FastQCFastQC Report
Tue 19 Sept 2023
SRR16955386_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16955386_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61136412
Total Bases9.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGATATAGTTTAATGGTAGAACTACGGTCTGGAATTCTCGGGTGCCAA46682657.635817751293615No Hit
GGGTGGTTAGCTCAGCTGGCAGAGCAACGGACTGGAATTCTCGGGTGCCA23765893.887354396918157No Hit
GCGGGTGTAGTTTAGTGGTAAAACCACAGCCTGGAATTCTCGGGTGCCAA19147543.131937150646001No Hit
CGCGGGATGGAGCAGTTCGGTAGCTCGTCGGGCTGGAATTCTCGGGTGCC17258372.822928175765369No Hit
GCGGGCATGGCGGAATTGGTAGACGCGCTGGAATTCTCGGGTGCCAAGGA14136942.312360103828141RNA PCR Primer, Index 1 (100% over 22bp)
AGGACAAGCTGTGATGGGGAGCGATGGAATTCTCGGGTGCCAAGGAACTC13431692.1970033177609443RNA PCR Primer, Index 1 (100% over 26bp)
GCACCCATAGCGCAACTGGATAGAGTGTCTGTGGAATTCTCGGGTGCCAA12605652.061889075204479No Hit
ACGATGAATGCTAGGTGTTGGAGGGTGGAATTCTCGGGTGCCAAGGAACT11812741.9321938618183871RNA PCR Primer, Index 1 (100% over 25bp)
GCGGAAGTAGTTCAGTGGTAGAACATCACCTTTGGAATTCTCGGGTGCCA10575941.7298921631187645No Hit
AACGATGAATGCTAGGTGTTGGAGGGTGGAATTCTCGGGTGCCAAGGAAC9966631.6302281527414464RNA PCR Primer, Index 1 (100% over 24bp)
GCGGTCGTAGTTTAGTGGTAAAACTCCAGCTGGAATTCTCGGGTGCCAAG9942181.626228899399592No Hit
TGCTTCTCGGGGTTGTAGGACTGGACTGGAATTCTCGGGTGCCAAGGAAC9449531.5456468070124887RNA PCR Primer, Index 1 (100% over 24bp)
GCACCCATAGCGCAACTGGATAGAGTGTCTGATGGAATTCTCGGGTGCCA8658471.4162541956174988No Hit
GCGGAAGTAGTTCAGTGGTAGAACATCACCTTGGAATTCTCGGGTGCCAA8517531.3932008309548816No Hit
GGTTCCATGGTCTAGTTGGTTAGGACGCCTGCCTGGAATTCTCGGGTGCC7739671.2659673256585617No Hit
GCACCCATAGCGCAATTGGATAGAGTGTCTGATGGAATTCTCGGGTGCCA6893041.1274852047254589No Hit
GCACCCATAGCGCAATTGGATAGAGTGTCTGTGGAATTCTCGGGTGCCAA6860531.122167588114265No Hit
AGGATGAGGTCGTCGGTTCAAGCCCGATAGACGGCTGGAATTCTCGGGTG6777991.108666632251824No Hit
GCCGTCTTAGCTCAGTCGGTAGAGCGCATCCTGGAATTCTCGGGTGCCAA6159661.0075272327070812No Hit
GCCGACTTAGCTCAGTTGGTAGAGCATCTGTTGGAATTCTCGGGTGCCAA5625890.9202191976853337No Hit
GGCTCGGTAGCTCAGTCGGTAGAGCAATGCTGGAATTCTCGGGTGCCAAG5319280.8700674157979699No Hit
GCACCCATAGCGCAATTGGATAGAGTGTCTGACTGGAATTCTCGGGTGCC4580630.7492474370265628No Hit
CACATGTAGATCCTGAGTGATCGCGGTCTGCTGGAATTCTCGGGTGCCAA4417520.7225677555300433No Hit
GCCGACTTAGCTCAGTTGGTAGAGCATCTGTCTGGAATTCTCGGGTGCCA3869840.6329844806725No Hit
GCGGAAGTAGTTCAGTGGTAGAACATCACCTGGAATTCTCGGGTGCCAAG3662000.5989883737370783No Hit
TCCGGTTTAGCTCAGTTGGTAGAGCACCTGACTGGAATTCTCGGGTGCCA3443910.5633156882023106No Hit
GCACCCATAGCGCAACTGGATAGAGTGTCTGACTGGAATTCTCGGGTGCC3434100.5617110798062536No Hit
AAATTCCGTCGGAATCCGGGAGGACCTGGAATTCTCGGGTGCCAAGGAAC3313780.5420305005795891RNA PCR Primer, Index 1 (100% over 24bp)
ACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTGGAATTCTCGGGTGCCA2919180.4774863137208641No Hit
