FastQCFastQC Report
Tue 19 Sept 2023
SRR16913183_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16913183_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73851988
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length35-62
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2398057632.471131311996636No Hit
TGCATGGTTGTCGTCAGCTCGTGNNNNNNNNNNNN5009620.6783324505766859No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGNNNNN2859040.38713108169816635No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGNNNN2837020.38414944226010544No Hit
TTCTGGGCTGTAGTGCGCTATGNNNNNNNNNNNNN2257910.30573449153460835No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTC2038640.2760440247051982TruSeq Adapter, Index 6 (100% over 50bp)
CTCTCGCTTCTGGCGCCAAGCGCCCGGNNNNNNNN1926290.26083116408457413No Hit
ACGAATTTTTGAGCGGGTAAAGGTCGCCCTCAAGN1538640.20834104018973734No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGNNN1313370.1778381375461416No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGNNNNNN1167780.15812438251492972No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGNNNNNN1102170.14924039688681096No Hit
CTCTCGCTTCTGGCGCCAAGCGCCCGGCCGNNNNN1085340.14696151442802055No Hit
CCTCTCGGCGCCCCCTCGATGCTCTNNNNNNNNNN1016760.13767537307187994No Hit
CTTCTGGCGCCAAGCGCCCGGCCGCGNNNNNNNNN962970.13039188599770665No Hit
GCCGTGATCGTATAGTGGTTAGTACTNNNNNNNNN944520.12789364586908616No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGNNNNNNN895460.12125062902842913No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCGCGTATGCCGTC893110.12093242500120648TruSeq Adapter, Index 6 (98% over 50bp)
AAAACGTGATTCGTGATGCTGTGACTTACACGNNN880150.11917756364256571No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCNNNNN787250.1065983491195931No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)