FastQCFastQC Report
Tue 18 Jul 2023
SRR17007093_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR17007093_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82230089
Total Bases6.2 Gbp
Sequences flagged as poor quality0
Sequence length39-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA14420791.753712074907276No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA13488111.6402888728479912No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATACG11591751.4096725591528911No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT7387910.898443634178725No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCAA7182350.873445485386742No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT4915570.5977824005517007No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCA4131860.5024754381574366No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAATA3865190.4700457031002362No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA3644120.44316138342012495No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT3152060.383321973541826No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATACGAC3040380.36974057026741153No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA3011880.3662746856567309No Hit
TCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT2826620.34374521958744325No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATACGA2711720.32977223215701496No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA2584560.31430830629406226No Hit
TCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA2470730.30046544154901744No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATCTGTAGGCACCATCAAT2389430.2905785496596021No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATA2271720.2762638381675593No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATACG2220020.26997660187379824No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAAT2209350.26867902331955396No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAC2207600.2684662058434596No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA2041830.24830691840793218No Hit
ACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATACGAC1997550.24292202821281147No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA1937600.23563150953174816No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1849980.22497604252866613No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATAC1840570.22383169255720978No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAC1814270.22063334991647643No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT1791830.2179044218230142No Hit
TGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGCTGTAGGCACCATC1790920.21779375673544513No Hit
CTGTAGGCACCATCAATACGACTATCTCGTATGCCGTCTTCTGCTTGAAA1744620.21216321436791827Illumina PCR Primer Index 1 (96% over 27bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAC1683820.20476932719846624No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCAA1570090.19093862345205537No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATACGAC1544550.18783270440094016No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATCTGTAGGCACCATCAA1348420.16398133778014032No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATACGA1326680.16133753667711584No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATACGA1318800.16037924998475922No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA1286630.156467056724212No Hit
TTGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTCTGTAGGCACCATCAA1284000.15614722246013865No Hit
TTGGCGCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATA1272720.15477546181422713No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATACG1208140.14692188889641114No Hit
TAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATC1182700.1438281308439299No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAC1152460.14015064485701823No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATACGACT1108020.1347462970640832No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAC1057370.12858675125597882No Hit
TTGTGAAGCAGAATTCACCAAGCGTTGGATTGTCTGTAGGCACCATCAAT1041180.12661788557713954No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT1024490.1245882149051304No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA1006140.12235667165579743No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATACGAC974060.11845542329402074No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAA972630.11828152101355503No Hit
ACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATACG970360.11800546634456396No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATACGAC928390.11290149521788793No Hit
TACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATAC923620.11232141558304772No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAC906070.11018716032278647No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA893390.10864514569599944No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA841500.10233480350483387No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)