FastQCFastQC Report
Tue 18 Jul 2023
SRR17007095_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR17007095_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51584710
Total Bases3.9 Gbp
Sequences flagged as poor quality0
Sequence length36-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATC9371571.8167340671295815No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATCAG5211771.0103323252180734No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATCAG2791870.5412204507886155No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT2755030.5340787997063471No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT2718240.5269468414187072No Hit
CTGTAGGCACCATCAATCAGCATATCTCGTATGCCGTCTTCTGCTTGAAA2175700.421772265463933RNA PCR Primer, Index 10 (96% over 29bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATC1578130.30592979974104734No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATC1556020.30164364595633086No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATCAGCA1368480.2652879118638062No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAAT1347230.2611684741466997No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATCAGCATA1275040.24717401726209182No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT1271090.24640828648644142No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATCAGC1256200.24352177224607835No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATC1233600.23914062907400274No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAATC1218140.2361436169748749No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA1212640.23507740956574147No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATCAGCA1188280.23035508002274319No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATCA1175260.22783107630148544No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATCAGCATATCTCGT1157570.22440176556192715No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATC1138160.220639022687149No Hit
TCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1128700.21880514594343944No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATCA1060110.20550857027208255No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT1043610.20230994804468225No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCA1005650.1949511783627358No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATCAGC927300.17976256917989847No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATCAG926880.17968114970501917No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA847250.16424440497969262No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATC846290.1640583033228257No Hit
CTAAACCATTCGTAGACGACCTGCTTCCTGTAGGCACCATCAATCAGCAT826140.1601521070875459No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATCA818700.1587098192468272No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCAA813090.1576222876895111No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT812410.1574904656825637No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT805020.1560578706364735No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATCAGC714280.13846738694469737No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAATC689650.13369271631070526No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATCAGCAT671640.13020137168552465No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATCA656770.12731873456301296No Hit
TCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT654100.12680113932985182No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATCTGTAGGCACCATCAA653990.12677981518166914No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATCA634980.12309461466391883No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATCAGCAT620690.12032441395909757No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATCAGC606610.11759492299171596No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATCAGCATA595150.11537333446286702No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATCTGTAGGCACCATCAAT587590.11390778391503995No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATCA576240.11170751953437366No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATC557380.10805139740050879No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA540390.1047577857857493No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATCAGCA529660.10267771205847624No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)