FastQCFastQC Report
Wed 20 Sept 2023
SRR16955384_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16955384_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81788555
Total Bases12.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGATATAGTTTAATGGTAGAACTACGGTCTGGAATTCTCGGGTGCCAA59505707.275553407197376No Hit
GGGTGGTTAGCTCAGCTGGCAGAGCAACGGACTGGAATTCTCGGGTGCCA49800866.088976630043164No Hit
GCGGGTGTAGTTTAGTGGTAAAACCACAGCCTGGAATTCTCGGGTGCCAA32828354.013807310815064No Hit
GCGGAAGTAGTTCAGTGGTAGAACATCACCTTTGGAATTCTCGGGTGCCA21110272.5810787340600405No Hit
CGCGGGATGGAGCAGTTCGGTAGCTCGTCGGGCTGGAATTCTCGGGTGCC19643192.4017040036958717No Hit
TCCGGTTTAGCTCAGTTGGTAGAGCACCTGACTGGAATTCTCGGGTGCCA18552162.2683075890019087No Hit
GGTTCCATGGTCTAGTTGGTTAGGACGCCTGCCTGGAATTCTCGGGTGCC12521501.5309599246496042No Hit
GCGGGCATGGCGGAATTGGTAGACGCGCTGGAATTCTCGGGTGCCAAGGA11594321.4175968752596253RNA PCR Primer, Index 1 (100% over 22bp)
AGGACAAGCTGTGATGGGGAGCGATGGAATTCTCGGGTGCCAAGGAACTC10185831.2453857388726333RNA PCR Primer, Index 1 (100% over 26bp)
GCACCCATAGCGCAACTGGATAGAGTGTCTGATGGAATTCTCGGGTGCCA10007341.2235623920730718No Hit
GCGGAAGTAGTTCAGTGGTAGAACATCACCTTGGAATTCTCGGGTGCCAA9770291.1945791192912014No Hit
GCCGACTTAGCTCAGTTGGTAGAGCATCTGTCTGGAATTCTCGGGTGCCA9521781.1641946724697605No Hit
ACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTGGAATTCTCGGGTGCCA9209991.126073201806781No Hit
GCACCCATAGCGCAATTGGATAGAGTGTCTGACTGGAATTCTCGGGTGCC8980531.098017931726511No Hit
GCGGTCGTAGTTTAGTGGTAAAACTCCAGCTGGAATTCTCGGGTGCCAAG8440641.032007473417277No Hit
GCCGTCTTAGCTCAGTCGGTAGAGCGCATCCTGGAATTCTCGGGTGCCAA7945190.9714305381724863No Hit
GCACCCATAGCGCAACTGGATAGAGTGTCTGTGGAATTCTCGGGTGCCAA7763590.9492269425716104No Hit
TGCTTCTCGGGGTTGTAGGACTGGACTGGAATTCTCGGGTGCCAAGGAAC7697860.9411903658158529RNA PCR Primer, Index 1 (100% over 24bp)
GCACCCATAGCGCAATTGGATAGAGTGTCTGATGGAATTCTCGGGTGCCA7682070.9392597778503362No Hit
GCCGACTTAGCTCAGTTGGTAGAGCATCTGTTGGAATTCTCGGGTGCCAA7360790.8999779981441169No Hit
AGGATGAGGTCGTCGGTTCAAGCCCGATAGACGGCTGGAATTCTCGGGTG7354960.8992651844747716No Hit
GCACCCATAGCGCAACTGGATAGAGTGTCTGACTGGAATTCTCGGGTGCC7118240.870322259636449No Hit
GCGGAAGTAGTTCAGTGGTAGAACATCACCTTGCCTGGAATTCTCGGGTG6557580.8017723262136126No Hit
GCCGTCTTAGCTCAGTCGGTAGAGCGCATCCATGGAATTCTCGGGTGCCA6210240.7593042816320694No Hit
TGCTCATGGAAGTTCGAGTCTTCTTGCCCGCACCTGGAATTCTCGGGTGC5980960.7312710195210076No Hit
AACGATGAATGCTAGGTGTTGGAGGGTGGAATTCTCGGGTGCCAAGGAAC5304290.6485369499436687RNA PCR Primer, Index 1 (100% over 24bp)
ACGATGAATGCTAGGTGTTGGAGGGTGGAATTCTCGGGTGCCAAGGAACT5295850.6475050207208087RNA PCR Primer, Index 1 (100% over 25bp)
