FastQCFastQC Report
Tue 19 Sept 2023
SRR16913168_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16913168_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33109937
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCATGGTTGTCGTCAGCTCGTGAGATCGGAAGAGCACACGTCTGAACTC2667450.8056342722730037Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTC2540330.7672409645478939No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTC1881240.5681798790496038TruSeq Adapter, Index 2 (100% over 50bp)
CCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT1286400.3885238440653028TruSeq Adapter, Index 2 (100% over 37bp)
TTCTGGGCTGTAGTGCGCTATGAGATCGGAAGAGCACACGTCTGAACTCC1216880.36752712637296775Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCTCGCTTCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA1108250.3347182448580316TruSeq Adapter, Index 2 (100% over 36bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCT1092410.3299341825990185Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCTGGCGCCAAGCGCCCGGCCAGATCGGAAGAGCACACGTCTGAACTC1082810.32703475092688944Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACTACTCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT897920.27119350906647754TruSeq Adapter, Index 2 (100% over 39bp)
CCTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAAC844720.25512582521676197Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTGGTATGCCGTC840620.25388752627345684TruSeq Adapter, Index 2 (98% over 50bp)
CTCTCGCTTCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC787890.23796179376602256Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACGTCTGA671040.20267027388182587Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CATAATTTGTGGTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC517180.15620084085330638Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTTCTGGCGCCAAGCGCCCGGCCGCGAGATCGGAAGAGCACACGTCTGAA511080.15435849364497434Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTACTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT485280.1465662710261273TruSeq Adapter, Index 2 (100% over 41bp)
GATTATGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG472490.14270338237128027TruSeq Adapter, Index 2 (100% over 38bp)
CATAATTTGTGGTAGTGGGGGACTGAGATCGGAAGAGCACACGTCTGAAC455680.13762635670372916Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGTTGTCGTCAGCTCGTGAGATCGGAAGAGCACACGTCTGAACTCCAGT453790.13705553109327875Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCTGAACT430810.13011501652811963Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT413550.1249020800009375No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC413090.1247631488999813No Hit
CTCGACTGCATAATTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA407320.12302046965537868Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCT403510.12186975771050244TruSeq Adapter, Index 2 (100% over 43bp)
CATGGTTGTCGTCAGCTCGTGAGATCGGAAGAGCACACGTCTGAACTCCA397010.119906600849165Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTC396960.11989149964253933TruSeq Adapter, Index 2 (100% over 44bp)
AAAAGATTGATCGCCGTTCTGGTAAAAAGAGATCGGAAGAGCACACGTCT394430.11912737858728031Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGTCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT391670.11829379198154319TruSeq Adapter, Index 2 (100% over 41bp)
TAAAAAGGGGTTGTAGGACATCAGATCGGAAGAGCACACGTCTGAACTCC388040.11719744438051936Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGTAGAACCTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG387570.11705549303823803TruSeq Adapter, Index 2 (100% over 35bp)
CTTCTGGCGCCAAGCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC359720.10864412094773843Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGTGGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATC357330.10792228327103129TruSeq Adapter, Index 2 (100% over 42bp)
CGGTCGAACTTGACTATCTAGAGATCGGAAGAGCACACGTCTGAACTCCA356070.10754173286406435Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGTTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATC352530.10647256743496673TruSeq Adapter, Index 2 (100% over 42bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)