FastQCFastQC Report
Tue 18 Jul 2023
SRR17007097_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR17007097_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44284145
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length52-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATTACGATA4448081.004440754134465No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATTACGAT3641750.8223597858782189No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT3138410.7086983388750082No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATTACGATA3109060.7020706846660356No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATTACG2946320.6653216405103903No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATTACGATA2852880.6442215379793378No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATTACGA2782440.6283151678778037No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTAC2681120.6054356474535073No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT2388370.539328466203875No Hit
ACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATTACGATATCT1987350.4487723540784179No Hit
TCTAAACCATTCGTAGACGACCTGCTTCCTGTAGGCACCATCAATTACGA1911370.43161497190473025No Hit
CTAAACCATTCGTAGACGACCTGCTTCCTGTAGGCACCATCAATTACGAT1892900.42744417894937337No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTACGA1878810.4242624533001597No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT1596740.3605669704134516No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATTAC1484900.3353118819387842No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATTACGATAT1375890.310695848367401No Hit
TAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATTACGATAT1270720.286946942297294No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATTACGA1140530.2575481586016846No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT1083740.24472415578984308No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA1044810.23593319911674937No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT1026940.23189789483346693No Hit
TCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGCTGTAGGCACCATCAA968700.21874646106411225No Hit
TGAGCTCTCGCTGGCCCTTGAAAATCCGGCTGTAGGCACCATCAATTACG952420.21507020176182698No Hit
CTGTAGGCACCATCAATTACGATATCTCGTATGCCGTCTTCTGCTTGAAA916570.20697475360538178RNA PCR Primer, Index 31 (100% over 29bp)
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATTACGAT891810.2013835877377784No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATTA842370.19021932115884818No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTACGA839740.1896254291462554No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATTACGAT818940.184928488514343No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATTACG809590.182817123374517No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATT774770.1749542641051329No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATT769810.17383422441598456No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATTA748090.1689295344868914No Hit
TCCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATTACGA705240.159253385156245No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTACGA695580.1570720175358472No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATTACGAT689080.15560422358837459No Hit
ACTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATTACGAT684600.15459257483688577No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATT670330.1513702025860497No Hit
TATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATTACGAT667470.15072437324916174No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTAC648360.14640905904359222No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATTACGAT621550.1403549735464013No Hit
TTGGCGCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATT617170.1393659062402582No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT616000.13910170332971317No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATT605020.1366222606307517No Hit
AAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATTACGATAT601400.1358048123092362No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATTACG597240.13486542418285372No Hit
TATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATTACGA596870.13478187283507448No Hit
AGCTCTCGCTGGCCCTTGAAAATCCGGCTGTAGGCACCATCAATTACGAT586790.13250566314422463No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATTACGA565350.12766420126209954No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATTACGAT563420.1272283793669269No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAATTAC544670.12299435836460204No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGCTGTAGGCACCATCAATTACG542590.1225246643014108No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA521050.11766062097393999No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATTACGAT520140.11745512982129383No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTACG515820.11647961138235817No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATTACGA515560.11642089962445928No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATTA510630.11530763436891464No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTACGATATCTCGT505650.11418307839069716No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTA505490.114146948078144No Hit
TTGAGCTCTCGCTGGCCCTTGAAAATCCGGCTGTAGGCACCATCAATTAC494200.11159750289861077No Hit
TCATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATTACGAT483390.10915644865673707No Hit
TAGCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATTACGATA481380.10870256160528785No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTA473670.10696153216913186No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATTA462280.10438950554425293No Hit
CATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATTACGATA461150.10413433521184612No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTACG457000.10319720522999824No Hit
ACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACCATCAATTACGATA453620.10243395237731247No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATTACG451980.10206361667364244No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)