FastQCFastQC Report
Thu 24 Aug 2023
SRR17007092_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR17007092_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128701992
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length26
%GC17

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNN119867329.313555923827503No Hit
ATGCTGAGTTTTTTTTTTTTTTTTTT25783292.0033326290707296No Hit
TTCGGTGATTTTTTTTTTTTTTTTTT21997681.709194990548398No Hit
AGTCTTCGTTTTTTTTTTTTTTTTTT21727291.6881859917133215No Hit
TGAGACACTTTTTTTTTTTTTTTTTT21500091.6705328072933012No Hit
AAGTGGTGTTTTTTTTTTTTTTTTTT21364581.6600038327301103No Hit
CATCCTAGTTTTTTTTTTTTTTTTTT18445941.4332287879429246No Hit
CTAGCTGTTTTTTTTTTTTTTTTTTT17526511.361790111220656No Hit
GATGATCCTTTTTTTTTTTTTTTTTT17388751.351086314188517No Hit
GCTTAGACTTTTTTTTTTTTTTTTTT16159741.2555936197164688No Hit
CCCAGTTATTTTTTTTTTTTTTTTTT15877181.23363902557157No Hit
TCCTACCATTTTTTTTTTTTTTTTTT15541951.20759203167578No Hit
GCAATTCCTTTTTTTTTTTTTTTTTT15415511.1977677859096385No Hit
TAGAGGGTTTTTTTTTTTTTTTTTTT15376751.194756177511223No Hit
AGAAGCCATTTTTTTTTTTTTTTTTT15280071.1872442502677039No Hit
TGGAATTCTCGGGTGCCAAGGAACTC14704061.1424889212281968RNA PCR Primer, Index 1 (100% over 26bp)
CGGCATAATTTTTTTTTTTTTTTTTT14366491.1162601119647004No Hit
TCGTCTAGTTTTTTTTTTTTTTTTTT14191061.102629398307992No Hit
CCTCTATCTTTTTTTTTTTTTTTTTT13621231.0583542483165296No Hit
AGGGAACATTTTTTTTTTTTTTTTTT13364991.0384446885639502No Hit
ATGCGCTTTTTTTTTTTTTTTTTTTT12987181.0090892765669082No Hit
TCGGGATATTTTTTTTTTTTTTTTTT12903371.0025773338457729No Hit
AGCAGGATTTTTTTTTTTTTTTTTTT12870631.000033472675388No Hit
CAGAATGGTTTTTTTTTTTTTTTTTT12576070.9771464920294318No Hit
TAGCCATCTTTTTTTTTTTTTTTTTT12570830.97673934992397No Hit
GTAGTGTCTTTTTTTTTTTTTTTTTT12465170.9685296867821596No Hit
GCCTAATGTTTTTTTTTTTTTTTTTT11889770.9238217540564562No Hit
TGTAGTGGTTTTTTTTTTTTTTTTTT11610510.9021235661993483No Hit
TCTCGGATTTTTTTTTTTTTTTTTTT11602380.901491874344882No Hit
AGTGAGAGTTTTTTTTTTTTTTTTTT11415660.8869839403884284No Hit
AAGCAGGATTTTTTTTTTTTTTTTTT11091110.8617667704785796No Hit
TTGGTACCTTTTTTTTTTTTTTTTTT11041450.8579082443417038No Hit
CAAGGGATTTTTTTTTTTTTTTTTTT10930650.8492992089819401No Hit
GCTCATGATTTTTTTTTTTTTTTTTT10843910.8425596085567968No Hit
CTCTCTCATTTTTTTTTTTTTTTTTT10651630.8276196688548535No Hit
GAGTCGATTTTTTTTTTTTTTTTTTT9959880.7738714720126476No Hit
TACCTTGGTTTTTTTTTTTTTTTTTT9862910.7663370120953528No Hit
GGCTCAAATTTTTTTTTTTTTTTTTT9452620.7344579406354488No Hit
CATTAGCGTTTTTTTTTTTTTTTTTT9054040.7034887230028266No Hit
GCAACGTTTTTTTTTTTTTTTTTTTT8961250.6962790443834No Hit
GACCAGTTTTTTTTTTTTTTTTTTTT8959900.696174150902031No Hit
TAGCGTCATTTTTTTTTTTTTTTTTT8843840.6871564194593041No Hit
TCAACAGGTTTTTTTTTTTTTTTTTT8785330.6826102582778982No Hit
TAAGGACGTTTTTTTTTTTTTTTTTT8690950.6752770384470818No Hit
GACTGTGTTTTTTTTTTTTTTTTTTT8676000.6741154402645143No Hit
CTGCCAATTTTTTTTTTTTTTTTTTT8660880.672940633273182No Hit
GTCCTAGTTTTTTTTTTTTTTTTTTT8659540.6728365167805639No Hit
ACTCCACTTTTTTTTTTTTTTTTTTT8395610.6523294526785568No Hit
TTACTCGCTTTTTTTTTTTTTTTTTT8380480.6511538686984736No Hit
ATCTGACGTTTTTTTTTTTTTTTTTT8365200.6499666298871272No Hit
TCGATGCATTTTTTTTTTTTTTTTTT8161000.6341005195941334No Hit
