FastQCFastQC Report
Tue 19 Sept 2023
SRR16955389_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16955389_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55170767
Total Bases8.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCGTAGTTCTACCATTAAACTATATCCCTGATCGTCGGACTGTAGAAC47056848.529306833816538No Hit
GTCCGTTGCTCTGCCAGCTGAGCTAACCACCCGATCGTCGGACTGTAGAA32938995.970370141854291No Hit
GGCTGTGGTTTTACCACTAAACTACACCCGCGATCGTCGGACTGTAGAAC20897353.7877577449666417No Hit
GCCCGACGAGCTACCGAACTGCTCCATCCCGCGGATCGTCGGACTGTAGA18328113.322069095033607No Hit
AAGGTGATGTTCTACCACTGAACTACTTCCGCGATCGTCGGACTGTAGAA12357822.2399217324638605No Hit
GGCAGGCGTCCTAACCAACTAGACCATGGAACCGATCGTCGGACTGTAGA9469691.716432544793151No Hit
GCTGGAGTTTTACCACTAAACTACGACCGCGATCGTCGGACTGTAGAACT8024051.4544024736868348No Hit
GCGCGTCTACCAATTCCGCCATGCCCGCGATCGTCGGACTGTAGAACTCT7783791.4108540488480068Illumina DpnII expression Sequencing Primer (100% over 22bp)
TCAGACACTCTATCCAGTTGCGCTATGGGTGCGATCGTCGGACTGTAGAA7697831.3952733337928762No Hit
GTCAGGTGCTCTACCAACTGAGCTAAACCGGAGATCGTCGGACTGTAGAA7472131.354363987725601No Hit
GCCGTCTATCGGGCTTGAACCGACGACCTCATCCTGATCGTCGGACTGTA7335861.3296643129866221No Hit
AGGTGATGTTCTACCACTGAACTACTTCCGCGATCGTCGGACTGTAGAAC6672301.209390473038013No Hit
TCGCTCCCCATCACAGCTTGTCCTGATCGTCGGACTGTAGAACTCTGAAC6668961.208785079968165Illumina DpnII expression Sequencing Primer (100% over 26bp)
GTCAGACACTCTATCCAATTGCGCTATGGGTGCGATCGTCGGACTGTAGA6593641.1951329224768616No Hit
TCAGACACTCTATCCAATTGCGCTATGGGTGCGATCGTCGGACTGTAGAA6482391.1749682580994387No Hit
CAGACACTCTATCCAGTTGCGCTATGGGTGCGATCGTCGGACTGTAGAAC6475821.1737774100548575No Hit
GGATGCGCTCTACCGACTGAGCTAAGACGGCGATCGTCGGACTGTAGAAC5576551.0107798573835307No Hit
ACAGATGCTCTACCAACTGAGCTAAGTCGGCGATCGTCGGACTGTAGAAC5458700.9894189072992224No Hit
GACAGATGCTCTACCAACTGAGCTAAGTCGGCGATCGTCGGACTGTAGAA5394400.9777641844275974No Hit
GGTGCGGGCAAGAAGACTCGAACTTCCATGAGCAGATCGTCGGACTGTAG5090980.9227676678846971No Hit
GTCAGACACTCTATCCAGTTGCGCTATGGGTGCGATCGTCGGACTGTAGA4942560.8958657399125882No Hit
GGCAAGGTGATGTTCTACCACTGAACTACTTCCGCGATCGTCGGACTGTA4597450.8333126853211956No Hit
GTCCAGTCCTACAACCCCGAGAAGCAGATCGTCGGACTGTAGAACTCTGA4482280.8124374997360468Illumina DpnII expression Sequencing Primer (100% over 24bp)
CAGGCAGGCGTCCTAACCAACTAGACCATGGAACCGATCGTCGGACTGTA3846580.6972134355137749No Hit
CAGACACTCTATCCAATTGCGCTATGGGTGCGATCGTCGGACTGTAGAAC3752690.6801953650562806No Hit
