FastQCFastQC Report
Tue 19 Sept 2023
SRR16955388_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16955388_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55170767
Total Bases8.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGATATAGTTTAATGGTAGAACTACGGTCTGGAATTCTCGGGTGCCAA49320848.93966908236023No Hit
GGGTGGTTAGCTCAGCTGGCAGAGCAACGGACTGGAATTCTCGGGTGCCA34723506.293822233792762No Hit
GCGGGTGTAGTTTAGTGGTAAAACCACAGCCTGGAATTCTCGGGTGCCAA22179224.020103617555289No Hit
CGCGGGATGGAGCAGTTCGGTAGCTCGTCGGGCTGGAATTCTCGGGTGCC19072553.4570028725538653No Hit
GCGGAAGTAGTTCAGTGGTAGAACATCACCTTTGGAATTCTCGGGTGCCA13019762.3599019386480524No Hit
GGTTCCATGGTCTAGTTGGTTAGGACGCCTGCCTGGAATTCTCGGGTGCC10196421.8481562889999334No Hit
GCGGTCGTAGTTTAGTGGTAAAACTCCAGCTGGAATTCTCGGGTGCCAAG8432531.5284416836184278No Hit
GCACCCATAGCGCAACTGGATAGAGTGTCTGATGGAATTCTCGGGTGCCA8327741.5094479291904714No Hit
GCGGGCATGGCGGAATTGGTAGACGCGCTGGAATTCTCGGGTGCCAAGGA8214381.4889008158976655RNA PCR Primer, Index 1 (100% over 22bp)
TCCGGTTTAGCTCAGTTGGTAGAGCACCTGACTGGAATTCTCGGGTGCCA7917021.435002707140178No Hit
AGGATGAGGTCGTCGGTTCAAGCCCGATAGACGGCTGGAATTCTCGGGTG7626461.3823371351716027No Hit
GCACCCATAGCGCAATTGGATAGAGTGTCTGACTGGAATTCTCGGGTGCC7165841.2988472681556158No Hit
GCACCCATAGCGCAATTGGATAGAGTGTCTGATGGAATTCTCGGGTGCCA7081011.2834713717139368No Hit
AGGACAAGCTGTGATGGGGAGCGATGGAATTCTCGGGTGCCAAGGAACTC7047541.2774047531367472RNA PCR Primer, Index 1 (100% over 26bp)
GCGGAAGTAGTTCAGTGGTAGAACATCACCTTGGAATTCTCGGGTGCCAA7006911.2700403458229972No Hit
GCACCCATAGCGCAACTGGATAGAGTGTCTGTGGAATTCTCGGGTGCCAA6962381.2619690424097239No Hit
GCCGTCTTAGCTCAGTCGGTAGAGCGCATCCTGGAATTCTCGGGTGCCAA5831991.057079739348195No Hit
GCCGACTTAGCTCAGTTGGTAGAGCATCTGTTGGAATTCTCGGGTGCCAA5828441.056436282642219No Hit
GCCGACTTAGCTCAGTTGGTAGAGCATCTGTCTGGAATTCTCGGGTGCCA5758861.0438245312050853No Hit
TGCTCATGGAAGTTCGAGTCTTCTTGCCCGCACCTGGAATTCTCGGGTGC5387040.97643014460901No Hit
GCACCCATAGCGCAACTGGATAGAGTGTCTGACTGGAATTCTCGGGTGCC5331020.9662762165332955No Hit
GCGGAAGTAGTTCAGTGGTAGAACATCACCTTGCCTGGAATTCTCGGGTG4794270.8689873751437966No Hit
TGCTTCTCGGGGTTGTAGGACTGGACTGGAATTCTCGGGTGCCAAGGAAC4743190.8597288487941449RNA PCR Primer, Index 1 (100% over 24bp)
GCACCCATAGCGCAATTGGATAGAGTGTCTGTGGAATTCTCGGGTGCCAA4079580.7394459460750292No Hit
GGTTCCATGGTCTAGTTGGTTAGGACGCCTGCCTGTGGAATTCTCGGGTG4056090.7351882564909783No Hit
CACATGTAGATCCTGAGTGATCGCGGTCTGCTGGAATTCTCGGGTGCCAA3380940.6128136663389145No Hit
GCCGTCTTAGCTCAGTCGGTAGAGCGCATCCATGGAATTCTCGGGTGCCA3075790.5575035779364822No Hit
