FastQCFastQC Report
Tue 19 Sept 2023
SRR17238154_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR17238154_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34136356
Total Bases981.6 Mbp
Sequences flagged as poor quality0
Sequence length5-38
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC367151510.755439157009025No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC23338786.836927761123654No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC10451923.061814799447252No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCG6504301.9053879095940998No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG5986451.7536874761910732No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCG5752591.6851798709856436No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCG5046561.4783534598713466No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC4889241.4322676972316553No Hit
GAGGGCGCACCACCGGCCCGTCTCGC4665081.3666016372690746No Hit
AGCTATTAATGGTTCGTTTGT2379460.6970456952112873No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGCGC2280310.6680004157444339No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCGT2276260.6668139973698423No Hit
AGAGCCACCTTACAAATAAGCGCTCTC2156650.6317751080402372No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCG1744250.5109654937978735No Hit
GGCCCGAGGTGGGATCCCGAGGCCT1649450.48319451554817394No Hit
GAGGGCGCACCACCGGCCCGTCTCG1638270.479919414948684No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGC1580950.46312793316310624No Hit
CGAAGCCGAGCGCACGGGGTCGGCGGCGA1559970.4569819930399132No Hit
CGAGGGCGCACCACCGGCCCGTCTCGC1520150.4453170104038053No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC1473190.43156041611471363No Hit
CGAGGGCGCACCACCGGCCCGTCTCG1416120.4148421700312711No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGA1389700.40710262102961425No Hit
AGGTCGGTTTCTATCTATTTACGATTTCTC1262740.36991060205723186No Hit
GGCCCGAGGTGGGATCCCGAGGCCTCT1241260.36361819052976835No Hit
TTGGTGACTCTAGATAACCTCG1105760.32392443997244463No Hit
GGTTGGCCCCGGATAGCCGGGTCCCCG1087940.31870419912424164No Hit
ACCTCTAGCATTACAAGTATTAGAGGC1056190.3094032649530606No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGC1005000.2944075225838399No Hit
AGGGCGCACCACCGGCCCGTCTCGC940930.2756386768406095No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCCGT934630.2737931371468003No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA919630.2693989950186833No Hit
GTCGGTTTCTATCTATTTACGATTTCTC916550.2684967311683766No Hit
TTAAAGCATCTGGCCTACACCCAGAAGATT875510.25647435830584847No Hit
TAACCTAGACTTACAAGTCAAAGTAA778580.22807941187395633No Hit
GGCCCGAGGTGGGATCCCGAGGCCTC755140.22121283244175213No Hit
TAACCTAGACTTACAAGTCAAAGTA745150.2182863337844262No Hit
TAGAGCCACCTTACAAATAAGCGCTCTCA724250.21216382908591647No Hit
GGGAGAGCGACGCTCAGAC723100.21182694485609418No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC692660.20290976576410208No Hit
CGGTTGGCCCCGGATAGCCGGGTCCC686550.20111988520391572No Hit
CACCTCTAGCATTACAAGTATTAGAGGC667100.19542214757779067No Hit
GGGAGAGCGACGCTCAGACA646630.18942560828695365No Hit
AAGCTATTAATGGTTCGTTTGT629390.1843752742677045No Hit
TAGAGCCACCTTACAAATAAGCGCTCTC603310.1767353258209517No Hit
TTGGTGACTCTAGATAACCTC593520.173867415725334No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGA586220.17172893322298372No Hit
GTTGGCCCCGGATAGCCGGGTCCCCG576190.16879071685331615No Hit
TGAGTCTTAAACCAATTTTTTGAGGCCTT572770.16778885244810549No Hit
TCCGCCCGGAGGATTCAACCCGGCGGCGCGC553470.16213505624326158No Hit
GAAGCCGAGCGCACGGGGTCGGCGGCGA552850.16195343170196608No Hit
GGGAGAGCGACGCTCAGACAG552530.16185969000323291No Hit
AGAGCCACCTTACAAATAAGCGCTCTCA539880.15815396347518756No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCG529660.15516008797189718No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGCG519410.15215742418435055No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAG517710.1516594214098306No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC515060.1508831229671966No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCCG514800.1508069578369759No Hit
TCGACTGCATAATTTGTGGTAGTGGGG514210.15063412157993664No Hit
CCGAGGGCGCACCACCGGCCCGTCTCGC511870.1499486354079504No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGC498300.1459734014960472No Hit
ATTCAACCCGGCGGCGCGC490530.1436972358736826No Hit
CACCTCTAGCATTACAAGTATTAGAGGCA460650.1349441047544735No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCG456860.1338338515101026No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGAG436040.12773478223627618No Hit
ATTAAAGCATCTGGCCTACACCCAGAAGATT435440.1275590165511515No Hit
CCTCTAGCATTACAAGTATTAGAGGC427990.12537659262752007No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGA410750.1203262586082709No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCG410160.12015342235123162No Hit
CGAAGCCGAGCGCACGGGGTCGGCGGCG405390.11875608515449043No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAATCG397210.11635981298062394No Hit
CTCGACTGCATAATTTGTGGTAGTGGGG395310.11580322164439578No Hit
GGGAGAGCGACGCTCAGA395180.11576513907928544No Hit
TCGGTTTCTATCTATTTACGATTTCTC386850.11332492548413778No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCC386680.1132751252066858No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCG378260.11080854675876944No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACG373430.10939363299351577No Hit
TCGCTTCTGGCGCCAAGCGTCCG367930.10778244754653954No Hit
AGTCCGCCCGGAGGATTCAACCCGGC359100.1051957625471213No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCC356620.1044692643819393No Hit
GGGAGAGCGACCCCC355600.10417046271722734No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC343900.10074303185729606No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGC343340.10057898388451306No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)