GCAGACCGCGATCACTCAGGATCTACATGTGGATCGTCGGACTGTAGAAC3223210.5842242504984569No Hit
TGGATGCGCTCTACCGACTGAGCTAAGACGGCGATCGTCGGACTGTAGAA2936690.5322909503868235No Hit
GCATTGCTCTACCGACTGAGCTACCGAGCCGATCGTCGGACTGTAGAACT2671740.48426732947178347No Hit
CCCTCCAACACCTAGCATTCATCGTTGATCGTCGGACTGTAGAACTCTGA2598420.4709776828007484Illumina DpnII expression Sequencing Primer (100% over 24bp)
TCTTAACAGTCATGCTAAAATTTCCTCCTTTAGATCGTCGGACTGTAGAA2350650.4260680298318129No Hit
CCCTCCAACACCTAGCATTCATCGTGATCGTCGGACTGTAGAACTCTGAA2261550.40991817278886117Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGTGCGGGCAAGAAGACTCGAACTTCCATGAGATCGTCGGACTGTAGAAC1978680.35864645492421743No Hit
AGGCAGGCGTCCTAACCAACTAGACCATGGAACCGATCGTCGGACTGTAG1858630.33688674293761406No Hit
TGCAGACCGCGATCACTCAGGATCTACATGTGGATCGTCGGACTGTAGAA1836240.33282843430471065No Hit
GGTCCTCCCGGATTCCGACGGAATTTGATCGTCGGACTGTAGAACTCTGA1762870.31952972486316894Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGCTGGAGTTTTACCACTAAACTACGACCGCGATCGTCGGACTGTAGAAC1694750.3071826063248314No Hit
CAAGGTGATGTTCTACCACTGAACTACTTCCGCGATCGTCGGACTGTAGA1639320.2971356189410961No Hit
TGCATTGCTCTACCGACTGAGCTACCGAGCCGATCGTCGGACTGTAGAAC1613440.2924447289268246No Hit
GGTGATGTTCTACCACTGAACTACTTCCGCGATCGTCGGACTGTAGAACT1600670.29013009733941164No Hit
GCAAGGTGATGTTCTACCACTGAACTACTTCCGCGATCGTCGGACTGTAG1581370.2866318679238228No Hit
GGTGCGGGTGAAGGGACTCGAACCCCCACGGTCAGATCGTCGGACTGTAG1532230.2777249770698312No Hit
GGTGCGGGTGAAGGGACTCGAACCCCCACGGTGATCGTCGGACTGTAGAA1441580.26129417414117156No Hit
CACCCTAATCATTTGTCCCACCTTAGACGGCTGATCGTCGGACTGTAGAA1424560.2582092070606885No Hit
GCAGACCGCGATCACTCAGGATCTACATGTGATCGTCGGACTGTAGAACT1301100.23583141412552772No Hit
TCTTAACAGTCATGCTAAAATTTCCTCCTTTGATCGTCGGACTGTAGAAC1294790.23468769248758134No Hit
GCTGTGGTTTTACCACTAAACTACACCCGCGATCGTCGGACTGTAGAACT1256180.22768942110230223No Hit
AGTCAGGTGCTCTACCAACTGAGCTAAACCGGAGATCGTCGGACTGTAGA1150990.2086231645102922No Hit
TCAGTTCCCCGCGTCTGCCGCCAACAAGCTGATCGTCGGACTGTAGAACT1150680.2085669753331506No Hit
GGTGCCGAGGACCGGGATCGAACCGGTACGGTCAGATCGTCGGACTGTAG1149560.20836396927380035No Hit
CGCTCCCCATCACAGCTTGTCCTGATCGTCGGACTGTAGAACTCTGAACG1137150.20611458963403573Illumina Small RNA Adapter 1 (100% over 26bp)
TCGCTCCCCATCACAGCTTGTCCTTGATCGTCGGACTGTAGAACTCTGAA1082020.19612197887334065Illumina DpnII expression Sequencing Primer (100% over 25bp)
CTAAACAAAGTTTCGTGTGTGCAGGTGATCGTCGGACTGTAGAACTCTGA1079010.1955764000888369Illumina DpnII expression Sequencing Primer (100% over 24bp)