CACATGTAGATCCTGAGTGATCGCGGTCTGCTGGAATTCTCGGGTGCCAA4422820.5407627020675447No Hit
GGTTCCATGGTCTAGTTGGTTAGGACGCCTGCCTGTGGAATTCTCGGGTG4231870.5174159147328131No Hit
GCACCCATAGCGCAATTGGATAGAGTGTCTGTGGAATTCTCGGGTGCCAA4181320.5112353433802566No Hit
TGCCCAGTAGTTCAGTGGTAGAATGCTGGAATTCTCGGGTGCCAAGGAAC3250920.3974785958744472RNA PCR Primer, Index 1 (100% over 24bp)
AAATTCCGTCGGAATCCGGGAGGACCTGGAATTCTCGGGTGCCAAGGAAC3196250.3907942865600205RNA PCR Primer, Index 1 (100% over 24bp)
GCGGAAGTAGTTCAGTGGTAGAACATCACCTTGTGGAATTCTCGGGTGCC3124400.3820094388511938No Hit
CACATGTAGATCCTGAGTGATCGCGGTCTGCATGGAATTCTCGGGTGCCA3065870.37485318086375286No Hit
TGCCCAGTAGTTCAGTGGTAGAATGCTTGACTGGAATTCTCGGGTGCCAA3053280.3733138456841547No Hit
AGCCGTCTAAGGTGGGACAAATGATTAGGGTGTGGAATTCTCGGGTGCCA3038020.3714480589613058No Hit
ACCGGGATGGACGTTCCGCTGGTGTACCAGTTGGAATTCTCGGGTGCCAA2962610.36222794252814466No Hit
GGTTCCATGGTCTAGTTGGTTAGGACGCCTGCCTTGGAATTCTCGGGTGC2764600.33801795373448035No Hit
GGCTCGGTAGCTCAGTCGGTAGAGCAATGCTGGAATTCTCGGGTGCCAAG2692720.3292294380307856No Hit
GGCTCGGTAGCTCAGTCGGTAGAGCAATGCATGGAATTCTCGGGTGCCAA2402220.2937110210591201No Hit
TGACCGTGGGGGTTCGAGTCCCTTCACCCGCACCTGGAATTCTCGGGTGC2298390.28101609082102014No Hit
GCGGTCGTAGTTTAGTGGTAAAACTCCAGCTTGGAATTCTCGGGTGCCAA2260480.2763809679728417No Hit
GCGGAAGTAGTTCAGTGGTAGAACATCACCTGGAATTCTCGGGTGCCAAG2246140.27462766642594433No Hit
TCATGGAAGTTCGAGTCTTCTTGCCCGCACCTGGAATTCTCGGGTGCCAA2154550.2634292780939827No Hit
ACCGTGGGGGTTCGAGTCCCTTCACCCGCACCTGGAATTCTCGGGTGCCA2084660.2548840727165311No Hit
ACCTGCACACACGAAACTTTGTTTAGTGGAATTCTCGGGTGCCAAGGAAC2062880.2522211084423731RNA PCR Primer, Index 1 (100% over 24bp)
TCCGGTTTAGCTCAGTTGGTAGAGCACCTGACTTGGAATTCTCGGGTGCC1973760.2413247183545424No Hit
TAAAGGAGGAAATTTTAGCATGACTGTTAAGATGGAATTCTCGGGTGCCA1909460.23346298268749213No Hit
ACACACTGGAGGACCGAACCCATGTGGAATTCTCGGGTGCCAAGGAACTC1695380.20728817130954325RNA PCR Primer, Index 1 (100% over 26bp)
ACGATGAATGCTAGGTGTTGGAGGGTTGGAATTCTCGGGTGCCAAGGAAC1672320.20446870592101793RNA PCR Primer, Index 1 (100% over 24bp)
AGCTTGTTGGCGGCAGACGCGGGGAACTGATGGAATTCTCGGGTGCCAAG1594210.19491846994973808No Hit
ACATGTAGATCCTGAGTGATCGCGGTCTGCTGGAATTCTCGGGTGCCAAG1542600.18860829611184596No Hit
TTGACCGTGGGGGTTCGAGTCCCTTCACCCGCACCTGGAATTCTCGGGTG1529890.18705428895277584No Hit
AAAGGAGGAAATTTTAGCATGACTGTTAAGATGGAATTCTCGGGTGCCAA1527260.18673272806934907No Hit
GCGGGTGTAGTTTAGTGGTAAAACCACAGCTGGAATTCTCGGGTGCCAAG1519570.18579249871818862No Hit
AAGGCTCGTCCTCCCAGGGTTAGTCGGGATGGAATTCTCGGGTGCCAAGG1517570.18554796572698956Illumina Small RNA Adapter 2 (100% over 21bp)
