FastQCFastQC Report
Tue 19 Sept 2023
SRR16913167_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16913167_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12639291
Total Bases631.9 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTGGTATGCCGTC4058623.2111136613596445TruSeq Adapter, Index 5 (98% over 50bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTGGTTTGCCGTC2494351.9734888610444998TruSeq Adapter, Index 5 (97% over 42bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTC2328411.842199851241656TruSeq Adapter, Index 5 (100% over 50bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTGGTATGGCGTC1469021.162264560567519TruSeq Adapter, Index 5 (97% over 45bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTGGTTTGGCGTC1300881.0292349468020001TruSeq Adapter, Index 5 (97% over 42bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTTTGCCGTC963900.7626218907373838TruSeq Adapter, Index 5 (98% over 50bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGGCGTC799850.6328282179752013TruSeq Adapter, Index 5 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTGGTTTG646270.511318237708112TruSeq Adapter, Index 5 (97% over 46bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC556340.4401670948156823No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTGGTATGCCGTG549120.43445474908363135TruSeq Adapter, Index 5 (97% over 49bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT545480.43157484070902397No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTGGTATG482700.38190433308324023TruSeq Adapter, Index 5 (97% over 49bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTGGTTTGCCGTG469420.37139741461763953TruSeq Adapter, Index 5 (97% over 42bp)
AGCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTG457350.3618478283315101TruSeq Adapter, Index 5 (100% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTTTGGCGTC456200.3609379671692028TruSeq Adapter, Index 5 (97% over 45bp)
AATACACGAAGACTCTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC439870.3480179386644393Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTGGTTTGGCGTG384460.3041784543136162TruSeq Adapter, Index 5 (97% over 42bp)
AAGACTCTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTG372720.2948899586218879TruSeq Adapter, Index 5 (100% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTGGTATGGCGTG331860.2625621959333003TruSeq Adapter, Index 5 (97% over 45bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTGGTATGCCGGC331720.2624514302265847TruSeq Adapter, Index 5 (97% over 48bp)
TGCATGGTTGTCGTCAGCTCGTGAGATCGGAAGAGCACACGTCTGAACTC316000.25001402372965387Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTG293350.23209371475029728TruSeq Adapter, Index 5 (97% over 49bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT281390.22263115866230151Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCT270610.214102199245195Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTGGTTTGCCGGC268710.21259895036833948TruSeq Adapter, Index 5 (97% over 42bp)
GCCGTGATCGTATAGTGGTTAGTACTAGATCGGAAGAGCACACGTCTGAA257490.20372187015869797Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGTGGCTCCGGATCTCTTCGTCTTTGCAGAGATCGGAAGAGCACACGTC253820.20081822627550866No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGGC250780.19841302807253985TruSeq Adapter, Index 5 (98% over 50bp)
CTCTCGCTTCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA231340.18303241851144972Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTGGGATGCCGTC207360.16405983531829438TruSeq Adapter, Index 5 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG204390.16171001996868337TruSeq Adapter, Index 5 (100% over 49bp)
AGGGGCTCTCGCTTCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG202860.160499509031005Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAAAAAGGGGTTGTAGGACATAGATCGGAAGAGCACACGTCTGAACTCCA196730.15564955344409745Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTGGTTTGGCGGC192740.15249273080270087TruSeq Adapter, Index 5 (97% over 42bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTGGTGTGCCGTC189090.14960491059189948TruSeq Adapter, Index 5 (97% over 42bp)
CCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG182740.14458089460872448TruSeq Adapter, Index 5 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTTTG182340.14426442116096544TruSeq Adapter, Index 5 (97% over 49bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTGGTATGGCGGC182030.14401915423895217TruSeq Adapter, Index 5 (97% over 45bp)
ATAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT181840.14386882935126663TruSeq Adapter, Index 5 (100% over 43bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGGCGTG171690.1358383156143806TruSeq Adapter, Index 5 (97% over 49bp)
GGGAGCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA169420.13404232879834793TruSeq Adapter, Index 5 (100% over 36bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTTTGCCGTG169330.13397112227260216TruSeq Adapter, Index 5 (97% over 49bp)
CTTCCGGCTCGAATTGCTCTCGCTCACGCTTGAGATCGGAAGAGCACACG167790.13275269949872978No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTGGGTTGCCGTC158130.1251098657353486TruSeq Adapter, Index 5 (97% over 41bp)
TTTCCGGTCTTATCTATGAGATCGGAAGAGCACACGTCTGAACTCCAGTC147520.11671540753353966Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGTGGCTCCGGATCTCTTCGTCTTTGAGATCGGAAGAGCACACGTCTGA143720.11370890977982864Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGGATGCCGTC143200.11329749429774186TruSeq Adapter, Index 5 (98% over 50bp)
GCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGA140400.11108218016342847TruSeq Adapter, Index 5 (100% over 40bp)
TGGTTGTCGTCAGCTCGTGAGATCGGAAGAGCACACGTCTGAACTCCAGT139960.11073405937089352Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAT136620.1080915060821054TruSeq Adapter, Index 5 (100% over 41bp)
TCCGAACTGATATAACCTACCAGATCGGAAGAGCACACGTCTGAACTCCA136140.10771173794479454Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTGTGCCGTC132860.10511665567317027TruSeq Adapter, Index 5 (98% over 50bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTGGGTTGGCGTC132460.10480018222541122TruSeq Adapter, Index 5 (97% over 41bp)
TCTGAAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGA130120.10294881255602074TruSeq Adapter, Index 5 (100% over 40bp)
TTCTGGGCTGTAGTGCGCTATGAGATCGGAAGAGCACACGTCTGAACTCC130110.10294090071982676Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTTTGCCGGC129210.1022288354623689TruSeq Adapter, Index 5 (97% over 48bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTGGGATGGCGTC128640.10177786079931224TruSeq Adapter, Index 5 (97% over 41bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGGCGGC127920.10120820859334595TruSeq Adapter, Index 5 (97% over 48bp)
ACTCATAAGGTTACTGTGCTCTTTGAGATCGGAAGAGCACACGTCTGAAC127330.10074141025790133Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGTGCTCTTCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA126990.10047240782730614TruSeq Adapter, Index 5 (100% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)