FastQCFastQC Report
Tue 19 Sept 2023
SRR16913163_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16913163_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51693186
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGGTGCCGTC5194361.0048442361436185TruSeq Adapter, Index 1 (97% over 41bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTGGGGTGCCGTC4798900.9283428574125804TruSeq Adapter, Index 1 (97% over 41bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTGGGTTGCCGTC3331060.6443905392095585TruSeq Adapter, Index 1 (97% over 41bp)
AGGGGCTCTCGCTTCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG2958050.5722320926398308Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGTTGCCGTC2954800.5716033830841845TruSeq Adapter, Index 1 (97% over 41bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTGTGCCGTC2804190.542468015030066TruSeq Adapter, Index 1 (98% over 50bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT2475320.4788484114714849No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGATGCCGTC2424140.4689476868382615TruSeq Adapter, Index 1 (98% over 50bp)
AATACACGAAGACTCTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC2133950.41281069423734107Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTC2131000.41224001940990834TruSeq Adapter, Index 1 (100% over 50bp)
CTCTCGCTTCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA2059030.3983174881114892Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTGGGATGCCGTC1841570.3562500481204621TruSeq Adapter, Index 1 (97% over 41bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC1798640.34794527851311No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCT1794320.3471095784268356Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGACTCTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG1706140.330051237313947TruSeq Adapter, Index 1 (100% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTGGGGTGGCGTC1660110.3211467755150553TruSeq Adapter, Index 1 (97% over 41bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTTTGCCGTC1561650.302099777715384TruSeq Adapter, Index 1 (98% over 50bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTGGGTTGGCGTC1487060.287670409790567TruSeq Adapter, Index 1 (97% over 41bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT1289610.24947388617138053Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTGGTGTGCCGTC1106120.21397791190506232TruSeq Adapter, Index 1 (97% over 42bp)
TCTGAAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA1009000.1951901358914113TruSeq Adapter, Index 1 (100% over 40bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTGGTATGCCGTC865920.1675114395154518TruSeq Adapter, Index 1 (98% over 50bp)
TCCGAACTGATATAACCTACCAGATCGGAAGAGCACACGTCTGAACTCCA863370.16701814432563702Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTC861760.16670669128422458No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGTTT850050.16444140239295757TruSeq Adapter, Index 1 (97% over 45bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGGTGGCGTC843670.1632071971729504TruSeq Adapter, Index 1 (97% over 41bp)
CCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA833920.1613210685060116TruSeq Adapter, Index 1 (100% over 37bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTGGTTTGCCGTC776640.15024030439911365TruSeq Adapter, Index 1 (97% over 42bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTGGGGTGCCGGC753490.14576195787197177TruSeq Adapter, Index 1 (97% over 41bp)
AGCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG718850.13906088125425273TruSeq Adapter, Index 1 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGTTG708390.13703740373054196TruSeq Adapter, Index 1 (97% over 45bp)
ATAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT663470.1283476704260403TruSeq Adapter, Index 1 (100% over 43bp)
TAAAAAGGGGTTGTAGGACATCAGATCGGAAGAGCACACGTCTGAACTCC606170.11726303733726143Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTGGGTTT565850.10946316986536679TruSeq Adapter, Index 1 (97% over 45bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT546230.10566769864020376No Hit
CCGTGGCTCCGGATCTCTTCGTCTTTGCAGAGATCGGAAGAGCACACGTC540710.10459985964107532No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)