AACGATGAATGCTAGGTGTTGGAGGGTGGAATTCTCGGGTGCCAAGGAAC2856210.5177035149792281RNA PCR Primer, Index 1 (100% over 24bp)
GGCTCGGTAGCTCAGTCGGTAGAGCAATGCTGGAATTCTCGGGTGCCAAG2777560.503447776972178No Hit
ACGATGAATGCTAGGTGTTGGAGGGTGGAATTCTCGGGTGCCAAGGAACT2515470.4559425465301217RNA PCR Primer, Index 1 (100% over 25bp)
TAAAGGAGGAAATTTTAGCATGACTGTTAAGATGGAATTCTCGGGTGCCA2489520.4512389686371408No Hit
TCATGGAAGTTCGAGTCTTCTTGCCCGCACCTGGAATTCTCGGGTGCCAA2079170.376860811088597No Hit
GGTTCCATGGTCTAGTTGGTTAGGACGCCTGCCTTGGAATTCTCGGGTGC1992070.3610734648659135No Hit
CACATGTAGATCCTGAGTGATCGCGGTCTGCATGGAATTCTCGGGTGCCA1932210.35022351601528395No Hit
AAATTCCGTCGGAATCCGGGAGGACCTGGAATTCTCGGGTGCCAAGGAAC1843200.3340899719592443RNA PCR Primer, Index 1 (100% over 24bp)
GCGGTCGTAGTTTAGTGGTAAAACTCCAGCTTGGAATTCTCGGGTGCCAA1780090.3226509430256788No Hit
GCGGAAGTAGTTCAGTGGTAGAACATCACCTTGTGGAATTCTCGGGTGCC1717330.31127535348566027No Hit
TGACCGTGGGGGTTCGAGTCCCTTCACCCGCACCTGGAATTCTCGGGTGC1684430.3053120504922471No Hit
GGCTCGGTAGCTCAGTCGGTAGAGCAATGCATGGAATTCTCGGGTGCCAA1679440.30440758599567774No Hit
GCGGAAGTAGTTCAGTGGTAGAACATCACCTGGAATTCTCGGGTGCCAAG1678650.3042643942216718No Hit
GCGGAAGTAGTTCAGTGGTAGAACATCACCTTGCTGGAATTCTCGGGTGC1660940.3010543609081962No Hit
ACCGTGGGGGTTCGAGTCCCTTCACCCGCACCTGGAATTCTCGGGTGCCA1592770.28869817959935196No Hit
AGCCGTCTAAGGTGGGACAAATGATTAGGGTGTGGAATTCTCGGGTGCCA1506490.27305946281297844No Hit
AAAGGAGGAAATTTTAGCATGACTGTTAAGATGGAATTCTCGGGTGCCAA1406270.2548940456093351No Hit
ACATGTAGATCCTGAGTGATCGCGGTCTGCTGGAATTCTCGGGTGCCAAG1375040.24923343915084595No Hit
GCGGGTGTAGTTTAGTGGTAAAACCACAGCTGGAATTCTCGGGTGCCAAG1334380.2418635941747919No Hit
TGACCGTACCGGTTCGATCCCGGTCCTCGGCACCTGGAATTCTCGGGTGC1239180.22460807913002187No Hit
TCCGGTTTAGCTCAGTTGGTAGAGCACCTGACTTGGAATTCTCGGGTGCC1222680.22161736486280859No Hit
AGCTTGTTGGCGGCAGACGCGGGGAACTGATGGAATTCTCGGGTGCCAAG1210780.21946042548221237No Hit
ACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTGGAATTCTCGGGTGCCA1185120.21480941165817036No Hit
AGGACAAGCTGTGATGGGGAGCGTGGAATTCTCGGGTGCCAAGGAACTCC1179200.21373637963017625RNA PCR Primer, Index 1 (100% over 27bp)
ACCTGCACACACGAAACTTTGTTTAGTGGAATTCTCGGGTGCCAAGGAAC1155100.2093681242459435RNA PCR Primer, Index 1 (100% over 24bp)
AAGGACAAGCTGTGATGGGGAGCGATGGAATTCTCGGGTGCCAAGGAACT1152650.20892404849111484RNA PCR Primer, Index 1 (100% over 25bp)
TGCCCAGTAGTTCAGTGGTAGAATGCTGGAATTCTCGGGTGCCAAGGAAC1093440.1981919156570725RNA PCR Primer, Index 1 (100% over 24bp)
TTGACCGTGGGGGTTCGAGTCCCTTCACCCGCACCTGGAATTCTCGGGTG1092720.19806141176177597No Hit
