FastQCFastQC Report
Tue 19 Sept 2023
SRR16955387_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16955387_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61136412
Total Bases9.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCGTAGTTCTACCATTAAACTATATCCCTGATCGTCGGACTGTAGAAC44075147.209310876797938No Hit
GTCCGTTGCTCTGCCAGCTGAGCTAACCACCCGATCGTCGGACTGTAGAA22304953.648390422388543No Hit
GGCTGTGGTTTTACCACTAAACTACACCCGCGATCGTCGGACTGTAGAAC17787142.9094183675679233No Hit
GCCCGACGAGCTACCGAACTGCTCCATCCCGCGGATCGTCGGACTGTAGA16438432.688811701936319No Hit
GCGCGTCTACCAATTCCGCCATGCCCGCGATCGTCGGACTGTAGAACTCT13264062.169584305994274Illumina DpnII expression Sequencing Primer (100% over 22bp)
TCGCTCCCCATCACAGCTTGTCCTGATCGTCGGACTGTAGAACTCTGAAC12552422.0531823162929483Illumina DpnII expression Sequencing Primer (100% over 26bp)
CAGACACTCTATCCAGTTGCGCTATGGGTGCGATCGTCGGACTGTAGAAC11552481.8896234865729444No Hit
CCCTCCAACACCTAGCATTCATCGTGATCGTCGGACTGTAGAACTCTGAA10420591.7044817742984326Illumina DpnII expression Sequencing Primer (100% over 25bp)
AAGGTGATGTTCTACCACTGAACTACTTCCGCGATCGTCGGACTGTAGAA9946551.6269436943731668No Hit
GCTGGAGTTTTACCACTAAACTACGACCGCGATCGTCGGACTGTAGAACT9349401.5292686787049263No Hit
CCCTCCAACACCTAGCATTCATCGTTGATCGTCGGACTGTAGAACTCTGA8907641.4570105946027712Illumina DpnII expression Sequencing Primer (100% over 24bp)
GTCCAGTCCTACAACCCCGAGAAGCAGATCGTCGGACTGTAGAACTCTGA8803631.4399978199571148Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGGTGATGTTCTACCACTGAACTACTTCCGCGATCGTCGGACTGTAGAAC8029161.3133188123634079No Hit
TCAGACACTCTATCCAGTTGCGCTATGGGTGCGATCGTCGGACTGTAGAA7879671.2888669357959706No Hit
GGCAGGCGTCCTAACCAACTAGACCATGGAACCGATCGTCGGACTGTAGA7125971.165585248934792No Hit
GCCGTCTATCGGGCTTGAACCGACGACCTCATCCTGATCGTCGGACTGTA6469971.058284218576648No Hit
CAGACACTCTATCCAATTGCGCTATGGGTGCGATCGTCGGACTGTAGAAC6234651.0197932453085405No Hit
TCAGACACTCTATCCAATTGCGCTATGGGTGCGATCGTCGGACTGTAGAA6231891.019341795851546No Hit
GGATGCGCTCTACCGACTGAGCTAAGACGGCGATCGTCGGACTGTAGAAC5828240.9533173127660811No Hit
ACAGATGCTCTACCAACTGAGCTAAGTCGGCGATCGTCGGACTGTAGAAC5196980.850062970656505No Hit
GCATTGCTCTACCGACTGAGCTACCGAGCCGATCGTCGGACTGTAGAACT5078690.8307144357768329No Hit
GTCAGACACTCTATCCAATTGCGCTATGGGTGCGATCGTCGGACTGTAGA4159710.680398123462005No Hit
GCAGACCGCGATCACTCAGGATCTACATGTGGATCGTCGGACTGTAGAAC4153930.6794526967006176No Hit
GACAGATGCTCTACCAACTGAGCTAAGTCGGCGATCGTCGGACTGTAGAA3583120.5860860791110869No Hit
GGTGATGTTCTACCACTGAACTACTTCCGCGATCGTCGGACTGTAGAACT3454810.5650985864201518No Hit
GTCAGGTGCTCTACCAACTGAGCTAAACCGGAGATCGTCGGACTGTAGAA3212500.5254642683316123No Hit
GTCAGACACTCTATCCAGTTGCGCTATGGGTGCGATCGTCGGACTGTAGA3138640.5133830882976907No Hit
GGTCCTCCCGGATTCCGACGGAATTTGATCGTCGGACTGTAGAACTCTGA3131640.5122381077908202Illumina DpnII expression Sequencing Primer (100% over 24bp)