CCACATGCTCCACCGCTTGTGCGGGCCCCCGTGATCGTCGGACTGTAGAA1069630.1938762243417787No Hit
GCATTCTACCACTGAACTACTGGGCAGATCGTCGGACTGTAGAACTCTGA1028750.1864665031754951Illumina DpnII expression Sequencing Primer (100% over 24bp)
GGTGCGGGTGAAGGGACTCGAACCCCCACGGTCAAGATCGTCGGACTGTA1023210.18546234820335197No Hit
TCCGTTGCTCTGCCAGCTGAGCTAACCACCCGATCGTCGGACTGTAGAAC949670.17213282534208743No Hit
ATCTTGCGCGTCTACCAATTCCGCCATGCCCGCGATCGTCGGACTGTAGA930080.16858203185755966No Hit
AATCTTGCGCGTCTACCAATTCCGCCATGCCCGCGATCGTCGGACTGTAG915600.1659574535188173No Hit
TGGTGCCGACTAAAGGATTCGAACCTTCGACCCCCTGTTGATCGTCGGAC878700.15926912888486758No Hit
AGCCCGACGAGCTACCGAACTGCTCCATCCCGCGGATCGTCGGACTGTAG830970.15061780815916515No Hit
GACCGTAGTTCTACCATTAAACTATATCCCTATCGTCGGACTGTAGAACT801860.1453414631701604No Hit
GCAGGCGTCCTAACCAACTAGACCATGGAACCGATCGTCGGACTGTAGAA771910.13991286363664293No Hit
ACTGGTACACCAGCGGAACGTCCATCCCGGTGATCGTCGGACTGTAGAAC740410.13420331821741757No Hit
GCCGTCTATCGGGCTTGAACCGACGACCTCATCCGATCGTCGGACTGTAG723540.13114553944845467No Hit
GTCCACTGCGCTACCACTTCGCTACTACCCAGATCGTCGGACTGTAGAAC717690.13008519529916995No Hit
GGTGCCGACTAAAGGATTCGAACCTTCGACCCCCTGTTGATCGTCGGACT702320.12729929964540823No Hit
TGGTGCCGGCTAGGTGACTCGAACACCTGACCCTCGGTTGATCGTCGGAC674030.12217158409271345No Hit
GAGCCCGACGAGCTACCGAACTGCTCCATCCCGCGGATCGTCGGACTGTA668940.12124899405513068No Hit
GTCCTGTGCGTCTGCCAATTCCGCCACCCCGGCGATCGTCGGACTGTAGA653790.11850297459159848No Hit
GTCCAGTCCTACAACCCCGAGAAGCAAGATCGTCGGACTGTAGAACTCTG636820.11542707028162215Illumina DpnII expression Sequencing Primer (100% over 23bp)
GTCCGTTGCATTACCACTCTGCCAACTCTCCGATCGTCGGACTGTAGAAC632090.11456973219168769No Hit
TGGGTGGTCAGGGGATCGAACCCTGGACCCACGGATGATCGTCGGACTGT603950.10946920495051302No Hit
GGTGCACCCAGTAGGAGTCGAACCTACAACCTTCTGATGATCGTCGGACT586090.1062319833255173No Hit
GTCAAGCATTCTACCACTGAACTACTGGGCAGATCGTCGGACTGTAGAAC583130.10569546731152024No Hit
CGGGTGCACCCACAGTTTCGGTATTATGCTTGATCGTCGGACTGTAGAAC568710.10308176429738597No Hit
GAGCCGTCTATCGGGCTTGAACCGACGACCTCATCCTGATCGTCGGACTG565950.10258149936541576No Hit
AATGCATTGCTCTACCGACTGAGCTACCGAGCCGATCGTCGGACTGTAGA563460.10213017339418173No Hit
CTAAACAAAGTTTCGTGTGTGCAGGTTGATCGTCGGACTGTAGAACTCTG561290.10173684915419066Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGAGAATGAGGGATTCGAACCCTCGCGTGGATGATCGTCGGACTGTAGAA561120.10170603573446786No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)