FastQCFastQC Report
Tue 19 Sept 2023
SRR17238156_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR17238156_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83862681
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length5-38
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC18825742.2448292584397582No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGG14129781.6848710095495278No Hit
GGGAGAGATCC13246651.5795643356548545No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACC6944780.8281132819972689No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGC5592740.6668925836034266No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCG5544750.6611701335901723No Hit
GGGAGAAAGCCC5367960.6400892430329052No Hit
GCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCC4990530.5950835270816109Illumina Paired End PCR Primer 2 (100% over 38bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGC4666890.5564918679382549No Hit
GGGAGAAAGCC4142070.4939109924234356No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC3358400.4004641826320816No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGA3353950.399933553280988No Hit
GGGAGAGCGACGCTCAGAC3306960.3943303458185411No Hit
GGGAGAGCGACGCTCAGACAGGCGTAGCCCCGGGAGGA3239360.38626954938395064No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC3064540.36542356665177445No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCG2934830.34995661538652695No Hit
GGGAGAAAGCCCC2921670.34838738341789954No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCG2759770.3290820144421569No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC2725840.3250361146932567No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCG2589660.30879766412428433No Hit
TCGCTTCTGGCGCCAAGCGTCCG2522220.30075594649782306No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCCG2317490.27634341907099297No Hit
GGGAGAGCGACGCTCAGACA2314100.2759391868237554No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGA2191920.2613701319660887No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGA2149380.25629755385473546No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG2137090.2548320629053107No Hit
GGGAGAGCGACCCC2048360.2442516713721566No Hit
GGGAGAGCGACCCCC2040110.2432679203279943No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCG2020570.2409379208852147No Hit
GGGAGAGATCCTTCCGCAGGTTCACCTACGGAAACCTT1974990.2355028454194065No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAC1945570.23199472957464834No Hit
AGGTCGGTTTCTATCTATTTACGATTTCTC1872900.22332937340746356No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTT1870640.2230598852426385No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCC1848370.22040435363615435No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCG1843760.2198546454769315No Hit
CCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTC1769520.21100207850497887No Hit
AGCTATTAATGGTTCGTTTGT1766040.2105871144281686No Hit
AAGCTATTAATGGTTCGTTTGT1748140.20845267276871343No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC1723320.20549307265766997No Hit
GGGAGAGCGACGCTCAGA1678830.200187971572242No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC1672040.19937831465225872No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCG1655550.19741200498944222No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG1580160.18842230908406088No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGC1575700.1878904873074592No Hit
CTCGCTTCTGGCGCCAAGCGTCCG1573090.1875792642498515No Hit
GGGGAGAGATCC1459530.1740380801801459No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1423700.16976561958471134No Hit
TCGACTGCATAATTTGTGGTAGTGGGG1408170.16791378277067007No Hit
AGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTAT1401000.16705881368137993Illumina Paired End PCR Primer 2 (100% over 38bp)
GGGAGAGCGACGCTCAGACAGG1364360.16268976661979123No Hit
CCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTC1363420.16257767862203212No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGC1345270.16041342632487507No Hit
GGGAGAGCGACGCTCAGACAG1321010.15752060204228385No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1313350.15660720410309803No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1224570.14602085044240357No Hit
CGTCCCCGCTCGGCGGGGTCCCCGC1216150.145016828164604No Hit
GGGAGAAAGCCCCC1202440.14338201279303245No Hit
ACCTCTAGCATTACAAGTATTAGAGGC1167220.1391822901535905No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTA1154160.13762498244004387No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCC1153490.1375450899310028No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT1128740.1345938367985159No Hit
CCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCG1121730.13375794651735495No Hit
GGGAGAGCGACGCTC1102500.13146491226532572No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCG1090270.13000657586894937No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCG1082960.12913491282254616No Hit
CGAAGCCGAGCGCACGGGGTCGGCGGCGA1078890.12864959564075945No Hit
GGGAGAGATC1077110.128437343900322No Hit
CGGTTGGCCCCGGATAGCCGGGTCCC1053750.1256518379134576No Hit
ACCCCGAGTGTTACAGCCCTCCGG1019000.12150815927289517No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC1004150.11973740739340304No Hit
CCCTGGTGGTCTAGTGGTTAGGATT983790.11730962905896127No Hit
AGGGAGAGATCC976510.11644154328908231No Hit
CCTCCGCGCGCCGGGTTTCGCCCCCGCGGCG964010.11495101140398792No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA892400.10641205234065913No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAG890620.10619980060022169No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA885440.10558212418703856No Hit
GGGAGAGTTCCCCC884710.10549507712494906No Hit
GGGAGAGCGACGCTCA880230.10496087049733122No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAG878350.10473669450181303No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGG870370.10378513894636876No Hit
GGGAGAGCCTC862710.10287174100718294No Hit
GGGAGAAAGC841540.10034737620658704No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)