FastQCFastQC Report
Tue 19 Sept 2023
SRR16913153_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16913153_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39484812
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAAAAGGGGTTGTAGGACATCAGATCGGAAGAGCACACGTCTGAACTCC5452671.380953770275011Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGACGAAGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA3064220.7760502949843093TruSeq Adapter, Index 7 (100% over 37bp)
TAAAAAGGGGTTGTAGGACATAGATCGGAAGAGCACACGTCTGAACTCCA2788160.7061348044407556Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCT2483800.629052001058027Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT2462200.6235815432019784TruSeq Adapter, Index 7 (100% over 43bp)
ACCGGAGTGGACGTACCGCTGGTGTTCCAGTTGAGATCGGAAGAGCACAC2444320.6190532197544717No Hit
ATAGGAAGATAGGAAGTTGCTTTAGATCGGAAGAGCACACGTCTGAACTC1780840.4510189892761804Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT1531420.3878503967550865No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC1414470.35823141313171253No Hit
ACGGTGCTGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG1381940.3499928022957283TruSeq Adapter, Index 7 (100% over 36bp)
AGGACCGACTGACCCATGTTCAGATCGGAAGAGCACACGTCTGAACTCCA1379110.3492760710118108Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAGGAAAAGACTCATATCAACATTGAGATCGGAAGAGCACACGTCTGAA1374630.3481414575305563Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1193160.3021820136816151No Hit
GAAGGACGTGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA1032090.2613891133633864TruSeq Adapter, Index 7 (100% over 37bp)
AAAGGGAGCAGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1013540.25669110441756693Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGACGAAGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA1000680.25343415589771584TruSeq Adapter, Index 7 (100% over 37bp)
GAGAGAACTCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA985940.24970107493483823TruSeq Adapter, Index 7 (100% over 35bp)
CATGGTTGTCGTCAGCTCGTGTCGTGAGATGAGATCGGAAGAGCACACGT980820.24840437381340452No Hit
TAAAAAGGGGTTGTAGGACATTAGATCGGAAGAGCACACGTCTGAACTCC925610.2344217822285693Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAAGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA883920.22386329204252003TruSeq Adapter, Index 7 (100% over 40bp)
CTTAAAACTCAAAGGACCTGGCGGTGAGATCGGAAGAGCACACGTCTGAA870430.2204467885018675Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTAGTTGAACAGGGCCCTGAAGCGAGATCGGAAGAGCACACGTCTGAAC866670.21949452361581462Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACACATTGATCATCGACACTTCGAACGAGATCGGAAGAGCACACGTCTGA797270.20191814513388084Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT780870.19776464935428842Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAGACTGACGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA763510.1933680221144272TruSeq Adapter, Index 7 (100% over 35bp)
TTAATTTTTTATCAACCTGTACCGAGAACGAGATCGGAAGAGCACACGTC696710.1764501246707215No Hit
CATAATTTGTGGTAGTGGGGGACTGAGATCGGAAGAGCACACGTCTGAAC666460.16878895105287572Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGGTCTGTATTCGTACTGAAAATCAGATCGGAAGAGCACACGTCTGAAC651990.16512425081319876Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATAGGAAGATAGGAAGTTGCTTTTAGATCGGAAGAGCACACGTCTGAACT644650.16326530818989338Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC632910.16029201304035587No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG598080.15147089974747757Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGCTCGCTGTGTGCCTAATACATGAGATCGGAAGAGCACACGTCTGAA585470.14827726671207145Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATAGGAAGATAGGAAGTTGCTTAGATCGGAAGAGCACACGTCTGAACTCC579940.14687672819614792Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTAAAACTCAAAGGACCTGGCGAGATCGGAAGAGCACACGTCTGAACTC571320.1446936102924841Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCACAAGAACTGCTAACTCATGAGATCGGAAGAGCACACGTCTGAACTC544860.13799229941882463Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGTGATCGTATAGTGGTTAGTACTCTAGATCGGAAGAGCACACGTCTG537140.13603711725916284Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTC531640.1346441766013727TruSeq Adapter, Index 7 (100% over 50bp)
AGAGGCGATGAAGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC531470.13460112207195007Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGGAAACTCGACTGCATAATTTGAGATCGGAAGAGCACACGTCTGAACT528810.13392744531745523Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAAAACTAAGTGGAGGACCGAACCGTAGTACGAGATCGGAAGAGCACACG495580.125511551125025No Hit
CATGGGCCAGAAGGACTCCTACGTGAGATCGGAAGAGCACACGTCTGAAC470880.11925598126185835Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTAAAACTCAAAGGACCTGGCGGAGATCGGAAGAGCACACGTCTGAACT458030.11600156536138502Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG455290.11530762765186776TruSeq Adapter, Index 7 (100% over 49bp)
CTCCAATTTCCTGTAGGACGAGTGCACCGAGATCGGAAGAGCACACGTCT453800.11493026736457554Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGGCTGGGCCTCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC450180.11401345914981184Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTCGTGCTGTAAGACCTAAAGTTCTTATGAGATCGGAAGAGCACACGTCT449550.11385390412901042Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC438180.11097431589645153No Hit
CATAATCTTGAGTTGTGATGCATAGTGAGATCGGAAGAGCACACGTCTGA435410.11027278032880086Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACCAATGTTAAGGAACTCTGCAAAATAACCCCGAGATCGGAAGAGCACA427520.10827454363971645No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC418920.10609649097480824No Hit
TGCATGGTTGTCGTCAGCTCGTGAGATCGGAAGAGCACACGTCTGAACTC411260.10415650453141324Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGGTGCTGAAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG400480.10142634084214455TruSeq Adapter, Index 7 (100% over 36bp)
CTCAGGATGAACGCTCGCTGTGTGAGATCGGAAGAGCACACGTCTGAACT399650.10121613343378716Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTC399460.10116801366560894No Hit
ACGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAT397360.10063616359627088TruSeq Adapter, Index 7 (100% over 41bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGAGATCGGAAGAGCAC395020.10004353066186562No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)