FastQCFastQC Report
Tue 19 Sept 2023
SRR16913171_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16913171_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35184007
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCATGGTTGTCGTCAGCTCGTGAGATCGGAAGAGCACACGTCTGAACTC4297051.221307737916264Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCT3253430.9246900161201083Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTC2994310.8510429184487144No Hit
CTCTCGCTTCTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT1281470.36421945914233134Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTC1211250.34426152768784973TruSeq Adapter, Index 3 (100% over 50bp)
CCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG1167090.3317103705669454TruSeq Adapter, Index 3 (100% over 37bp)
TAAAAAGGGGTTGTAGGACATCAGATCGGAAGAGCACACGTCTGAACTCC1016860.2890119934321295Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCTCGCTTCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA927360.26357429953899225TruSeq Adapter, Index 3 (100% over 36bp)
CTCTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACGTCTGA886100.2518473805442342Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGTATGCCGTC862970.24527337093810833TruSeq Adapter, Index 3 (98% over 50bp)
CACTACTCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGC862410.24511420771374903TruSeq Adapter, Index 3 (100% over 39bp)
TGGTTGTCGTCAGCTCGTGAGATCGGAAGAGCACACGTCTGAACTCCAGT730930.2077449563945346Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCGTGATCGTATAGTGGTTAGTACTCTAGATCGGAAGAGCACACGTCTG727880.2068780852618634Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT696030.19782567687642855Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCTGGCGCCAAGCGCCCGGCCAGATCGGAAGAGCACACGTCTGAACTC677730.19262445008040158Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAAC665810.18923654716189658Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCTGGGCTGTAGTGCGCTATGAGATCGGAAGAGCACACGTCTGAACTCC644850.183279295050163Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC641270.18226178729443748No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT634900.18045130561735054No Hit
TTGTCGTCAGCTCGTGTCGTGAGATGAGATCGGAAGAGCACACGTCTGAA605070.17197302171978307Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT601650.1710009891710174No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC539250.15326565845669596No Hit
TTCGTGCTGTAAGACCTAAAGTTCTTATGAGATCGGAAGAGCACACGTCT525070.14923541823988382Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTTGTCGTCAGCTCGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC501980.14267277743549792Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CACTACTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCAT488080.13872211883086538TruSeq Adapter, Index 3 (100% over 41bp)
CTTCTGGCGCCAAGCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC478980.13613571643502687Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCCGATTTAGCTCAGCGGTAGAGCAGCTGAGATCGGAAGAGCACACGTCT457960.13016141112068333Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTAGAACCTTACCCACTCTTGACATCTTCTGAGATCGGAAGAGCACACG440840.12529556397598487No Hit
GTGCATGGTTGTCGTCAGCTCGTGAGATCGGAAGAGCACACGTCTGAACT427380.12146996219049183Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAAGATTGATCGCCGTTCTGGTAAAAAGAGATCGGAAGAGCACACGTCT426520.12122553295308293Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC417610.11869313236550914No Hit
CGGTCGAACTTGACTATCTAGAGATCGGAAGAGCACACGTCTGAACTCCA413210.11744256417411468Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGTAGAACCTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT410390.11664106365144823TruSeq Adapter, Index 3 (100% over 35bp)
CATAATTTGTGGTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT407150.1157201907105123Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATTATGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG407060.11569461090659743TruSeq Adapter, Index 3 (100% over 38bp)
CTTCTGGCGCCAAGCGCCCGGCCGCGAGATCGGAAGAGCACACGTCTGAA401660.11415982267170423Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGATTTAGCTCAGCGGTAGAGCAGCTGGAGATCGGAAGAGCACACGTC400140.11372780820558613No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG397980.11311389291162885Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CATAATTTGTGGTAGTGGGGGACTGAGATCGGAAGAGCACACGTCTGAAC379470.10785297990646715Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGTTTGCCGTC376950.10713674539685034TruSeq Adapter, Index 3 (97% over 42bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)