FastQCFastQC Report
Tue 25 Jul 2023
SRR17007094_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR17007094_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58642149
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length43-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA16603642.8313491717365267No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT5895491.0053332117825355No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATC3620810.6174415606767754No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATATCA3230200.5508324737553529No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT3087950.5265751771818594No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCAA2960290.5048058521866243No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCA2861300.4879255021844442No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT2810980.4793446433895183No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCAG2672610.4557489869615795No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA2528050.43109777576534586No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA2380780.40598443962208824No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATATCA2319660.39556190207149466No Hit
TGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGCTGTAGGCACCATC2253760.3843242511457075No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2236510.38138268091096045No Hit
TTGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTCTGTAGGCACCATCAA2210170.3768910310568598No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2188800.37324689448198767No Hit
TCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1693580.28879910250219515No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAATATCAGT1660950.2832348452987287No Hit
ACGCAGTTTTATCCGGTAAAGCGAATGATTCTGTAGGCACCATCAATATC1549680.26426043834103013No Hit
TCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT1538270.2623147388408293No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC1508780.2572859326829922No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATCAGTATCTCGT1485120.2532512920015943No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATAT1474740.25148123408642475No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCAG1427730.24346481572494896No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGCCTGTAGGCACCATCAATA1425560.24309477471570834No Hit
TTGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATC1384730.23613220586442013No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA1380470.23540576591079568No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT1301990.22202290028627703No Hit
TTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGCTGTAGGCACCAT1282660.21872663636525327No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCAATATCA1269930.21655584279491533No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT1256010.21418212350983248No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATATCA1179840.20119317250805388No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAATATC1160530.19790031910324435No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAATA1120700.19110827606266612No Hit
TCTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATATCA1115140.19016015255511867No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCA1088920.1856889657983032No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCAGT1047020.17854393432955534No Hit
TTGTCAAAGTGAAGAAATTCAATGAAGCGCCTGTAGGCACCATCAATATC1030290.1756910375163775No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT1017700.17354411755953897No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA1015270.17312973983951374No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCCTGTAGGCACCATCAA1004090.17122326127577625No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT1000920.17068269445582562No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAT993340.16939010881064404No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCATCAATATC982790.16759106150765382No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATAT962790.16418054529345438No Hit
TTGTCAAAGTGAAGAAATTCAATGAAGCGCTGTAGGCACCATCAATATCA962540.1641379138407769No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAAT962200.1640799350651355No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTCTGTAGGCACCATCA936550.15970594802042468No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATATC927880.15822748924156924No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAT925330.1577926484242588No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACCATCAATA925320.1577909431661517No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA897600.15306396769327127No Hit
ACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATATCAG869280.14823467673396484No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAAT861680.14693868057256906No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATATCAG844930.14408237324317702No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATA843150.1437788373001133No Hit
ACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATATCAGTATCT837770.14286140843849363No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT817230.1393588082865108No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATATCAG815390.13904504079480443No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATCTGTAGGCACCATCAAT806770.13757510830648445No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA794580.1354963986739299No Hit
TCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCAT772700.13176529393559572No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCTGTAGGCACCATCAATAT757560.12918353316144673No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTCTGTAGGCACCATC735100.12535352345290074No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATCTGTAGGCACCATCAATATCA732220.12486240911805603No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATCTGTAGGCACCATCAA728920.12429967394271312No Hit
CTGTAGGCACCATCAATATCAGTATCTCGTATGCCGTCTTCTGCTTGAAA721940.1231094037839575RNA PCR Primer, Index 40 (96% over 29bp)
TGTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCAATAT719670.12272231019364588No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA714130.12177759720231263No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA703840.120022886610107No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA700880.11951813021040548No Hit
TGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGGCTGTAGGCACCAT700130.11939023585237302No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA696130.11870813260953313No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATATC691050.11784186149112645No Hit
TTGTCAAAGTGAAGAAATTCAATGAAGCGCGGCTGTAGGCACCATCAATA690160.11769009351959459No Hit
TATTCGTAGACGACCTGCTTCTGGGTCGGCTGTAGGCACCATCAATATCA661760.11284716049543136No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA631300.1076529443012056No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCATC630350.10749094478103113No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT621480.10597838084003368No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA614280.10475059500292187No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATA604100.10301464224989436No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATATCA592440.10102631129701607No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA589330.10049597602570805No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)