FastQCFastQC Report
Tue 18 Jul 2023
SRR17007096_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR17007096_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38671033
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length55-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATGCAGCTA7494711.9380682176242874No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATGCA5365601.3874984927348593No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATGCAGCTA4745831.2272312456716634No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATGCAGCT3849140.9953548435078008No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATGCAGC3782830.9782076418801638No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATG3727920.9640083832257597No Hit
TATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATGCAGCTA3278640.8478283991017256No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATGCAG3070470.7939974088615631No Hit
CTGTAGGCACCATCAATGCAGCTATCTCGTATGCCGTCTTCTGCTTGAAA2411260.6235313134769377RNA PCR Primer, Index 38 (96% over 29bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT2113330.5464891511949008No Hit
TAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATGCAGCTAT1979900.5119852888336343No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATG1975130.5107518074316763No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATGCAGC1800710.465648279941216No Hit
CTAAACCATTCGTAGACGACCTGCTTCCTGTAGGCACCATCAATGCAGCT1710030.442199203729572No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATGC1550040.40082715142365094No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATGCAGC1457750.37696174291490997No Hit
ACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATGCAGCTATCT1347870.34854771011676877No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATGCAG1326070.34291041565918345No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATG1318630.3409864949819158No Hit
AGCTCTCGCTGGCCCTTGAAAATCCGGCTGTAGGCACCATCAATGCAGCT1312970.33952286715485464No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATGCAGCT1221810.3159496670285482No Hit
TCTAAACCATTCGTAGACGACCTGCTTCCTGTAGGCACCATCAATGCAGC1196210.30932972491321864No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATGCAGCTAT1182530.3057921933453394No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATGCAGCT1176350.3041940979440606No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATGCAGC1159220.29976442573954515No Hit
TGAGCTCTCGCTGGCCCTTGAAAATCCGGCTGTAGGCACCATCAATGCAG1142250.2953761281732505No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATGCAGC1051870.2720046294082705No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATGC992710.25670635692612603No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATGCAG990400.2561090105868131No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT979920.2533989717833501No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATGC908550.2349432972219801No Hit
AAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATGCAGCTAT873770.2259494852387315No Hit
ACTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATGCAGCT853720.2207647258866863No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATGCA852230.22037942456825502No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT846140.2188046024009754No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATGCAGCTATCTCGT832170.21519207930132098No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATGCAGCT800630.2070361037420438No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATG794230.20538111821321142No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA775910.2006437221369287No Hit
ATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATGCAGCTATCTCGTA772810.19984208852140048No Hit
TCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGCTGTAGGCACCATCAA759700.19645195410218289No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATGCAGC752450.19457716580780243No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATG742040.191885228408561No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATGCA738110.19086896385726237No Hit
TATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATGCAGCT729490.18863990522311622No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATGCAGCT710190.18364908948773104No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATGCAG681740.17629216162909328No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATGC674800.17449753669626566No Hit
TTGGCGCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATG663110.17147460219125774No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATG644330.16661825403009017No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT640440.16561233313834672No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATGCAGCT636600.1646193418210473No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATGCAG634960.16419525177928398No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATGCA622210.16089821029606322No Hit
TATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATGCAGC617780.15975265000032454No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATGC595540.15400157528763195No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATGCAGCT594640.1537688429476399No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATGCAGC594310.15368350775630948No Hit
TCGCTGGCCCTTGAAAATCCGGCTGTAGGCACCATCAATGCAGCTATCTC581630.15040456767731028No Hit
ACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATGCAGC573450.1482892892982714No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATGC572850.14813413440494336No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATGCAGCT567390.14672222487565822No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATG564550.14598782504723884No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATGC557480.1441595832208568No Hit
TATTCGTAGACGACCTGCTTCTGGGTCGGCTGTAGGCACCATCAATGCAG531540.137451719999308No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATGCAGCT505710.13077230184153601No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAATGCAG501520.12968880350312856No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATGCA482850.1248609004057378No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATGCAG476570.12323694585557102No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATGC462700.11965028190480456No Hit
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATGCAGCTA437970.11325531438480063No Hit
TTGAGCTCTCGCTGGCCCTTGAAAATCCGGCTGTAGGCACCATCAATGCA434600.11238386106727483No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATGCAGC424560.10978760251891902No Hit
ACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATGCAGCTATCTC423440.10949798005137334No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATGCA414150.10709566511967757No Hit
GCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATGCAGCTA396940.10264530559605169No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATGC395870.10236861270295004No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATGCAGCT392230.10142733968342661No Hit
TAGCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATGCAGCTA391360.10120236508810096No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)