FastQCFastQC Report
Tue 19 Sept 2023
SRR16913181_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16913181_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45869303
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length35-62
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1342351829.264708905648728No Hit
TGCATGGTTGTCGTCAGCTCGTGNNNNNNNNNNNN2229360.48602438977544526No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGNNNN2225210.48511964526690105No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGNNNNN2207700.48130227747301935No Hit
CTCTCGCTTCTGGCGCCAAGCGCCCGGNNNNNNNN1469660.32040164203061905No Hit
CTCTCGCTTCTGGCGCCAAGCGCCCGGCCGNNNNN1098480.239480421143526No Hit
TTCTGGGCTGTAGTGCGCTATGNNNNNNNNNNNNN915010.19948199343687434No Hit
TGCATGGTTGTCGTCAGCTCGGGNNNNNNNNNNNN840870.18331867828905096No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGNNN785470.17124088412679828No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGNNNNNN741610.1616789337304733No Hit
CCTCTCGGCGCCCCCTCGATGCTCTNNNNNNNNNN650090.1417265921830118No Hit
GCCGTGATCGTATAGTGGTTAGTACTNNNNNNNNN640850.13971217308447No Hit
CTTCTGGCGCCAAGCGCCCGGCCGCGNNNNNNNNN611220.13325251530418938No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGNNNNNN609750.1329320395385123No Hit
AAAACGTGATTCGTGATGCTGTGACTTACACGNNN583100.12712205371858384No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCNNNNN563850.12292534726328846No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTC540160.11776067318921327TruSeq Adapter, Index 4 (100% over 50bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGNNNNNNN527120.11491781333585994No Hit
CCGATTTAGCTCAGCGGTAGAGCAGCTGGCNNNNN521280.11364463070214954No Hit
CGTAGAACCTTACCCACTCTTGACATCTTCTGNNN495830.10809625775216163No Hit
TAAAAAGGGGTTGTAGGACATCNNNNNNNNNNNNN459920.1002674926191924No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)