TCATGGAAGTTCGAGTCTTCTTGCCCGCACCTGGAATTCTCGGGTGCCAA2672010.43705705202326894No Hit
ACCGGGATGGACGTTCCGCTGGTGTACCAGTTGGAATTCTCGGGTGCCAA2664330.43580084483858816No Hit
AGGACAAGCTGTGATGGGGAGCGTGGAATTCTCGGGTGCCAAGGAACTCC2643790.4324411448941426RNA PCR Primer, Index 1 (100% over 27bp)
TGCTCATGGAAGTTCGAGTCTTCTTGCCCGCACCTGGAATTCTCGGGTGC2563290.4192738690651326No Hit
GGTTCCATGGTCTAGTTGGTTAGGACGCCTGCCTGTGGAATTCTCGGGTG2232360.3651440977596134No Hit
CACATGTAGATCCTGAGTGATCGCGGTCTGCATGGAATTCTCGGGTGCCA2148410.35141251010936003No Hit
ACCTGCACACACGAAACTTTGTTTAGTGGAATTCTCGGGTGCCAAGGAAC2109280.3450120690759543RNA PCR Primer, Index 1 (100% over 24bp)
GCGGAAGTAGTTCAGTGGTAGAACATCACCTTGCCTGGAATTCTCGGGTG2009090.32862412664976154No Hit
ACACACTGGAGGACCGAACCCATGTGGAATTCTCGGGTGCCAAGGAACTC1870930.30602548281701586RNA PCR Primer, Index 1 (100% over 26bp)
GGCTCGGTAGCTCAGTCGGTAGAGCAATGCATGGAATTCTCGGGTGCCAA1773320.29005954749192675No Hit
AAGGCTCGTCCTCCCAGGGTTAGTCGGGATGGAATTCTCGGGTGCCAAGG1702370.27845435221157566Illumina Small RNA Adapter 2 (100% over 21bp)
AGCCGTCTAAGGTGGGACAAATGATTAGGGTGTGGAATTCTCGGGTGCCA1679840.27476915066589125No Hit
GCGGGTGTAGTTTAGTGGTAAAACCACAGCTGGAATTCTCGGGTGCCAAG1652020.2702186709943004No Hit
AGGCTCGTCCTCCCAGGGTTAGTCGGGATGGAATTCTCGGGTGCCAAGGA1586320.2594722110941021RNA PCR Primer, Index 1 (100% over 22bp)
AGCTTGTTGGCGGCAGACGCGGGGAACTGATGGAATTCTCGGGTGCCAAG1578660.2582192752822982No Hit
AACAGGGGGTCGAAGGTTCGAATCCTTTAGTCGGCACCATGGAATTCTCG1551060.2537047807123519No Hit
TGCCCAGTAGTTCAGTGGTAGAATGCTGGAATTCTCGGGTGCCAAGGAAC1439350.23543252750913812RNA PCR Primer, Index 1 (100% over 24bp)
ACCGTGGGGGTTCGAGTCCCTTCACCCGCACCTGGAATTCTCGGGTGCCA1416750.23173587615838495No Hit
ACATGTAGATCCTGAGTGATCGCGGTCTGCTGGAATTCTCGGGTGCCAAG1369220.22396145851673468No Hit
TGCTTCTCGGGGTTGTAGGACTGGTGGAATTCTCGGGTGCCAAGGAACTC1333590.21813350773676413RNA PCR Primer, Index 1 (100% over 26bp)
GCCGTCTTAGCTCAGTCGGTAGAGCGCATCCATGGAATTCTCGGGTGCCA1324440.21663685464564064No Hit
TGACCGTACCGGTTCGATCCCGGTCCTCGGCACCTGGAATTCTCGGGTGC1252720.20490571150953377No Hit
CGATGAATGCTAGGTGTTGGAGGGTGGAATTCTCGGGTGCCAAGGAACTC1209320.19780683236693708RNA PCR Primer, Index 1 (100% over 26bp)
GCGGTCGTAGTTTAGTGGTAAAACTCCAGCTTGGAATTCTCGGGTGCCAA1194470.19537783800593336No Hit
AACAGGGGGTCGAAGGTTCGAATCCTTTAGTCGGCACCTGGAATTCTCGG1163960.19038735868241663No Hit
GCGGGCATGGCGGAATTGGTAGACGCGTGGAATTCTCGGGTGCCAAGGAA1140030.1864731610353581RNA PCR Primer, Index 1 (100% over 23bp)
GGGTGGTTAGCTCAGCTGGCAGAGCAACGGATGGAATTCTCGGGTGCCAA1136250.1858548715616481No Hit
TCGCTGGGTAGCTATGAACGGAAGGGTGGAATTCTCGGGTGCCAAGGAAC1121930.1835125685818788RNA PCR Primer, Index 1 (100% over 24bp)
AGTCGAGCAGGGACGAAAGTCGGTGGAATTCTCGGGTGCCAAGGAACTCC1105720.18086112086525458RNA PCR Primer, Index 1 (100% over 27bp)
AGCGAGCGCAGGCGGTTTTTTAAGTCTGATGGAATTCTCGGGTGCCAAGG1098120.17961799917208096Illumina Small RNA Adapter 2 (100% over 21bp)
