FastQCFastQC Report
Sat 2 Sept 2023
SRR17096848.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR17096848.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182044679
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length1-101
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGTCGGTAGAGCATCAGA21862721.200953530753843No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAC12754450.7006219610516603No Hit
GGGGGGGATGCGTGCATTTATCAGA11937730.655758249325156No Hit
GTCTAGGGGTATGATTCTCGC10523470.5780707273514982No Hit
CTCAGTCGGTAGAGCATGAGA10126360.5562568516490394No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC9822100.5395433722069954No Hit
GTCTAGGGGTATGATTCTCGCTT9813690.5390813977045712No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA9259340.5086300819591656No Hit
A8260230.4537474012080298No Hit
GATGCGTGCATTTATCAGA7982450.43848850973556885No Hit
GTGGTTCAGTGGTAGAATTCTCGC7528230.4135374920790736No Hit
ATTGATCATCGACACTTCGAACGCAC7525450.4133847823148953No Hit
GTCTAGGGGTATGATTCTCGCT7133510.39185490282855234No Hit
ATGCGTGCATTTATCAGA7060300.3878333625999582No Hit
CTACAGTCCGACGATC6869770.37736725059676146Illumina DpnII expression Sequencing Primer (100% over 16bp)
ACAGTCCGACGATC6858020.37672180465104393Illumina DpnII expression Sequencing Primer (100% over 14bp)
CTCAGTCGGTAGAGCATCAGAC6657570.36571077147495207No Hit
TACAGTCCGACGATC6208220.3410272705636181Illumina DpnII expression Sequencing Primer (100% over 15bp)
AGATCGGAAGAGCACACG6150780.33787200119153166Illumina Multiplexing PCR Primer 2.01 (100% over 18bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC5905850.32441761178858736No Hit
GTCTAGGGGTATGATTCTCG5811940.31925898806413344No Hit
AGTCCGACGATC5506960.3025059578917987Illumina DpnII expression Sequencing Primer (100% over 12bp)
GGGGGGATGCGTGCATTTATCAGA5428330.29818668855462677No Hit
CTCAGTCGGTAGAGCATGAGAC5362180.29455296520916163No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAC5341680.29342686802727147No Hit
TTCCCGGGCGGCGCA5309510.29165971942525165No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT5083200.2792281558528827No Hit
AGAGCACTGG4758560.26139517101733034No Hit
ATGCGTGCATTTATCAGAT4708370.2586381555266441No Hit
GGCGGGTGTTGACGCGATGTGA4694240.2578619724446876No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGT4628630.2542579121469406TruSeq Adapter, Index 1 (100% over 50bp)
ACGGCCCTGGCGGAGCGCTGAGAAGACG4431860.24344902714789046No Hit
GGCGGGTGTTGACGCGATGTGAT4348890.23889135479757692No Hit
AGATCGGAAGAGCAC4325390.23760046290614184Illumina Multiplexing PCR Primer 2.01 (100% over 15bp)
GTGGTTCAGTGGTAGAATTCT4273500.23475006374671353No Hit
GTGGTTCAGTGGTAGAATTCTCG4215600.23156952585249693No Hit
AGATCGGAAGAGCACA4167170.22890918992474368Illumina Multiplexing PCR Primer 2.01 (100% over 16bp)
GTCTAGGGGTATGATTCT4114100.22599397151289438No Hit
ACCATCTGTGGGATTATGACTGAAC4054390.2227140074772523No Hit
TCTGACT3997870.21960927514942638No Hit
TCTACAGTCCGACGATC3858430.21194961704977933Illumina DpnII expression Sequencing Primer (100% over 17bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCG3832310.21051480444534168TruSeq Adapter, Index 1 (100% over 50bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTC3712840.20395212979556518TruSeq Adapter, Index 1 (100% over 50bp)
GCACCACCGGCCCGTCTCGC3676490.20195536723157945No Hit
ATCCCGGACGAG3656000.20082981936538777No Hit
GCGCAGCGGAAGCGTGCTGGGC3513690.19301250766027608No Hit
GTATAGTGGTGAGTATCCCCGC3488870.19164910609664124No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG3362840.1847260803486599No Hit
ATGCGTGCATTTATCAGATC3327360.1827771082504422No Hit
CCATCTGTGGGATTATGACTGAAC3292910.18088471566916822No Hit
