FastQCFastQC Report
Tue 19 Sept 2023
SRR16913166_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16913166_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74629852
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTC822469711.02065296873428TruSeq Adapter, Index 2 (100% over 50bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTGGTATGCCGTC748184910.025276480516135TruSeq Adapter, Index 2 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG8912721.1942566896689009TruSeq Adapter, Index 2 (100% over 49bp)
AGCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT2857740.38292183669344537TruSeq Adapter, Index 2 (100% over 39bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT2635650.35316296754816023No Hit
CTCTCGCTTCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC2558700.3428520801568788Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC2352830.31526660403935947No Hit
AAGACTCTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT2335420.3129337573924172TruSeq Adapter, Index 2 (100% over 39bp)
AGGGGCTCTCGCTTCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG2316170.31035436061162225Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGTGGCTCCGGATCTCTTCGTCTTTGCAGAGATCGGAAGAGCACACGTC2248830.3013311616911688No Hit
AATACACGAAGACTCTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1864100.2497794046275209Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGCATGGTTGTCGTCAGCTCGTGAGATCGGAAGAGCACACGTCTGAACTC1773590.23765154994545615Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCT1766760.2367363665681663Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT1453240.19472636767389007Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGAGCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA1427060.191218388052009TruSeq Adapter, Index 2 (100% over 36bp)
AGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTA1313700.17602875589248118TruSeq Adapter, Index 2 (100% over 47bp)
CTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTC1150260.15412867226374777No Hit
CCGTGGCTCCGGATCTCTTCGTCTTTGAGATCGGAAGAGCACACGTCTGA1124520.15067965028257058Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTGGTATG1121340.15025354733384705TruSeq Adapter, Index 2 (97% over 49bp)
CGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT970680.1300659151782855TruSeq Adapter, Index 2 (100% over 41bp)
ATAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT967630.1296572315324972TruSeq Adapter, Index 2 (100% over 43bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTAT942800.1263301446718667TruSeq Adapter, Index 2 (100% over 48bp)
CCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT904300.1211713511102769TruSeq Adapter, Index 2 (100% over 37bp)
GCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA840390.11260775379803782TruSeq Adapter, Index 2 (100% over 40bp)
CTTCCGGCTCGAATTGCTCTCGCTCACGCTTGAGATCGGAAGAGCACACG791660.10607819509008273No Hit
TGGTTGTCGTCAGCTCGTGAGATCGGAAGAGCACACGTCTGAACTCCAGT789220.1057512481734521Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCTGAAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA765030.10250991788111814TruSeq Adapter, Index 2 (100% over 40bp)
TAAAAAGGGGTTGTAGGACATCAGATCGGAAGAGCACACGTCTGAACTCC749250.10039548249405612Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)