GGGTGGTTAGCTCAGCTGGCAGAGCAACGGATGGAATTCTCGGGTGCCAA1000490.18134422528510435No Hit
GCGGGCATGGCGGAATTGGTAGACGCGCAAGATTGGAATTCTCGGGTGCC975720.1768545287760817No Hit
GCGGGCATGGCGGAATTGGTAGACGCGCAAGATTTGGAATTCTCGGGTGC961800.1743314534670145No Hit
AACAGGGGGTCGAAGGTTCGAATCCTTTAGTCGGCACCATGGAATTCTCG914770.165807011528406No Hit
GGATGAGGTCGTCGGTTCAAGCCCGATAGACGGCTGGAATTCTCGGGTGC896150.1624320357917083No Hit
CGCGGGATGGAGCAGTTCGGTAGCTCGTCGGGCTTGGAATTCTCGGGTGC866900.15713031504528477No Hit
ACCGGGATGGACGTTCCGCTGGTGTACCAGTTGGAATTCTCGGGTGCCAA863970.15659923669359174No Hit
GGGATATAGTTTAATGGTAGAACTACGGTCTGGAATTCTCGGGTGCCAAG822910.149156889553484No Hit
GGTTCCATGGTCTAGTTGGTTAGGACGCCTGCTGGAATTCTCGGGTGCCA819060.1484590562244676No Hit
TGGGTAGTAGCGAAGTGGTAGCGCAGTGGACTGGAATTCTCGGGTGCCAA755250.13689314850380818No Hit
AACAGGGGGTCGAAGGTTCGAATCCTTTAGTCGGCACCTGGAATTCTCGG729600.13224394723386754No Hit
AACCGAGGGTCAGGTGTTCGAGTCACCTAGCCGGCACCATGGAATTCTCG722380.13093528317269904No Hit
GCCGGGGTGGCGGAATTGGCAGACGCACAGGACTGGAATTCTCGGGTGCC698510.12660871653279715No Hit
CGCGGGATGGAGCAGTTCGGTAGCTCGTCGGGCTCTGGAATTCTCGGGTG695030.12597794770553036No Hit
TTGCTTCTCGGGGTTGTAGGACTGGACTGGAATTCTCGGGTGCCAAGGAA671820.121771009636317RNA PCR Primer, Index 1 (100% over 23bp)
GGAGAGTTGGCAGAGTGGTAATGCAACGGACTGGAATTCTCGGGTGCCAA657680.11920805813702028No Hit
ATCCGTGGGTCCAGGGTTCGATCCCCTGACCACCCATGGAATTCTCGGGT629810.11415646985658183No Hit
ATCAGAAGGTTGTAGGTTCGACTCCTACTGGGTGCACCTGGAATTCTCGG628770.11396796423004234No Hit
AACCTGCACACACGAAACTTTGTTTAGTGGAATTCTCGGGTGCCAAGGAA613540.1112074443336994RNA PCR Primer, Index 1 (100% over 23bp)
TGCCCAGTAGTTCAGTGGTAGAATGCTTGACTGGAATTCTCGGGTGCCAA613420.1111856936844833No Hit
AAGCATAATACCGAAACTGTGGGTGCACCCGTGGAATTCTCGGGTGCCAA613160.11113856727784843No Hit
ATCCACGCGAGGGTTCGAATCCCTCATTCTCCTGGAATTCTCGGGTGCCA601120.10895625213983341No Hit
AGGATGAGGTCGTCGGTTCAAGCCCGATAGACGGCTCTGGAATTCTCGGG592820.10745183223572007No Hit
GGCTCGGTAGCTCAGTCGGTAGAGCAATGCATTTGGAATTCTCGGGTGCC586470.10630086038136827No Hit
GCACCCATAGCGCAATTGGATAGAGTGTCTGACTGCGTGGAATTCTCGGG570860.10347146342917436No Hit
GCGGGTGTGGCGGAACTGGCAGACGCGCTAGATTTGGAATTCTCGGGTGC569570.10323764395010132No Hit
TGGGTAGTAGCGAAGTGGTAGCGCAGTGGATGGAATTCTCGGGTGCCAAG560710.10163172101631285No Hit
GAGCCGTTAGCTCAGTTGGTAGAGCAACTGACTGGAATTCTCGGGTGCCA556720.10090851192987764No Hit
ACACACTGGAGGACCGAACCCATGTGGAATTCTCGGGTGCCAAGGAACTC555370.10066381712619656RNA PCR Primer, Index 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)