AGATCGGAAGAGCA3277510.1800387694935044Illumina Multiplexing PCR Primer 2.01 (100% over 14bp)
GTGGTTCAGTGGTAGAATTCTCGCC3269340.17958997856784378No Hit
TCCGACGATC3243130.1781502221221198Illumina DpnII expression Sequencing Primer (100% over 10bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGC3237890.1778623806961147No Hit
GTCTAGGGGTATGATTCTCGG3136630.17230000993327577No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGC3127640.17180617512033955No Hit
TTCTACAGTCCGACGATC3094210.16996981274031087Illumina DpnII expression Sequencing Primer (100% over 18bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAC3020030.1658949888889639No Hit
GCTACGCCTGTCTGAGCGTCGCT2998990.16473922865935564No Hit
AGTTCTACAGTCCGACGATC2955110.1623288313744122No Hit
AGATCGGAAGAG2788520.15317778115338376Illumina Single End Adapter 2 (100% over 12bp)
ATTGATCATCGACACTTCGAACGC2740480.1505388685378714No Hit
CTAGCTCAGTCGGTAGAGCATGAGAC2720660.14945012482347808No Hit
TTCCCGGGCGGCG2636460.14482488664225115No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT2597880.1427056266775037No Hit
ATAGCTCAGTCGGTAGAGCATCAGAC2578760.1416553350620042No Hit
GGGGGGGGATGCGTGCATTTATCAGA2539990.13952563809898558No Hit
GCATTGGTGGTTCAGTGGTAGAATTCT2520070.13843140122760741No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC2493600.13697736257372292No Hit
GTATAGTGGTGAGTATC2492090.13689441590325196No Hit
GGCGGGTGTTGACGCGATGGG2472380.13581171466154196No Hit
TCGACTCCCGGTGTGGGAAC2453910.13479712856644385No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG2443530.13422693887141848No Hit
GTGGTATAGTGGTGAGCATAGC2419560.1329102291421547No Hit
AGATCGGAAGAGCACAC2401940.13194233488142765Illumina Multiplexing PCR Primer 2.01 (100% over 17bp)
TGTGGGATTATGACTGAAC2357760.12951545812552973No Hit
GGCGGGTGTTGACGCGATGTGATT2350100.12909468230049173No Hit
GGCGGGTGTTGACGCGATGTG2329350.12795485222613948No Hit
TACCATCTGTGGGATTATGACTGAAC2319760.1274280584712943No Hit
GTTCTACAGTCCGACGATC2280510.1252719943547485Illumina DpnII expression Sequencing Primer (100% over 19bp)
GGGGGATGCGTGCATTTATCAGA2227220.12234469099753253No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA2213550.12159377643770626No Hit
AGGCGTCCAGTGCGGTAACGCGACCGA2212660.12154488734054127No Hit
ATTGATCATCGACACTTCGAACGCA2207780.12127682127968156No Hit
GGGGATGCGTGCATTTATCAGA2193960.12051766698437806No Hit
CATCTGTGGGATTATGACTGAAC2189360.12026498176307587No Hit
GGATGCGTGCATTTATCAGA2185590.12005788974474778No Hit
GTGGTTCAGTGGTAGAATTCTC2168000.11909164343111617No Hit
TAGGATTCGGCG2131740.11709982470841677No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAG2108130.11580289034429839No Hit
CACCACCGGCCCGTCTCGC2085990.11458670538785701No Hit
CAGTCCGACGATC2070000.11370834958598268Illumina DpnII expression Sequencing Primer (100% over 13bp)
GTATAGTGGTGAGTATCCCCGCC2067680.11358090834393461No Hit
ATCCCGGACGA2030980.11156491973050198No Hit
AGGCGTCCAGTGCGGTAACGCGACCG2009060.11036081971942723No Hit
GTGGTCTAGTGGTTAGGATTCG1985240.1090523497256407No Hit
TAGGATTCGGCGC1975050.10849259702888651No Hit
TTGATCATCGACACTTCGAACGCAC1971650.10830582969140229No Hit
CCCCGTTCCTCCCGACCCC1957410.10752360413676249No Hit
GGGGGGGATGCGTGCATTTATCAGAT1919990.10546806479303907No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC1900540.10439964575948962No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGA1896700.10418870853127216No Hit
CTAGCTCAGTCGGTAGAGCATGAGA1894250.10405412618514381No Hit
ATCGTATAGTGGTTAGTACTCTGC1858300.1020793362490974No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCC1844170.1013031531671409No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG1838120.10097081716955868No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)