FastQCFastQC Report
Tue 19 Sept 2023
SRR16913165_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16913165_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34555178
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTC3891221.1260888310284496No Hit
TGCATGGTTGTCGTCAGCTCGGGAGATCGGAAGAGCACACGTCTGAACTC3053940.8837865051657382Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCTCGCTTCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1855090.5368486309056201Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGCATGGTTGTCGTCAGCTCGTGAGATCGGAAGAGCACACGTCTGAACTC1795100.5194879910617158Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA1328260.38438812267151395TruSeq Adapter, Index 1 (100% over 37bp)
CTCTCGCTTCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC1203900.34839930501877314TruSeq Adapter, Index 1 (100% over 36bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCT1109190.32099096696882884Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCTGGCGCCAAGCGCCCGGCCAGATCGGAAGAGCACACGTCTGAACTC1049940.30384447737470777Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACGTCTGA952360.2756055836262803Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTCTGGCGCCAAGCGCCCGGCCGCGAGATCGGAAGAGCACACGTCTGAA717890.20775178759027085Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAAAAAGGGGTTGTAGGACATCAGATCGGAAGAGCACACGTCTGAACTCC651980.1886779457481018Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACTACTCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG639040.18493321029919163TruSeq Adapter, Index 1 (100% over 39bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT613610.17757396590461783Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAAC576650.16687802910463956Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGTTGTCGTCAGCTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT528590.15296983855791454Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG480730.13911952645707684Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTACTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAT475920.13772754983348662TruSeq Adapter, Index 1 (100% over 41bp)
CGGGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCT463650.13417670717829902TruSeq Adapter, Index 1 (100% over 43bp)
TAAAAAGGGGTTGTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC461190.13346480229388485Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCTGAACT444390.1286030128393493Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCGAGATCGGAAGAGCACACG438190.1268087810168421No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTGGGTTTGGGGG434260.12567146955515612TruSeq Adapter, Index 1 (97% over 41bp)
GCCGTGATCGTATAGTGGTTAGTACTCGGAGATCGGAAGAGCACACGTCT433100.1253357745690096Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT429690.12434894706663065No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTAGATCGGAAGAGCACACGTCTG426580.1234489372330827Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCTGGGCTGTAGTGCGCTATGAGATCGGAAGAGCACACGTCTGAACTCC403550.11678423418915683Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CATAATTTGTGGTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA389280.11265460707509595Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTGGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATC384960.11140443264392966TruSeq Adapter, Index 1 (100% over 42bp)
CGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTG360820.10441850422532911TruSeq Adapter, Index 1 (97% over 43bp)
CGGTCGAACTTGACTATCTAGAGATCGGAAGAGCACACGTCTGAACTCCA356220.10308729996992058Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTTCTGGCGCCAAGCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC356010.10302652760173887Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTGGGTTTGCGGG353330.10225095642684867TruSeq Adapter, Index 1 (97% over 41bp)
TAAAAAGGGGTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT351860.10182554984957681TruSeq Adapter, Index 1 (100% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)