GTTTCAGGTTTTTTTTTTTTTTTTTT8151470.6333600493145436No Hit
CTGAAGCTTTTTTTTTTTTTTTTTTT8100080.6293671041237652No Hit
TCCGCTTTTTTTTTTTTTTTTTTTTT8078280.6276732686468443No Hit
ACCGTCATTTTTTTTTTTTTTTTTTT8065820.6267051406632463No Hit
GATATCGCTTTTTTTTTTTTTTTTTT7981610.6201621183920759No Hit
CCTTTGGTTTTTTTTTTTTTTTTTTT7930320.6161769430888062No Hit
GTTAGCCTTTTTTTTTTTTTTTTTTT7887340.612837445437519No Hit
CGCCTTTTTTTTTTTTTTTTTTTTTT7808260.6066930183955506No Hit
ACAGATCGTTTTTTTTTTTTTTTTTT7745660.6018290688150343No Hit
ACTGAAGCTTTTTTTTTTTTTTTTTT7713070.5992968624759126No Hit
CGTGTCTATTTTTTTTTTTTTTTTTT7574900.5885612089049873No Hit
GCAGGTAATTTTTTTTTTTTTTTTTT7550620.5866746802178477No Hit
CCACACTTTTTTTTTTTTTTTTTTTT7337520.5701170499365698No Hit
AGCTCTTGTTTTTTTTTTTTTTTTTT7313680.568264708754469No Hit
CTCTGGTTTTTTTTTTTTTTTTTTTT7254180.563641625686726No Hit
TGTCACTGTTTTTTTTTTTTTTTTTT7229520.5617255714270529No Hit
ACCAAGTCTTTTTTTTTTTTTTTTTT6853850.5325364350226995No Hit
GGTACTCATTTTTTTTTTTTTTTTTT6732010.5230696040819632No Hit
ACGTGTGATTTTTTTTTTTTTTTTTT6619700.5143432434208167No Hit
CTTACACCTTTTTTTTTTTTTTTTTT6614650.5139508641016217No Hit
GTTCCCAATTTTTTTTTTTTTTTTTT6535840.507827415755927No Hit
GGATGATCTTTTTTTTTTTTTTTTTT6496940.5048049295149992No Hit
TAACAGCCTTTTTTTTTTTTTTTTTT6380090.4957258159609526No Hit
GAGGACTATTTTTTTTTTTTTTTTTT6348740.4932899562269401No Hit
GTGTTGGATTTTTTTTTTTTTTTTTT6320210.4910732073206761No Hit
TACACCAGTTTTTTTTTTTTTTTTTT6120200.47553265531430156No Hit
AGGTTAGGTTTTTTTTTTTTTTTTTT5972590.46406352436254444No Hit
TGGGTTGTTTTTTTTTTTTTTTTTTT5883680.4571553173784599No Hit
TGGAAAGCTTTTTTTTTTTTTTTTTT5811900.45157809212463473No Hit
CCCATACTTTTTTTTTTTTTTTTTTT5630770.4375044948799239No Hit
TTATCCCGTTTTTTTTTTTTTTTTTT5559450.4319630111086392No Hit
GATAGGAGTTTTTTTTTTTTTTTTTT5410190.4203656770129867No Hit
AAGGCTCTTTTTTTTTTTTTTTTTTT5399490.41953429904954387No Hit
GCACTAAGTTTTTTTTTTTTTTTTTT5117860.39765196485847704No Hit
CTGTGTACTTTTTTTTTTTTTTTTTT4898160.3805815220016175No Hit
CACTTAGCTTTTTTTTTTTTTTTTTT4869560.37835933417409734No Hit
CCTTGCAATTTTTTTTTTTTTTTTTT4665560.3625087636561212No Hit
CAATCCGATTTTTTTTTTTTTTTTTT4611300.3582928226938399No Hit
GTACGACATTTTTTTTTTTTTTTTTT4195630.32599573128596177No Hit
ACCCCTAATTTTTTTTTTTTTTTTTT4166760.3237525647621678No Hit
GTTGGAACTTTTTTTTTTTTTTTTTT3622770.2814851537029823No Hit
TGCACGTATTTTTTTTTTTTTTTTTT3593620.2792202314941637No Hit
GTCGATAGTTTTTTTTTTTTTTTTTT3552990.27606332619933344No Hit
GTAAACGGTTTTTTTTTTTTTTTTTT3278890.2547660645376802No Hit
AGGACTACTTTTTTTTTTTTTTTTTT3145960.24443755307221662No Hit
GGGTATCTTTTTTTTTTTTTTTTTTT2442030.18974298393143751No Hit
GCAGGTGGAATTCTCGGGTGCCAAGG1962990.15252211480922534Illumina Small RNA Adapter 2 (100% over 21bp)
TGTAGTGGAATTCTCGGGTGCCAAGG1904460.14797439965031778Illumina Small RNA Adapter 2 (100% over 21bp)
GTTGGAATTCTCGGGTGCCAAGGAAC1660800.12904229174634685RNA PCR Primer, Index 1 (100% over 24bp)
TTGGAATTCTCGGGTGCCAAGGAACT1505210.11695312377138654RNA PCR Primer, Index 1 (100% over 25bp)
CTTGAGGATTTTTTTTTTTTTTTTTT1354750.10526255102562825No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGG1335800.10379015734270841No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)