FastQCFastQC Report
Tue 19 Sept 2023
SRR17238157_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR17238157_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65086832
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length5-38
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGAGATCC14131952.1712456369054802No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGG13389862.0572302551151975No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC11443851.7582435107611323No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGC8710501.3382891335070664No Hit
GGGAGAAAGCCC8278961.2719869358520937No Hit
GGGAGAAAGCC8254581.2682411705028138No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACC7684561.180662779838478No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCG7002351.0758474156492976No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGC6474470.9947434528692378No Hit
GGGAGAAAGCCCC4116620.6324812367576901No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCG3619730.5561386057321088No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAC3112220.4781643082582357No Hit
GGGAGAGCGACGCTCAGAC2947960.4529272526276897No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC2887090.4435751305271702No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCG2594220.3985783176541762No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCG2383310.3661739136420098No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCG2205730.3388903611102166No Hit
GGGAGAGCGACCCC2191720.33673785198210293No Hit
GGGAGAGCGACGCTCAGACA2011070.3089826218612084No Hit
GGGAGAGCGACCCCC1961590.3013804697085272No Hit
GGGAGAGCCTC1944360.29873323685503694No Hit
GGGAGAGCGACGCTCAGACAGGCGTAGCCCCGGGAGGA1917490.29460490564358705No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGA1831020.28131957628541515No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGC1817640.27926386092965777No Hit
CCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTC1817610.27925925170240273No Hit
TCGCTTCTGGCGCCAAGCGTCCG1797610.2761864335323618No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGA1791800.2752937798539649No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAG1754770.2696044570121342No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG1746530.26833845592607736No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGC1731640.2660507427984819No Hit
GGGAGAGCGACGCTCAGA1724020.26487999907569626No Hit
GGGGAGAGATCC1607610.24699466091697317No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC1583940.24335798061272976No Hit
CTCGCTTCTGGCGCCAAGCGTCCG1575330.24203513239052718No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCG1542460.23698495572806494No Hit
GGGAGAAAGCCCCC1522940.23398588519410501No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC1498350.2302078552540397No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC1486600.22840257457914068No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTT1484590.22809375635305154No Hit
GGGAGAGCGACGCTC1455020.22355059468864608No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCC1405610.21595919739956002No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCG1353380.2079345327484982No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1265290.19440030511855302No Hit
GGGAGAAAGC1241460.19073904226894928No Hit
GGGAGAGATC1206680.18539541147124813No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTA1170800.17988277567419472No Hit
GCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCC1162370.1785875828155225Illumina Paired End PCR Primer 2 (100% over 38bp)
TCGGTTGGCCCCGGATAGCCGGGTCCCCG1155620.1775505066831337No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCG1147820.17635210759681774No Hit
GGGAGAGCGACGCTCAGACAGG1147620.17632137941511733No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCC1141730.1754164344640403No Hit
GGGGAGAGCCTC1140260.17519058232854226No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA1130840.17374328497045302No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT1112570.17093626557212063No Hit
GGGAGAGCGACGCTCAGACAG1091640.16772056135717284No Hit
AGGGAGAGATCC1084600.1666389293613184No Hit
GGGAGAGCGACGCTCA1070060.1644049905516987No Hit
CCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTC1034520.158944592663536No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC1016960.15624665831024007No Hit
GGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAC988630.15189401137237712No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG982590.1509660202850248No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGA972860.1494710942452999No Hit
CGTCCCCGCTCGGCGGGGTCCCCGC965990.14841558120389084No Hit
CCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCG962090.14781638166073285No Hit
TCGACTGCATAATTTGTGGTAGTGGGG907090.13936613169312034No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGG886550.13621034743248833No Hit
AAGCTATTAATGGTTCGTTTGT882120.13552971820782428No Hit
GGAGAGATCC873600.13422069766738687No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCAG858890.13196063990332177No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC796200.12232889134932855No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC793880.1219724444416038No Hit
GGGAGAGATCCTTCCGCAGGTTCACCTACGGAAACCTT792130.12170357285172521No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC767480.11791632445714981No Hit
GGGAGA765650.11763516159459106No Hit
GGGAGAGTTCCCCC762430.11714043786921448No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG756360.11620783755460706No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCG753120.11571004101106042No Hit
AGGTCGGTTTCTATCTATTTACGATTTCTC738000.11338699047450951No Hit
GGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGC736790.11320108497522204No Hit
ACCTCTAGCATTACAAGTATTAGAGGC681800.10475237141669455No Hit
ACCCCGAGTGTTACAGCCCTCCGG667220.10251228697073472No Hit
AGCTATTAATGGTTCGTTTGT660860.10153513079266172No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAA653750.10044274393321218No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)