CCACATGCTCCACCGCTTGTGCGGGCCCCCGTGATCGTCGGACTGTAGAA2630600.430283674481911No Hit
CGCTCCCCATCACAGCTTGTCCTGATCGTCGGACTGTAGAACTCTGAACG2513600.41114614315279085Illumina Small RNA Adapter 1 (100% over 26bp)
GGTGCGGGCAAGAAGACTCGAACTTCCATGAGATCGTCGGACTGTAGAAC2508570.41032339287428254No Hit
GGTGCGGGCAAGAAGACTCGAACTTCCATGAGCAGATCGTCGGACTGTAG2394070.3915947831547589No Hit
ACTGGTACACCAGCGGAACGTCCATCCCGGTGATCGTCGGACTGTAGAAC2284570.37368401665442846No Hit
CAGGCAGGCGTCCTAACCAACTAGACCATGGAACCGATCGTCGGACTGTA2093140.3423720711643987No Hit
TGCAGACCGCGATCACTCAGGATCTACATGTGGATCGTCGGACTGTAGAA2021570.3306654633248677No Hit
CTAAACAAAGTTTCGTGTGTGCAGGTGATCGTCGGACTGTAGAACTCTGA1943460.317889116554632Illumina DpnII expression Sequencing Primer (100% over 24bp)
GGCAAGGTGATGTTCTACCACTGAACTACTTCCGCGATCGTCGGACTGTA1905570.3116915006395861No Hit
CATGGGTTCGGTCCTCCAGTGTGTGATCGTCGGACTGTAGAACTCTGAAC1733070.2834759095774217Illumina DpnII expression Sequencing Primer (100% over 26bp)
TGCATTGCTCTACCGACTGAGCTACCGAGCCGATCGTCGGACTGTAGAAC1691000.27659457673113036No Hit
CACCCTAATCATTTGTCCCACCTTAGACGGCTGATCGTCGGACTGTAGAA1573080.2573065622496787No Hit
TCCCGACTAACCCTGGGAGGACGAGCCTTGATCGTCGGACTGTAGAACTC1563300.2557068609129368Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCTGTGGTTTTACCACTAAACTACACCCGCGATCGTCGGACTGTAGAACT1535110.2510958608431257No Hit
TCAGTTCCCCGCGTCTGCCGCCAACAAGCTGATCGTCGGACTGTAGAACT1484720.24285363688009692No Hit
TGGTGCCGACTAAAGGATTCGAACCTTCGACCCCCTGTTGATCGTCGGAC1480510.24216501288953626No Hit
TCCCGACTAACCCTGGGAGGACGAGCCTGATCGTCGGACTGTAGAACTCT1462470.23921423455468732Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCATTCTACCACTGAACTACTGGGCAGATCGTCGGACTGTAGAACTCTGA1334170.21822837755019053Illumina DpnII expression Sequencing Primer (100% over 24bp)
GCAGACCGCGATCACTCAGGATCTACATGTGATCGTCGGACTGTAGAACT1271540.20798407338657687No Hit
GGTGCGGGTGAAGGGACTCGAACCCCCACGGTGATCGTCGGACTGTAGAA1268640.20750972431944487No Hit
CCAGTCCTACAACCCCGAGAAGCAGATCGTCGGACTGTAGAACTCTGAAC1248320.2041860094766438Illumina DpnII expression Sequencing Primer (100% over 26bp)
TGGATGCGCTCTACCGACTGAGCTAAGACGGCGATCGTCGGACTGTAGAA1248280.20417946673089027No Hit
GGTGCCGAGGACCGGGATCGAACCGGTACGGTCAGATCGTCGGACTGTAG1148620.187878215685932No Hit
AGCTGGAGTTTTACCACTAAACTACGACCGCGATCGTCGGACTGTAGAAC1123960.18384461292887128No Hit
GGTGCCGACTAAAGGATTCGAACCTTCGACCCCCTGTTGATCGTCGGACT1116640.18264729045597247No Hit
CGCGTCTACCAATTCCGCCATGCCCGCGATCGTCGGACTGTAGAACTCTG1074620.17577413604187303Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCCGTTGCTCTGCCAGCTGAGCTAACCACCCGATCGTCGGACTGTAGAAC1066290.17441160923869722No Hit
CCGACTTTCGTCCCTGCTCGACTGATCGTCGGACTGTAGAACTCTGAACG1050570.1718403101575539Illumina Small RNA Adapter 1 (100% over 26bp)