GCGGGCATGGCGGAATTGGTAGACGCGCAAGATTTGGAATTCTCGGGTGC1500000.1833997433993057No Hit
TGACCGTACCGGTTCGATCCCGGTCCTCGGCACCTGGAATTCTCGGGTGC1412530.17270509302921416No Hit
AACGATGAATGCTAGGTGTTGGAGGGTTGGAATTCTCGGGTGCCAAGGAA1309770.16014098794140574RNA PCR Primer, Index 1 (100% over 23bp)
GCGGAAGTAGTTCAGTGGTAGAACATCACCTTGCTGGAATTCTCGGGTGC1300570.15901613618189No Hit
AACAGGGGGTCGAAGGTTCGAATCCTTTAGTCGGCACCATGGAATTCTCG1294900.15832288515184062No Hit
GCGGGCATGGCGGAATTGGTAGACGCGCAAGATTGGAATTCTCGGGTGCC1224580.14972510518128115No Hit
AGGACAAGCTGTGATGGGGAGCGTGGAATTCTCGGGTGCCAAGGAACTCC1199840.14670023208014862RNA PCR Primer, Index 1 (100% over 27bp)
TGGGTAGTAGCGAAGTGGTAGCGCAGTGGACTGGAATTCTCGGGTGCCAA1197500.14641412848044572No Hit
ACCATTGATCTTACTCCGGGAGTCAGACAGTGGAATTCTCGGGTGCCAAG1195670.14619038079349855No Hit
TGGCGGGAGACAGTGTCAGGTAGGCAGTGGAATTCTCGGGTGCCAAGGAA1181670.14447864985510503RNA PCR Primer, Index 1 (100% over 23bp)
GCGGGTGTGGCGGAACTGGCAGACGCGCTAGATTTGGAATTCTCGGGTGC1171560.1432425355845937No Hit
GGATGAGGTCGTCGGTTCAAGCCCGATAGACGGCTGGAATTCTCGGGTGC1170760.14314472238811407No Hit
AAGGACAAGCTGTGATGGGGAGCGATGGAATTCTCGGGTGCCAAGGAACT1152600.1409243628280265RNA PCR Primer, Index 1 (100% over 25bp)
GGGTGGTTAGCTCAGCTGGCAGAGCAACGGATGGAATTCTCGGGTGCCAA1100260.1345249344483467No Hit
TTGCTTCTCGGGGTTGTAGGACTGGACTGGAATTCTCGGGTGCCAAGGAA1097440.13418014293075603RNA PCR Primer, Index 1 (100% over 23bp)
AACCTGCACACACGAAACTTTGTTTAGTGGAATTCTCGGGTGCCAAGGAA1080450.1321028351705199RNA PCR Primer, Index 1 (100% over 23bp)
GGGATATAGTTTAATGGTAGAACTACGGTCTGGAATTCTCGGGTGCCAAG1058030.12936162033917825No Hit
AGGCTCGTCCTCCCAGGGTTAGTCGGGATGGAATTCTCGGGTGCCAAGGA1055900.12910119270355125RNA PCR Primer, Index 1 (100% over 22bp)
ACCACGGTGAGTTCGTAACGATCGGCGTGGAATTCTCGGGTGCCAAGGAA1054640.12894713691909584RNA PCR Primer, Index 1 (100% over 23bp)
AACAGGGGGTCGAAGGTTCGAATCCTTTAGTCGGCACCTGGAATTCTCGG972180.11886504169195801No Hit
TAAGGACAAGCTGTGATGGGGAGCGATGGAATTCTCGGGTGCCAAGGAAC949300.11606758427264059RNA PCR Primer, Index 1 (100% over 24bp)
GCGGAAGTAGTTCAGTGGTAGAACTGGAATTCTCGGGTGCCAAGGAACTC924870.1130806137851439RNA PCR Primer, Index 1 (100% over 26bp)
GAGCCGTTAGCTCAGTTGGTAGAGCAACTGACTGGAATTCTCGGGTGCCA923060.11285931142810875No Hit
AAGCATAATACCGAAACTGTGGGTGCACCCGTGGAATTCTCGGGTGCCAA919490.11242282003881839No Hit
AAACACACTGGAGGACCGAACCCATGTGGAATTCTCGGGTGCCAAGGAAC876600.10717881004255424RNA PCR Primer, Index 1 (100% over 24bp)
CGCGGGATGGAGCAGTTCGGTAGCTCGTCGGGCTTGGAATTCTCGGGTGC851330.10408913569875393No Hit
GGGTCCATAGCTCAGTTGGGAGAGCGCCTGTGGAATTCTCGGGTGCCAAG826870.10109849721638928No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)