FastQCFastQC Report
Tue 19 Sept 2023
SRR16913179_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16913179_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51789775
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length35-62
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1773822334.25043456937205No Hit
ACGAATTTTTGAGCGGGTAAAGGTCGCCCTCAAGN2097590.40502010290641344No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGNNNNN2004610.38706675207606134No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTC1983120.382917284348117TruSeq Adapter, Index 2 (100% over 50bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGNNNN1796570.34689666058599405No Hit
CTCTCGCTTCTGGCGCCAAGCGCCCGGCCGNNNNN1245230.2404393531348611No Hit
CTCTCGCTTCTGGCGCCAAGCGCCCGGNNNNNNNN1065400.20571628279906604No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGCCGTC1059610.20459830149870314TruSeq Adapter, Index 2 (98% over 50bp)
AGGGGCTCTCGCTTCTGGCGCCANNNNNNNNNNNN1008290.19468900955835394No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGNNNNNN929300.17943696414977667No Hit
ACGGTCGAACTTGACTATCTAGNNNNNNNNNNNNN880620.1700374253411991No Hit
CCTCTCGGCGCCCCCTCGATGCTCTNNNNNNNNNN839200.16203970764499362No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGNNN776620.14995624136231525No Hit
CGTAGAACCTTACCCACTCTTGACATCTTCTGNNN747910.14441267605429836No Hit
ACGAATTTTTGAGCGGGTAAAGGTCGCCCTCANNN716030.1382570208115405No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCG706590.1364342671888418No Hit
GCCGTGATCGTATAGTGGTTAGTACTNNNNNNNNN698990.13496679605192338No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGNNNNNN661770.12778004924717282No Hit
TGCATGGTTGTCGTCAGCTCGTGNNNNNNNNNNNN613090.11838051043859527No Hit
TTCGTGCTGTAAGACCTAAAGTTCTTATGNNNNNN568510.1097726336907237No Hit
CTCAGGATGAACGCTCGCTGTGTGCCTAATACATG545830.1053933908768671No Hit
AAAACGTGATTCGTGATGCTGTGACTTACACGNNN544530.10514237607713106No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)