FastQCFastQC Report
Tue 19 Sept 2023
SRR16913164_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16913164_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34169430
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGGTGGGGGG17507415.123705604688167TruSeq Adapter, Index 4 (97% over 41bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGGTGGCGGG6065101.7750076603560552TruSeq Adapter, Index 4 (97% over 41bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTGGGGTGGGGGG4778151.3983698294059925TruSeq Adapter, Index 4 (97% over 41bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGGTGGGGGC4500971.317250536517583TruSeq Adapter, Index 4 (97% over 41bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGGTGGCGGC2589890.7579552834214677TruSeq Adapter, Index 4 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGGTG1686830.4936664146870463TruSeq Adapter, Index 4 (97% over 45bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT1330270.3893158299684835No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC1171270.34278300808646794No Hit
AGGGGCTCTCGCTTCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG1158020.3389052729296333Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGTTGGGGGG1074770.314541389774427TruSeq Adapter, Index 4 (97% over 41bp)
AATACACGAAGACTCTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1055080.3087789290017422Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAGACTCTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA990660.2899258196580979TruSeq Adapter, Index 4 (100% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTGGGGTGGCGGG849370.24857599321967033TruSeq Adapter, Index 4 (97% over 41bp)
TGCATGGTTGTCGTCAGCTCGTGAGATCGGAAGAGCACACGTCTGAACTC844500.24715074263749792Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTGGGGTGGGGGC739980.21656199708335785TruSeq Adapter, Index 4 (97% over 41bp)
AGCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA735410.2152245442783213TruSeq Adapter, Index 4 (100% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGGGGGGGGG713820.20890603091710927TruSeq Adapter, Index 4 (97% over 41bp)
CTCTCGCTTCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT704700.20623697849217854Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT591140.17300259325367734Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGGTTGGGGG560840.1641350177629536TruSeq Adapter, Index 4 (97% over 41bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGGTGGGGTG545050.15951392809303522TruSeq Adapter, Index 4 (97% over 41bp)
GCCGTGATCGTATAGTGGTTAGTACTAGATCGGAAGAGCACACGTCTGAA536480.15700583825952028Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCCGGTCTTATCTATGAGATCGGAAGAGCACACGTCTGAACTCCAGTC487130.14256310392066826Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCT485680.14213874799784484Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC468420.13708744921996063TruSeq Adapter, Index 4 (100% over 37bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTGGGTTGGGGGG451830.13223223214434657TruSeq Adapter, Index 4 (97% over 41bp)
ATAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCT438930.1284569277275038TruSeq Adapter, Index 4 (100% over 43bp)
TCCGAACTGATATAACCTACCAGATCGGAAGAGCACACGTCTGAACTCCA428230.12532547367632413Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTTCCGGCTCGAATTGCTCTCGCTCACGCTTGAGATCGGAAGAGCACACG424850.1243362853872599No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGTTGGCGGG416340.12184575510917216TruSeq Adapter, Index 4 (97% over 41bp)
ACCGATCTTCGCTTCCAGAGCTCTGAGATCGGAAGAGCACACGTCTGAAC397050.11620035803933516Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGAGCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA391500.11457609916232141TruSeq Adapter, Index 4 (100% over 36bp)
ACTCATAAGGTTACTGTGCTCTTTGAGATCGGAAGAGCACACGTCTGAAC382220.11186022125625157Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTGAAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA355260.10397012768430729TruSeq Adapter, Index 4 (100% over 40bp)
CCGTGGCTCCGGATCTCTTCGTCTTTGCAGAGATCGGAAGAGCACACGTC352720.10322677317122352No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)