FastQCFastQC Report
Sat 2 Sept 2023
SRR17096851.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR17096851.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285199721
Total Bases7.3 Gbp
Sequences flagged as poor quality0
Sequence length1-101
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGC83606202.9314965564079216No Hit
CTCAGTCGGTAGAGCATGAGA33766881.1839731077436784No Hit
GTGGTTCAGTGGTAGAATTCTCGC32410001.1363966236138077No Hit
GTCTAGGGGTATGATTCTCGC31156321.092438656347774No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC26223950.9194942375136474No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCG20046690.7029000564835756No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC18937280.6640006495658528No Hit
GTATAGTGGTGAGTATCCCCGC17763180.6228330076101302No Hit
CTCAGTCGGTAGAGCATCAGA17529000.6146219196336451No Hit
TCGACTCCCGGTGTGGGAAC17145450.6011734492545314No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC15727430.5514532042617251No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT12146910.425908901923505No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA12136680.42555020592043286No Hit
GTCTAGGGGTATGATTCTCG11899160.41722200702994383No Hit
TTCGACTCCCGGTGTGGGAAC11813360.414213588939661No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGA10716600.37575773084294145No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA9553380.33497157593642946No Hit
GTGGTTCAGTGGTAGAATTCT8804680.30871979709966124No Hit
GTCTAGGGGTATGATTCTCGG8702450.30513529148929286No Hit
CTCAGTCGGTAGAGCATGG8493450.2978070935770656No Hit
TGTGGTTCAGTGGTAGAATTCTCGC8377180.2937303013701055No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG8150290.2857748237418507No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA8021830.28127061176192386No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGC7980650.27982671133117976No Hit
GTGGTTCAGTGGTAGAATTCTCG7861830.2756605080970609No Hit
CTAGCTCAGTCGGTAGAGCATGAGA7584970.2659529249679736No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT7487860.2625479426748808No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCG7472440.26200726893417964No Hit
GCACCACCGGCCCGTCTCGC7266770.2547958313044773No Hit
GGGGGGGCCGGGCCACCCCTCCCACGGCGC7170520.2514210033185832No Hit
CTCAGTCGGTAGAGCATGAGAC7083030.2483533285083403No Hit
GTATAGTGGTGAGTATCCCCG7006070.24565486864554117No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGC6970670.24441363320969028No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAG6897260.2418396475219553No Hit
CTCAGTCGGTAGAGCATGA6891710.2416450470510804No Hit
CTCAGTCGGTAGAGCATGAG6882900.24133614071803386No Hit
GTGGTTCAGTGGTAGAATTCTCGCC6797700.23834876051649434No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGC6789280.23805352881113093No Hit
AGACCGGGGTTCGATTCCCCGACGGGGAGC6774160.23752337401480136No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC6607620.2316839573626371No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGC6401660.22446235142004226No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGC6243970.21893324362684072No Hit
CTCAGTCGGTAGAGCATGGGAC6211340.2177891331106877No Hit
GTCTAGGGGTATGATTCTCGCT6074080.21297636542919338No Hit
CTCAGTCGGTAGAGCATCAGAC6051440.2121825357606153No Hit
GCATTGGTGGTTCAGTGGTAGAATTCT5853360.2052372274235149No Hit
TTCCCGGGCGGCGCA5791490.2030678704626082No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAC5788960.20297916069840755No Hit
GTGGTTCAGTGGTAGAATTCTC5632100.19747915531796753No Hit
GTCTAGGGGTATGATTCTCGCTT5628660.19735853808917295No Hit
GGCCACCCCTCCCACGGCGC5594160.19614885948643687No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGC5579210.19562466542525125No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGG5514190.19334485954844255No Hit
GTATAGTGGTGAGTATCCCC5489400.19247564411186785No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGC5438170.19067935904467453No Hit
TCGACTCCCGGTGTGGGAA5330250.18689534412272443No Hit
GGGCCACCCCTCCCACGGCGC5276080.18499597340068927No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG5224160.18317549476144124No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG5210110.18268285753337044No Hit
TGGTGGTTCAGTGGTAGAATTCTCGC5190540.18199667172886191No Hit
CGCAGCGGAAGCGTGCTGGGC4956280.17378277870054437No Hit
CTCAGTCGGTAGAGCATGGGA4865000.17058221455974004No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG4598640.1612427944836594No Hit
AGAGCACTGG4411530.1546821288790812No Hit
CCGCCACGCAGTTTTATCCGGTAAAGC4307700.15104152223206416No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAC4284070.1502129800470597No Hit
ATAGCTCAGTCGGTAGAGCATCAGA4271300.14976522364830785No Hit
GGGGGGGATGCGTGCATTTATCAGA4271010.1497550553354153No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGT4256370.14924173085008033No Hit
GTATAGTGGTGAGTATCCCCGCC4233650.14844509612966977No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGG4001940.14032061412851102No Hit
GTATAGTGGTGAGTATCCC3943690.13827818576302184No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC3937490.1380607942460084No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG3917130.13734690855465456No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT3899470.13672769336264531No Hit
TTCGACTCCCGGTGTGGGAA3879630.1360320405082023No Hit
ATTGATCATCGACACTTCGAACGCAC3841960.13471121172660613No Hit
GTTCAGTGGTAGAATTCTCGC3722230.13051310102789337No Hit
CCACCCCTCCCACGGCGC3624910.12710075547374045No Hit
CACCACCGGCCCGTCTCGC3618920.12689072721778716No Hit
GGGAGACCGGGGTTCGATTCCCCGACGG3602590.12631814601249208No Hit
GTGGTCTAGTGGTTAGGATTCG3532790.1238707382886956No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCG3521710.12348223860990384No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCG3506850.1229612002320297TruSeq Adapter, Index 4 (100% over 50bp)
CGCGACCTCAGATCAGACGTGGCGACCCGC3497870.12264633316383924No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGA3435900.120473469888142No Hit
GAGGGCGCACCACCGGCCCGTCTCGC3385720.11871400112624934No Hit
GGGTTCGATTCCCCGACGGG3316120.11627360603203396No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAC3312610.11615053438288601No Hit
TCTGACT3283620.11513405372510865No Hit
GTGGTCTAGTGGTTAGGATTC3249010.11392051817610298No Hit
GCGCAGCGGAAGCGTGCTGGGC3229810.11324730573631941No Hit
AGACCGGGGTTCGATTCCCCGACGGGGAG3227380.11316210228690932No Hit
ACGCCTGTCTGAGCGTCGCT3227030.11314983018514244No Hit
ATTGATCATCGACACTTCGAACGC3205690.11240158260884132No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG3194950.1120250044003374No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGC3190300.11186196076257732No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGA3144440.11025396479963598No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGG3140030.11009933631737319No Hit
ATCCCGGACGAG3058110.10722696324096334No Hit
TCAGTGGTAGAATTCTCGC3054390.10709652833075527No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAC2996470.10506567080407488No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGC2921060.10242155882052913No Hit
GTCTAGCGGTTAGGATTCCTGG2883250.1010958211982262No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)