTCCA3888140.9377363098443188Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCT2832890.683232552015841Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC2433550.5869202746870333No Hit
ACGGTCTGTATTCGTACTGAAAATCAGATCGGAAGAGCACACGTCTGAAC2273660.5483582222452468Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGACGAAGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC2176350.5248891289741837TruSeq Adapter, Index 4 (100% over 37bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1952620.4709302322776993No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTC1814950.4377271691739357TruSeq Adapter, Index 4 (100% over 50bp)
ATAGGAAGATAGGAAGTTGCTTTAGATCGGAAGAGCACACGTCTGAACTC1724400.41588844349625864Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAAGGAAAAGACTCATATCAACATTGAGATCGGAAGAGCACACGTCTGAA1645520.39686427252491513Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGTGCTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA1420880.3426859032677824TruSeq Adapter, Index 4 (100% over 36bp)
AAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCA1308720.31563530722130806No Hit
ACGGGCTGGGCCTCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1294810.312280512365687Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACCGGAGTGGACGTACCGCTGGTGTTCCAGTTGAGATCGGAAGAGCACAC1250170.30151429796202606No Hit
TAAAAAGGGGTTGTAGGACATAGATCGGAAGAGCACACGTCTGAACTCCA1226380.29577665816222554Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT1133400.2733518683940267No Hit
AGGGCTAGTTGATTCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1074530.25915368197056066Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GAAGGACGTGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC1054200.25425052025849915TruSeq Adapter, Index 4 (100% over 37bp)
AAAGGGAGCAGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1049430.2531000981548821Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAAGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA1048950.25298433240860624TruSeq Adapter, Index 4 (100% over 40bp)
CTTAGTTGAACAGGGCCCTGAAGCGAGATCGGAAGAGCACACGTCTGAAC1019480.2458767979445406Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTAATTTTTTATCAACCTGTACCGAGAACGAGATCGGAAGAGCACACGTC1012790.2442633128558199No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT965680.23290138721611406Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATAATTTGTGGTAGTGGGGGACTGAGATCGGAAGAGCACACGTCTGAAC964310.23257097248195152Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTC942060.22720474778478625No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC891690.21505657978495643No Hit
AGAGGCGATGAAGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC864490.20849652082932071Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GAGAGAACTCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG862140.20792975102984484TruSeq Adapter, Index 4 (100% over 35bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAGATCGGAAGAGCACACGT837880.20207875727014918No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG821440.19811378046019876Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAGGAAGATAGGAAGTTGCTTTTAGATCGGAAGAGCACACGTCTGAACT799390.19279579149064846Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAAAAAGGGGTTGTAGGACATTAGATCGGAAGAGCACACGTCTGAACTCC794490.19161401616408175Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACGCTCGCTGTGTGCCTAATACATGAGATCGGAAGAGCACACGTCTGAA761160.18357553215704725Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAAGACTGACGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG747710.18033167947494058TruSeq Adapter, Index 4 (100% over 35bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCA740640.17862654650375145No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC706250.1703324131403576No Hit
ACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGGC638280.15393950111041058No Hit
CTTTGGTGACTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA623570.15039176334432963TruSeq Adapter, Index 4 (100% over 36bp)
ACGGAATCGAGAAAGAGCTATCAGATCGGAAGAGCACACGTCTGAACTCC619330.1493691659188923Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGGTCTGTATTCGTACTGAAAATCAAGATCGGAAGAGCACACGTCTGAA579930.1398667275787435Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATAGGAAGATAGGAAGTTGCTTAGATCGGAAGAGCACACGTCTGAACTCC573660.13835453751801424Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTAAAACTCAAAGGACCTGGCGGTGAGATCGGAAGAGCACACGTCTGAA560840.13526262737789474Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG556880.13430755997111837TruSeq Adapter, Index 4 (100% over 49bp)
AGGACCGACTGACCCATGTTTAGATCGGAAGAGCACACGTCTGAACTCCA550500.1327688402602009Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGACCGACTGACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT549430.1325107791174608Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAGGACGAAGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA545420.1315436527787807TruSeq Adapter, Index 4 (100% over 36bp)
ACACATTGATCATCGACACTTCGAACGAGATCGGAAGAGCACACGTCTGA501130.12086185089844591Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGACTTAATCAACGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA498080.12012625605231764Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGGACGAAGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC493390.11899512823974662TruSeq Adapter, Index 4 (100% over 37bp)
ACGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAT474680.11448267592136631TruSeq Adapter, Index 4 (100% over 41bp)
AACGCTTGGTGAATTCTGCTTCACAAGATCGGAAGAGCACACGTCTGAAC462470.11153788475047248Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCAGGATGAACGCTCGCTGTGTGAGATCGGAAGAGCACACGTCTGAACT459500.1108215841953902Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTCGTACTGAAAATCAAGATCAGATCGGAAGAGCACACGTCTGAACTCC456480.11009322470840419Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTAGTTGAACAGGGCCCTGAAGCGCGAGATCGGAAGAGCACACGTCTGA454350.10957951420930476Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCGTGCTGTAAGACCTAAAGTTCTTATGAGATCGGAAGAGCACACGTCT447310.10788161659725785Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATGGTTGTCGTCAGCTCGTGTCGTGAGATGAGATCGGAAGAGCACACGT425820.1026986876650295No Hit
CGACTGTTTAATTAAAACAGATCGGAAGAGCACACGTCTGAACTCCAGTC419980.10129020441867241Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG417420.1006727871052008Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)