FastQCFastQC Report
Tue 19 Sept 2023
SRR16913170_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16913170_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46411416
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTC597675412.877766970092013TruSeq Adapter, Index 6 (100% over 50bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTGGTATGCCGTC11451982.467492049800851TruSeq Adapter, Index 6 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG4949971.0665414733306133TruSeq Adapter, Index 6 (100% over 49bp)
AGCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAT1762280.379708302802052TruSeq Adapter, Index 6 (100% over 39bp)
AAGACTCTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAT1689070.36393416654212835TruSeq Adapter, Index 6 (100% over 39bp)
AATACACGAAGACTCTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1666990.3591767163492706Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGCATGGTTGTCGTCAGCTCGTGAGATCGGAAGAGCACACGTCTGAACTC1467180.3161248086031247Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGGGCTCTCGCTTCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAG1353460.29162221639606944Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC1283830.2766194420786472No Hit
CTCTCGCTTCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1230810.2651955286173557Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT1115230.2402921729429673Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT1058450.22805811397781958No Hit
TAAAAAGGGGTTGTAGGACATAGATCGGAAGAGCACACGTCTGAACTCCA995170.21442353751930346Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGAGCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC899270.1937605178863752TruSeq Adapter, Index 6 (100% over 36bp)
GCCGTGATCGTATAGTGGTTAGTACTAGATCGGAAGAGCACACGTCTGAA699240.15066120800968452Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGGCGTC671960.14478334382213204TruSeq Adapter, Index 6 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTGGTATG661580.14254682511733752TruSeq Adapter, Index 6 (97% over 49bp)
ACGAATTTTTGAGCGGGTAAAGGTCGCCCTCAAGAGATCGGAAGAGCACA647220.13945275877814198No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCT620480.13369124527465398Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAAAAAGGGGTTGTAGGACATCAGATCGGAAGAGCACACGTCTGAACTCC608520.13111429308685604Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAT595630.12833695916539153TruSeq Adapter, Index 6 (100% over 41bp)
ATAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT559770.12061041188659273TruSeq Adapter, Index 6 (100% over 43bp)
CTTCCGGCTCGAATTGCTCTCGCTCACGCTTGAGATCGGAAGAGCACACG549160.11832433640895593No Hit
TTTCCGGTCTTATCTATGAGATCGGAAGAGCACACGTCTGAACTCCAGTC540320.11641963261797485Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA530880.11438565028914437TruSeq Adapter, Index 6 (100% over 37bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTAT522580.11259729718222775TruSeq Adapter, Index 6 (100% over 48bp)
CCGTGGCTCCGGATCTCTTCGTCTTTGCAGAGATCGGAAGAGCACACGTC501930.1081479608379111No Hit
GCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA498120.10732704212256743TruSeq Adapter, Index 6 (100% over 40bp)
ACCGATCTTCGCTTCCAGAGCTCTGAGATCGGAAGAGCACACGTCTGAAC498060.1073141142687825Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTAGAACCTTACCCACTCTTGACATCTTCTGAGATCGGAAGAGCACACG491700.10594376176757891No Hit
TCCGAACTGATATAACCTACCAGATCGGAAGAGCACACGTCTGAACTCCA480260.10347885097925044Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)