FastQCFastQC Report
Tue 19 Sept 2023
SRR16955385_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16955385_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81788555
Total Bases12.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCGTAGTTCTACCATTAAACTATATCCCTGATCGTCGGACTGTAGAAC55561396.793296445939166No Hit
GTCCGTTGCTCTGCCAGCTGAGCTAACCACCCGATCGTCGGACTGTAGAA46318395.663187227112644No Hit
GGCTGTGGTTTTACCACTAAACTACACCCGCGATCGTCGGACTGTAGAAC30067563.6762551924288185No Hit
AAGGTGATGTTCTACCACTGAACTACTTCCGCGATCGTCGGACTGTAGAA19568152.392529125866082No Hit
GCCCGACGAGCTACCGAACTGCTCCATCCCGCGGATCGTCGGACTGTAGA18523352.2647850912636858No Hit
GTCAGGTGCTCTACCAACTGAGCTAAACCGGAGATCGTCGGACTGTAGAA17198372.1027844299242116No Hit
GGCAGGCGTCCTAACCAACTAGACCATGGAACCGATCGTCGGACTGTAGA11425681.3969778534417192No Hit
GCGCGTCTACCAATTCCGCCATGCCCGCGATCGTCGGACTGTAGAACTCT10761031.315713427141487Illumina DpnII expression Sequencing Primer (100% over 22bp)
TCGCTCCCCATCACAGCTTGTCCTGATCGTCGGACTGTAGAACTCTGAAC9320851.1396276655089455Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGGTGATGTTCTACCACTGAACTACTTCCGCGATCGTCGGACTGTAGAAC9081061.1103093825291326No Hit
TCAGACACTCTATCCAGTTGCGCTATGGGTGCGATCGTCGGACTGTAGAA9025381.1035015840541502No Hit
GACAGATGCTCTACCAACTGAGCTAAGTCGGCGATCGTCGGACTGTAGAA8664471.0593743831273215No Hit
CCACATGCTCCACCGCTTGTGCGGGCCCCCGTGATCGTCGGACTGTAGAA8207761.0035340519220568No Hit
GTCAGACACTCTATCCAATTGCGCTATGGGTGCGATCGTCGGACTGTAGA8132470.9943286074683677No Hit
GCTGGAGTTTTACCACTAAACTACGACCGCGATCGTCGGACTGTAGAACT7866550.9618155009585387No Hit
GGATGCGCTCTACCGACTGAGCTAAGACGGCGATCGTCGGACTGTAGAAC7399250.9046803675648751No Hit
GTCCAGTCCTACAACCCCGAGAAGCAGATCGTCGGACTGTAGAACTCTGA7137880.872723573610024Illumina DpnII expression Sequencing Primer (100% over 24bp)
CAGACACTCTATCCAGTTGCGCTATGGGTGCGATCGTCGGACTGTAGAAC7013930.8575686414804613No Hit
GCCGTCTATCGGGCTTGAACCGACGACCTCATCCTGATCGTCGGACTGTA6929260.8472163372980487No Hit
TCAGACACTCTATCCAATTGCGCTATGGGTGCGATCGTCGGACTGTAGAA6866160.8395013214257178No Hit
ACAGATGCTCTACCAACTGAGCTAAGTCGGCGATCGTCGGACTGTAGAAC6698500.8190021207734994No Hit
GTCAGACACTCTATCCAGTTGCGCTATGGGTGCGATCGTCGGACTGTAGA6503520.7951625994615017No Hit
GGCAAGGTGATGTTCTACCACTGAACTACTTCCGCGATCGTCGGACTGTA6125050.7488883988719448No Hit
TGGATGCGCTCTACCGACTGAGCTAAGACGGCGATCGTCGGACTGTAGAA5808590.7101959436745153No Hit
GGTGCGGGCAAGAAGACTCGAACTTCCATGAGCAGATCGTCGGACTGTAG5558260.6795889718310832No Hit
CCCTCCAACACCTAGCATTCATCGTTGATCGTCGGACTGTAGAACTCTGA4755150.581395526550139Illumina DpnII expression Sequencing Primer (100% over 24bp)
CCCTCCAACACCTAGCATTCATCGTGATCGTCGGACTGTAGAACTCTGAA4664920.5703634206521927Illumina DpnII expression Sequencing Primer (100% over 25bp)
