FastQCFastQC Report
Tue 19 Sept 2023
SRR16913182_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16913182_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68934762
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length35-62
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2119416530.74525012503851No Hit
TGCATGGTTGTCGTCAGCTCGTGNNNNNNNNNNNN4518980.6555444406988741No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGNNNN2456210.3563093465093852No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGNNNNN2269740.3292591334398166No Hit
CTCTCGCTTCTGGCGCCAAGCGCCCGGNNNNNNNN1935080.2807117837006531No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTC1887580.2738212108427966TruSeq Adapter, Index 5 (100% over 50bp)
TTCTGGGCTGTAGTGCGCTATGNNNNNNNNNNNNN1462590.21217016749836606No Hit
CTCTCGCTTCTGGCGCCAAGCGCCCGGCCGNNNNN1254370.1819647973833579No Hit
ACGAATTTTTGAGCGGGTAAAGGTCGCCCTCAAGN1179770.17114297137922954No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGNNN1153860.1673843452161335No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGNNNNNN1074810.15591698133374277No Hit
CTTCTGGCGCCAAGCGCCCGGCCGCGNNNNNNNNN983280.14263921009838257No Hit
CCTCTCGGCGCCCCCTCGATGCTCTNNNNNNNNNN944170.13696573000426113No Hit
AAAACGTGATTCGTGATGCTGTGACTTACACGNNN943500.13686853666079243No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGTATGCCGTC882620.1280369982273965TruSeq Adapter, Index 5 (98% over 50bp)
CTTCTGGCGCCAAGCGCCCGGCCNNNNNNNNNNNN829970.12039934220705657No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGNNNNNN828010.12011501541123765No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGNNNNNNN826760.11993368454655721No Hit
AGGGGCTCTCGCTTCTGGCGCCANNNNNNNNNNNN824590.11961889416547199No Hit
CGTAGAACCTTACCCACTCTTGACATCTTCTGNNN755340.10957316426217587No Hit
ACGGTCGAACTTGACTATCTAGNNNNNNNNNNNNN740550.10742765747127697No Hit
AAAAGATTGATCGCCGTTCTGGTAAAAAGNNNNNN710390.10305250636826743No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCNNNNN709650.10294515849637663No Hit
GCCGTGATCGTATAGTGGTTAGTACTNNNNNNNNN693880.10065748830756825No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)