CCCTTCCGTTCATAGCTACCCAGCGAGATCGTCGGACTGTAGAACTCTGA1045400.17099466026890817Illumina DpnII expression Sequencing Primer (100% over 24bp)
TCAGACTTAAAAAACCGCCTGCGCTCGCTGATCGTCGGACTGTAGAACTC1037720.16973845308422747Illumina DpnII expression Sequencing Primer (100% over 21bp)
CCCTCCAACACCTAGCATTCATCGGATCGTCGGACTGTAGAACTCTGAAC1015590.1661186789960785Illumina DpnII expression Sequencing Primer (100% over 26bp)
CGCTCACTGTCTGACTCCCGGAGTGATCGTCGGACTGTAGAACTCTGAAC1009440.16511273183647088Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCAGGCGTCCTAACCAACTAGACCATGGAACCGATCGTCGGACTGTAGAA1006300.16459912629481757No Hit
GTCCAGTCCTACAACCCCGAGAAGCAAGATCGTCGGACTGTAGAACTCTG956350.15642887253507778Illumina DpnII expression Sequencing Primer (100% over 23bp)
CGGGTGCACCCACAGTTTCGGTATTATGCTTGATCGTCGGACTGTAGAAC933370.15267006509966596No Hit
TGGTGCCGGCTAGGTGACTCGAACACCTGACCCTCGGTTGATCGTCGGAC916300.14987794834934048No Hit
ACCCTCCAACACCTAGCATTCATCGTGATCGTCGGACTGTAGAACTCTGA900710.14732791319189617Illumina DpnII expression Sequencing Primer (100% over 24bp)
TCAGACTTAAAAAACCGCCTGCGCTCGCTTGATCGTCGGACTGTAGAACT873380.1428575821557863No Hit
GACCGTAGTTCTACCATTAAACTATATCCCTATCGTCGGACTGTAGAACT860740.14079007449766598No Hit
CAGTTCCCCGCGTCTGCCGCCAACAAGCTGATCGTCGGACTGTAGAACTC819460.13403796088000716Illumina DpnII expression Sequencing Primer (100% over 21bp)
CTAAACAAAGTTTCGTGTGTGCAGGTTGATCGTCGGACTGTAGAACTCTG807040.13200643832353132Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGTCACTTGGTTTCGGGTCTACATCTACCTACTGAGATCGTCGGACTGTA755750.12361700258104777No Hit
CAGATGCTCTACCAACTGAGCTAAGTCGGCGATCGTCGGACTGTAGAACT716700.11722964703914912No Hit
GCTCTACCGACTGAGCTACCGAGCCGATCGTCGGACTGTAGAACTCTGAA716130.11713641291216108Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGTGCACCCAGTAGGAGTCGAACCTACAACCTTCTGATGATCGTCGGACT706040.11548600529582927No Hit
ACATCCTAGTTGTCTGTGCAACGCCACAGATCGTCGGACTGTAGAACTCT701720.11477938875444638Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGGCAGGCGTCCTAACCAACTAGACCATGGAACCGATCGTCGGACTGTAG694560.11360823726456176No Hit
GAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGGATCGTCGGACTGTA685180.11207396338535536No Hit
CTGCCTACCTGACACTGTCTCCCGCCAGATCGTCGGACTGTAGAACTCTG670770.10971693922764066Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCCTCCAACACCTAGCATTCATCGTGATGTCGGACTGTAGAACTCTGAAC669320.10947976469407462Illumina NlaIII expression Adapter 1 (96% over 25bp)
CCTTAGACGGCTCGCTCCCTGATCGTCGGACTGTAGAACTCTGAACGTGT657660.10757255430691615Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGTGCGGGTGAAGGGACTCGAACCCCCACGGTCAGATCGTCGGACTGTAG652980.10680705305375134No Hit
CAAGGTGATGTTCTACCACTGAACTACTTCCGCGATCGTCGGACTGTAGA633820.10367307783780311No Hit
TGGTGCGGGCAAGAAGACTCGAACTTCCATGAGATCGTCGGACTGTAGAA629700.10299917502518793No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)