FastQCFastQC Report
Tue 19 Sept 2023
SRR16913178_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16913178_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58993699
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length35-62
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1559558126.43601141199842No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGNNNN3466010.5875220673990963No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGNNNNN3313730.5617091411745515No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGNNNNNN1445400.24500921700129366No Hit
CTCTCGCTTCTGGCGCCAAGCGCCCGGCCGNNNNN1346270.2282057275303249No Hit
GCCGTGATCGTATAGTGGTTAGTACTNNNNNNNNN1328310.22516133460287005No Hit
CTCTCGCTTCTGGCGCCAAGCGCCCGGNNNNNNNN1291330.21889286854177425No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTC1252290.21227521264601495TruSeq Adapter, Index 1 (100% over 50bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGNNN981520.16637709054317817No Hit
ACGAATTTTTGAGCGGGTAAAGGTCGCCCTCAAGN829870.1406709553845742No Hit
TGCATGGTTGTCGTCAGCTCGGGNNNNNNNNNNNN798100.13528563448784592No Hit
AAAACGTGATTCGTGATGCTGTGACTTACACGNNN755030.1279848547893225No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGNNNNNN694070.1176515478373377No Hit
CCTCTCGGCGCCCCCTCGATGCTCTNNNNNNNNNN683940.1159344153008612No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCG676840.11473089693867136No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCNNNNN634820.10760810235004928No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGNNNNNNN622380.10549940257178993No Hit
AGGGGCTCTCGCTTCTGGCGCCANNNNNNNNNNNN622030.10544007420182282No Hit
ACGGTCGAACTTGACTATCTAGNNNNNNNNNNNNN605690.10267028687250142No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)