GGTTCCATGGTCTAGTTGGTTAGGACGCCTGCTGGAATTCTCGGGTGCCA1078730.17644640316804983No Hit
ACTCCGGGAGTCAGACAGTGAGCGTGGAATTCTCGGGTGCCAAGGAACTC1071770.17530796540693294RNA PCR Primer, Index 1 (100% over 26bp)
TTGCTTCTCGGGGTTGTAGGACTGGACTGGAATTCTCGGGTGCCAAGGAA1023550.167420685401034RNA PCR Primer, Index 1 (100% over 23bp)
ACGATGAATGCTAGGTGTTGGAGGGTTGGAATTCTCGGGTGCCAAGGAAC1016470.16626261940265646RNA PCR Primer, Index 1 (100% over 24bp)
AAGCATAATACCGAAACTGTGGGTGCACCCGTGGAATTCTCGGGTGCCAA1015130.1660434374199127No Hit
AACCGAGGGTCAGGTGTTCGAGTCACCTAGCCGGCACCATGGAATTCTCG987890.1615878275617483No Hit
AAGCGAGCGCAGGCGGTTTTTTAAGTCTGATGGAATTCTCGGGTGCCAAG920730.15060255744154563No Hit
GGGATATAGTTTAATGGTAGAACTACGGTCTGGAATTCTCGGGTGCCAAG893130.14608806287159934No Hit
AACCTGCACACACGAAACTTTGTTTAGTGGAATTCTCGGGTGCCAAGGAA890090.14559081419432987RNA PCR Primer, Index 1 (100% over 23bp)
AGCTTGTTGGCGGCAGACGCGGGGAACTGTGGAATTCTCGGGTGCCAAGG874450.14303260060469364Illumina Small RNA Adapter 2 (100% over 21bp)
TCAGTAGGTAGATGTAGACCCGAAACCAAGTGACCTGGAATTCTCGGGTG804950.13166457985790855No Hit
GCCGACTTAGCTCAGTTGGTAGAGCATCTGTGGAATTCTCGGGTGCCAAG773510.12652198169562193No Hit
ATCAGAAGGTTGTAGGTTCGACTCCTACTGGGTGCACCTGGAATTCTCGG760650.1244184889358571No Hit
GGCTCGGTAGCTCAGTCGGTAGAGCTGGAATTCTCGGGTGCCAAGGAACT753970.123325850395015RNA PCR Primer, Index 1 (100% over 25bp)
GGTTCCATGGTCTAGTTGGTTAGGACGCCTGCCTTGGAATTCTCGGGTGC753380.12322934489515021No Hit
GGATGAGGTCGTCGGTTCAAGCCCGATAGACGGCTGGAATTCTCGGGTGC750040.12268302562472917No Hit
TGTGGCGTTGCACAGACAACTAGGATGTTGGAATTCTCGGGTGCCAAGGA745470.12193551692238662RNA PCR Primer, Index 1 (100% over 22bp)
TGGCGGGAGACAGTGTCAGGTAGGCAGTGGAATTCTCGGGTGCCAAGGAA738220.12074964425455653RNA PCR Primer, Index 1 (100% over 23bp)
AGGGAGCGAGCCGTCTAAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT729090.11925626253630978RNA PCR Primer, Index 1 (100% over 30bp)
TGACCGTGGGGGTTCGAGTCCCTTCACCCGCACCTGGAATTCTCGGGTGC725340.1186428801219149No Hit
CGCGGGATGGAGCAGTTCGGTAGCTCGTCGGGCTCTGGAATTCTCGGGTG717970.11743737921682416No Hit
GCGGAAGTAGTTCAGTGGTAGAACATCACCTTGTGGAATTCTCGGGTGCC670800.1097218462869558No Hit
TCATGGAAGTTCGAGTCTTCTTGCCCGCACCATGGAATTCTCGGGTGCCA669990.1095893556854465No Hit
GGAGAGTTGGCAGAGTGGTAATGCAACGGACTGGAATTCTCGGGTGCCAA635480.10394460178657523No Hit
GCGGGCATGGCGGAATTGGTAGACGCGCAAGATTGGAATTCTCGGGTGCC627290.10260497459353682No Hit
AACGATGAATGCTAGGTGTTGGAGGGTTGGAATTCTCGGGTGCCAAGGAA620730.1015319642899554RNA PCR Primer, Index 1 (100% over 23bp)
ACCCGAAGGTCGCAGGTTCAAATCCTGCTCCCGCTGGAATTCTCGGGTGC617250.10096274540939694No Hit
ACCATTGATCTTACTCCGGGAGTCAGACAGTGGAATTCTCGGGTGCCAAG612820.1002381363171918No Hit
ACACACTGGAGGACCGAACCCATGTATGTGGAATTCTCGGGTGCCAAGGA612270.10014817356308055RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)