GCAGACCGCGATCACTCAGGATCTACATGTGGATCGTCGGACTGTAGAAC4089670.500029619058559No Hit
CAGGCAGGCGTCCTAACCAACTAGACCATGGAACCGATCGTCGGACTGTA3901680.47704474055080204No Hit
CAGACACTCTATCCAATTGCGCTATGGGTGCGATCGTCGGACTGTAGAAC3735850.45676928758553076No Hit
GCATTCTACCACTGAACTACTGGGCAGATCGTCGGACTGTAGAACTCTGA3001770.36701589849582256Illumina DpnII expression Sequencing Primer (100% over 24bp)
GGTCCTCCCGGATTCCGACGGAATTTGATCGTCGGACTGTAGAACTCTGA2999430.3667297948961196Illumina DpnII expression Sequencing Primer (100% over 24bp)
CAAGGTGATGTTCTACCACTGAACTACTTCCGCGATCGTCGGACTGTAGA2938520.3592825426491518No Hit
GTCAAGCATTCTACCACTGAACTACTGGGCAGATCGTCGGACTGTAGAAC2858180.349459652392685No Hit
TGCAGACCGCGATCACTCAGGATCTACATGTGGATCGTCGGACTGTAGAA2854560.3490170476786147No Hit
CACCCTAATCATTTGTCCCACCTTAGACGGCTGATCGTCGGACTGTAGAA2799640.3423021717402881No Hit
GCATTGCTCTACCGACTGAGCTACCGAGCCGATCGTCGGACTGTAGAACT2528610.30916428343794555No Hit
AGGCAGGCGTCCTAACCAACTAGACCATGGAACCGATCGTCGGACTGTAG2512900.30724347679207686No Hit
ACTGGTACACCAGCGGAACGTCCATCCCGGTGATCGTCGGACTGTAGAAC2508970.30676296946437065No Hit
TGCATTGCTCTACCGACTGAGCTACCGAGCCGATCGTCGGACTGTAGAAC2271020.2776696568364608No Hit
GGTGATGTTCTACCACTGAACTACTTCCGCGATCGTCGGACTGTAGAACT2091460.25571548488660795No Hit
AGCTGGAGTTTTACCACTAAACTACGACCGCGATCGTCGGACTGTAGAAC2087380.2552166375845618No Hit
GGTGCGGGTGAAGGGACTCGAACCCCCACGGTCAGATCGTCGGACTGTAG2058500.2516855811916472No Hit
GGTGCGGGCAAGAAGACTCGAACTTCCATGAGATCGTCGGACTGTAGAAC1994400.24384829882371684No Hit
CTAAACAAAGTTTCGTGTGTGCAGGTGATCGTCGGACTGTAGAACTCTGA1888980.23095896485761364Illumina DpnII expression Sequencing Primer (100% over 24bp)
GGTGCGGGTGAAGGGACTCGAACCCCCACGGTGATCGTCGGACTGTAGAA1846360.22574796681516135No Hit
AGTCAGGTGCTCTACCAACTGAGCTAAACCGGAGATCGTCGGACTGTAGA1839030.22485175340241675No Hit
TCTTAACAGTCATGCTAAAATTTCCTCCTTTAGATCGTCGGACTGTAGAA1775730.21711228423096607No Hit
CATGGGTTCGGTCCTCCAGTGTGTGATCGTCGGACTGTAGAACTCTGAAC1537250.18795417036038845Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACCCTCCAACACCTAGCATTCATCGTGATCGTCGGACTGTAGAACTCTGA1493510.1826062338428647Illumina DpnII expression Sequencing Primer (100% over 24bp)
TCAGTTCCCCGCGTCTGCCGCCAACAAGCTGATCGTCGGACTGTAGAACT1477290.18062307128424018No Hit
GCAGACCGCGATCACTCAGGATCTACATGTGATCGTCGGACTGTAGAACT1415240.17303643523228893No Hit
GCTGTGGTTTTACCACTAAACTACACCCGCGATCGTCGGACTGTAGAACT1401650.1713748335570912No Hit
AATCTTGCGCGTCTACCAATTCCGCCATGCCCGCGATCGTCGGACTGTAG1395440.17061555861941807No Hit
GGTGCGGGTGAAGGGACTCGAACCCCCACGGTCAAGATCGTCGGACTGTA1393180.17033923633936313No Hit
