FastQCFastQC Report
Tue 19 Sept 2023
SRR16913180_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16913180_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60369087
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length35-62
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2093893834.68486777015528No Hit
ACGAATTTTTGAGCGGGTAAAGGTCGCCCTCAAGN2462500.4079074444177034No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTC2261830.3746669218303732TruSeq Adapter, Index 3 (100% over 50bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGNNNNN2205410.36532107898202937No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGNNNN2179490.3610274907752042No Hit
AGGGGCTCTCGCTTCTGGCGCCANNNNNNNNNNNN1519980.2517811806562521No Hit
CTCTCGCTTCTGGCGCCAAGCGCCCGGCCGNNNNN1199130.19863311830440633No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGNNNNNN1108280.18358402538040702No Hit
CGTAGAACCTTACCCACTCTTGACATCTTCTGNNN1096010.181551528185278No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATGCCGTC1066020.17658375386727315TruSeq Adapter, Index 3 (98% over 50bp)
CTCAGGATGAACGCTCGCTGTGTGCCTAATACATG1039740.1722305324909088No Hit
CTCTCGCTTCTGGCGCCAAGCGCCCGGNNNNNNNN960080.15903503725342077No Hit
ACGAATTTTTGAGCGGGTAAAGGTCGCCCTCANNN834150.13817502325320904No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCG830970.1376482635889458No Hit
CCTCTCGGCGCCCCCTCGATGCTCTNNNNNNNNNN829730.13744286044942172No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGNNN782110.12955471730092588No Hit
ACGGTCGAACTTGACTATCTAGNNNNNNNNNNNNN779500.12912237682176642No Hit
GCCGTGATCGTATAGTGGTTAGTACTNNNNNNNNN772440.1279529041080247No Hit
AAAACGTGATTCGTGATGCTGTGACTTACACGNNN690300.11434660259148857No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)