FastQCFastQC Report
Tue 19 Sept 2023
SRR16913169_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16913169_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40867169
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTC39633559.698139354844962TruSeq Adapter, Index 3 (100% over 50bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGTATGCCGTC36896679.028437962022767TruSeq Adapter, Index 3 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG3964300.9700451724463713TruSeq Adapter, Index 3 (100% over 49bp)
AAGACTCTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGC2129260.5210196967644126TruSeq Adapter, Index 3 (100% over 39bp)
AGCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGC1996900.48863184039002067TruSeq Adapter, Index 3 (100% over 39bp)
AGGGGCTCTCGCTTCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG1736580.42493278651134364Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AATACACGAAGACTCTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1720630.42102989810720676Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCTCGCTTCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT1502670.36769613280528435Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC1340130.32792337536275146No Hit
GGGAGCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA1295570.3170197573509435TruSeq Adapter, Index 3 (100% over 36bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT1201120.29390829592331197No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT1042490.2550922967039875Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCT1018390.24919514243817573Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCATGGTTGTCGTCAGCTCGTGAGATCGGAAGAGCACACGTCTGAACTC863840.2113774996256775Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGTATG699780.17123280548256228TruSeq Adapter, Index 3 (97% over 49bp)
CCGTGGCTCCGGATCTCTTCGTCTTTGCAGAGATCGGAAGAGCACACGTC692000.16932907684405543No Hit
CGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCAT674250.16498573708396586TruSeq Adapter, Index 3 (100% over 41bp)
GCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA591850.14482285278924018TruSeq Adapter, Index 3 (100% over 40bp)
CTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTC553240.1353751712040538No Hit
ACGAATTTTTGAGCGGGTAAAGGTCGCCCTCAAGAGATCGGAAGAGCACA526850.12891766493539103No Hit
CCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG517260.12657103798895392TruSeq Adapter, Index 3 (100% over 37bp)
TCTGAAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA490470.12001565364119056TruSeq Adapter, Index 3 (100% over 40bp)
ATAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCT480100.11747816444050724TruSeq Adapter, Index 3 (100% over 43bp)
TAAAAAGGGGTTGTAGGACATCAGATCGGAAGAGCACACGTCTGAACTCC438030.10718383747110058Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGAACTGATATAACCTACCAGATCGGAAGAGCACACGTCTGAACTCCA436850.10689509713775378Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)