TCTTAACAGTCATGCTAAAATTTCCTCCTTTGATCGTCGGACTGTAGAAC1388200.16973034919127744No Hit
TCCCGACTAACCCTGGGAGGACGAGCCTTGATCGTCGGACTGTAGAACTC1365900.16700380633940776Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGTGCCGAGGACCGGGATCGAACCGGTACGGTCAGATCGTCGGACTGTAG1281340.15666495147151088No Hit
TGGTGCCGACTAAAGGATTCGAACCTTCGACCCCCTGTTGATCGTCGGAC1212580.14825790723408672No Hit
GCAAGGTGATGTTCTACCACTGAACTACTTCCGCGATCGTCGGACTGTAG1210730.14803171421722758No Hit
ACCCTCCAACACCTAGCATTCATCGTTGATCGTCGGACTGTAGAACTCTG1184720.14485156266668364Illumina DpnII expression Sequencing Primer (100% over 23bp)
ATCTTGCGCGTCTACCAATTCCGCCATGCCCGCGATCGTCGGACTGTAGA1153400.14102217602450612No Hit
GTCCACTGCGCTACCACTTCGCTACTACCCAGATCGTCGGACTGTAGAAC1114330.1362452240414322No Hit
CGCTCCCCATCACAGCTTGTCCTGATCGTCGGACTGTAGAACTCTGAACG1109640.13567179417707037Illumina Small RNA Adapter 1 (100% over 26bp)
AATCTAGCGCGTCTGCCAGTTCCGCCACACCCGCGATCGTCGGACTGTAG1078960.13192065809207656No Hit
CTGCCTACCTGACACTGTCTCCCGCCAGATCGTCGGACTGTAGAACTCTG1073970.13131054827903488Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCGCTCCCCATCACAGCTTGTCCTTGATCGTCGGACTGTAGAACTCTGAA1046380.12793721566544367Illumina DpnII expression Sequencing Primer (100% over 25bp)
TCCGTTGCTCTGCCAGCTGAGCTAACCACCCGATCGTCGGACTGTAGAAC1027020.1255701363106366No Hit
GTCCAGTCCTACAACCCCGAGAAGCAAGATCGTCGGACTGTAGAACTCTG1021570.12490378390961913Illumina DpnII expression Sequencing Primer (100% over 23bp)
GACCGTAGTTCTACCATTAAACTATATCCCTATCGTCGGACTGTAGAACT1013290.12389141732605498No Hit
CGCCGATCGTTACGAACTCACCGTGGTGATCGTCGGACTGTAGAACTCTG999000.12214422910393759Illumina DpnII expression Sequencing Primer (100% over 23bp)
CTAAACAAAGTTTCGTGTGTGCAGGTTGATCGTCGGACTGTAGAACTCTG969250.11850680085985137Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCCCGACTAACCCTGGGAGGACGAGCCTGATCGTCGGACTGTAGAACTCT959040.11725845993978008Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCCGTCTATCGGGCTTGAACCGACGACCTCATCCGATCGTCGGACTGTAG951870.1163818091663314No Hit
GGTGCCGACTAAAGGATTCGAACCTTCGACCCCCTGTTGATCGTCGGACT909260.11117203378883513No Hit
TCGCTCCCCATCACAGCTTGTCCTTAGATCGTCGGACTGTAGAACTCTGA887650.10852985481892913Illumina DpnII expression Sequencing Primer (100% over 24bp)
CTGTCTGACTCCCGGAGTAAGATCAATGGTGATCGTCGGACTGTAGAACT886590.10840025233359361No Hit
GTTCTACCACTGAACTACTTCCGCGATCGTCGGACTGTAGAACTCTGAAC857630.10485941462103103Illumina DpnII expression Sequencing Primer (100% over 26bp)
GTCAGTTGCTCTACCAACTGAGCTAACGGCTCGATCGTCGGACTGTAGAA838320.10249844859100397No Hit
CGGGTGCACCCACAGTTTCGGTATTATGCTTGATCGTCGGACTGTAGAAC830650.10156066456975552No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)